巻号一覧
63 巻 (1996)
- 6 号 p. 441-
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- 2 号 p. 79-
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63 巻, 2 号
選択された号の論文の10件中1～10を表示しています
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岡崎 太郎, 大網 弘1996 年 63 巻 2 号 p. 79-93
発行日: 1996/04/15
公開日: 2010/03/01
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太田 成男, 猪原 直弘1996 年 63 巻 2 号 p. 95-105
発行日: 1996/04/15
公開日: 2010/03/01
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菅 隼人, 恩田 昌彦, 田中 宣威, 古川 清憲1996 年 63 巻 2 号 p. 106-116
発行日: 1996/04/15
公開日: 2010/03/01
ジャーナル フリーWe studied the anti-tumor effect of green tea polyphenol fraction (SunphenonTM, SF: provided by Taiyo Kagaku Inc., Mie, Japan) on DMH-induced colorectal carcinogenesis in male Wistar rats. