巻号一覧
64 巻 (1997)
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64 巻, 4 号
選択された号の論文の14件中1～14を表示しています
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仲間 一雅1997 年 64 巻 4 号 p. 287-291
発行日: 1997/08/15
公開日: 2009/07/10
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Takero Takamura, Atsuhiro Sakamoto, Ryo Ogawa1997 年 64 巻 4 号 p. 292-297
発行日: 1997/08/15
公開日: 2009/07/10
ジャーナル フリーIt is well known that both local and volatile anesthetics depress myocardial functions. This study was performed to evaluate the combined effects of commonly utilized local anesthetics (bupivacaine, mepivacaine, and lidocaine) and a volatile anesthetic (isoflurane) on heart rate and myocardial contractility in an isolated rat working heart preparation using each local anesthetic at 0 to 3×10-4 (mol/L) with or without 1 MAC (minimum alveolar concentration) of isoflurane. The three local anesthetics depressed the heart rate and myocardial contractility dose-dependently. Bupivacaine depressed the heart rate more than the other two local anesthetics. One MAC isoflurane showed significant negative inotropic effects, and accelerated the effects of the local anesthetics. By using isobolographic analysis, we concluded that the combined effect of isoflurane and bupivacaine on myocardial negative inotropism was synergistic, while the effects of isoflurane and mepivacaine or lidocaine were additive. (J Nippon Med Sch 1997; 64:292-297)抄録全体を表示PDF形式でダウンロード (454K) -
ウサギによる組織学的,分子生物学的検討飯澤 典茂1997 年 64 巻 4 号 p. 298-306
発行日: 1997/08/15
公開日: 2009/07/10
ジャーナル フリーAn experimental study was performed to investigate the histological and molecular biological properties of reconstructed anterior cruciate ligaments (ACL) using the patellar tendon augmented by absorbable artificial material in rabbits. Thirty rabbits underwent ACL reconstruction with the patellar tendon (non-augmented group), and 30 with the patellar tendon augmented by a polyglactin 910 mesh (augmented group). They were respectively sacrificed at 1, 2, 3, 4, 6, 8, and 12 weeks after surgery for histological and molecular biological examination. Histologically, the fibrocytes were disappeared in deep portion of transplanted graft at 2 weeks after surgery in both groups. The graft was replaced with granulated tissues at 6 weeks in the augmented group, but it took 8 weeks in the non-augmented group. At 12 weeks, the graft showed hypocellularity with regularly oriented fibers in the augmented group, while it still showed hypercellularity with disoriented fibers in the non-augmented group. Molecular biologically, after the reconstruction at one week, the relative radioactivity in the graft was 1.05 in the non-augmented group, and 2.10 in the augmented group. Additionaly the level of the a 2 (I) collagen mRNA was also significantly higher in the augmented group than that in the non-augmented group. These results suggested that a polyglactin 910 mesh had an effect on the process of reorganization and remodeling in the transplanted patellar tendon during the post-operative period in rabbit. (J Nippon Med Sch 1997; 64: 298-306)抄録全体を表示PDF形式でダウンロード (7813K) -
Seiji Gomi, Setsuo Hasegawa, Kazuo Dan, Ichiji Wakabayashi1997 年 64 巻 4 号 p. 307-313
発行日: 1997/08/15
公開日: 2009/07/10
ジャーナル フリーHuman umbilical cord blood (UCB) is currently considered as a third source of hematopoietic stem cells for transplantation, following bone marrow and growth-factor-mobilized peripheral blood (MPB). To evaluate the potential benefits of UCB, we performed a comparative study of the properties of the stem cells in UCB and MPB samples. CD 34+ cell determination and CFU-GM colony assay showed a lower frequency of committed progenitors in UCB than in MPB. In contrast, a higher level of the CD 34+ CD 38- subset in UCB suggested that more primitive, multipotent progenitors are enriched in UCB than in MPB. Phenotypic analysis of UCB lymphocytes revealed a reduced level of T cell subsets, especially cytotoxic CD 8+ lymphocytes, which might minimize graft versus host disease in clinical practice. In conclusion, UCB is an attractive alternative source for stem cell trans-plantation, but ex vivo expansion of stem/progenitor cells could be effective for attaining rapid and safer hemopoietic reconstitution. (J Nippon Med Sch 1997 ; 64: 307-313)抄録全体を表示PDF形式でダウンロード (445K) -
Yasuichiro Fujita, Masayo Saito1997 年 64 巻 4 号 p. 314-322
発行日: 1997/08/15
公開日: 2009/07/10
ジャーナル フリーIn cats, anesthetized with pentobarbital sodium and immobilized with d-tubocurarine, it was discovered by changing the strength of L 7 or S 1 ventral root (VR) stimulation that the stimulation could produce in the same motoneuron the IS-spike, i.e., the spike of the initial segment, either via the M-spike or without the M-spike, which is believed to be the spike of the node of Ranvier as recorded in the soma. It was shown by simultaneously recording the compound action potential of the L 7 or S 1 ventral root that the threshold of VR stimulation to produce the IS-spike via the M-spike was higher than that to produce it without the M-spike in all the 18 motoneurons in which the threshold was studied. With weaker stimulation the M-spike was not produced and the IS-spike arose from a potential smaller than the M-spike (the M-potential) or directly from the baseline. The M-potential could also trigger the M-spike. These results disclose the possibility that the M-spike originates not in the axon, but somewhere else such as the dendrite. (J Nippon Med Sch 1997; 64: 314-322)抄録全体を表示PDF形式でダウンロード (608K) -
Possible dendritic spikesYasuichiro Fujita, Masayo Saito, Kaoru Kobayashi1997 年 64 巻 4 号 p. 323-328
発行日: 1997/08/15
公開日: 2009/07/10
ジャーナル フリーExtrasomatic (ES-) spikes were recorded in motoneurons in cats anesthetized with pentobarbital sodium and immobilized with d-tubocurarine. ES-spikes, 40-79 mV in amplitude, were distinguished from intrasomatic spikes by their differences in responses to a train of 8 pulses at 500 Hz given to the L 7 or S 1 ventral root. In 23 motoneurons 3-6 different spikes could be recorded from the same site in the same cell in response to the ventral root stimulation. It was not possible to explain these multiple spikes as axon spikes or IS-(initial segment) spikes because too many spikes were observed at the same site. Accordingly, it was suggested that they were dendritic in origin. (J Nippon Med Sch 1997 ; 64: 323-328)抄録全体を表示PDF形式でダウンロード (430K) -
A morphological studyYu-zhi Jia, Shigeru Sato1997 年 64 巻 4 号 p. 329-336
発行日: 1997/08/15
公開日: 2009/07/10
ジャーナル フリーHistological changes were examined in the left ventricular free wall of the heart in 40 rats after ligation of the distal anterior descending coronary artery for 15, 30 minutes and 1, 2, 3, 4, 5, 6 hours. Auto-injection of tracers for light microscopy was used to examine the development of collateral circulation. Morphological changes of the ischemic myocardium were observed by PAS staining and transmission electron microscopy. Fifteen minutes after coronary occlusion, the dye was observed in the vein of the ischemic zone; however, 30 minutes after coronary occlusion, the dye appeared in the capillaries of the outer-third layer. These findings suggested that the collateral circulation becomes functional between 15 and 30 minutes after coronary occlusion. Collateral circulation increased gradually in the capillaries of the ischemic zone. Thirty minutes after coronary occlusion the dye was seen only in the outer-third layer of the left ventricular ischemic zone. One hour after occlusion, the dye appeared in the superficial space and the middle-third layer. Three hours after occlusion, the dye was seen in all layers of the ischemic zone. The dye appeared in the ischemic zone, where myocardium damage was not homogeneous. The positive reaction obtained by PAS staining corresponded with the capillaries, veins and superficial spaces in which the dye was evident. These results suggest that collateral circulation, venural back flow and superficial space flow are able to prevent myocardial infarction in early ischemia. (J Nippon Med Sch 1977 ; 64: 329-336)抄録全体を表示PDF形式でダウンロード (10548K) -
Yasuichiro Fujita, Masayo Saito1997 年 64 巻 4 号 p. 337-343
発行日: 1997/08/15
公開日: 2009/07/10
ジャーナル フリーVentral root stimulation is known to produce two spikes, large and small, in the α-motoneuron. Since it is the small spike that triggers the outgoing impulse from the cell it is regarded as the key potential in information processing of the cell and referred to either as the IS-spike or as the A-spike depending upon whether one considers it as a purely single spike or as a composite spike. The small spike was elicited by stimulating the L 7 or S 1 ventral root in cats anesthetized with pentobarbital sodium and immobilized with d-tubocurarine. During the later phase of the excitatory prostsynaptic potential and thereto following inhibitory postsynaptic potential (IPSP) produced by stimulating the corresponding dorsal root the amplitude of the small spike was reduced by about 20%. This could be either explained as due to reduction in impedance of the membrane generating the small spike or interpreted as suggesting that the small spike is a composite spike and some of its components were inhibited by the IPSP. This interpretation was favored because the reduction occurred not when the safety factor for antidromic impulse transmission was high, but when it was low. (J Nippon Med Sch 1997; 64: 337-343)抄録全体を表示PDF形式でダウンロード (470K) -
スクリーニングの有用性についての研究犬塚 祥, 林田 真喜子, 仁平 信1997 年 64 巻 4 号 p. 344-352
発行日: 1997/08/15
公開日: 2009/07/10
ジャーナル フリーThe results of toxicology screening of samples from 725 patients admitted to the Critical Care Medical Center (CCMC) of Nippon Medical School during a 10-month period from 1992-1993 (Group A) and a 4-month period from 1995-1996 (Group B) were discussed. We investigated the drug use of emergency patients using immunoassay, EMIT and Triage. The results were confirmed by Gas Chromatography/Mass Spectrometry (GC/MS). Blood samples were analyzed for ethanol (EtOH) by head space gas chromatography.
Overall, 18% of the 725 cases tested positive for drugs, 13% for EtOH. Recently the positive rates of drugs and EtOH have been increasing. The positive rates for drugs in Group A and Group B were 15% and 23%, and EtOH were 11% and 17%, respectively. False positive cases caused by the cross-reactivity to analog were found in both EMIT and Triage. But the reliability of both methods was sufficient for clinical use. Rapid in easy toxicology screening can provide useful clinical information for patients admitted to a CCMC, especially for patients who have been injured, have sustained unknown-etiology consciousness disturbances, have CPAOA (Cardio Pulmonary Arrest on Arrival) or have committed drug abuse. We conclude that toxicology screening using immunoassay methods is suitable for use in an emergency center. (J Nippon Med Sch 1997; 64: 344-352)抄録全体を表示PDF形式でダウンロード (610K) -
平川 恒久1997 年 64 巻 4 号 p. 353-355
発行日: 1997/08/15
公開日: 2009/07/10
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浅野 健, 前田 美穂, 山本 正生1997 年 64 巻 4 号 p. 356-358
発行日: 1997/08/15
公開日: 2009/07/10
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Yoshikazu Genbun, Yasumasa Shirai, Masabumi Miyamoto, Kazuhiro Kaneda, ...1997 年 64 巻 4 号 p. 359-361
発行日: 1997/08/15
公開日: 2009/07/10
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1997 年 64 巻 4 号 p. 362-374
発行日: 1997/08/15
公開日: 2009/07/10
ジャーナル フリーPDF形式でダウンロード (1082K) -
石渡 俊行1997 年 64 巻 4 号 p. 375-376
発行日: 1997/08/15
公開日: 2009/07/10
ジャーナル フリーPDF形式でダウンロード (1590K)
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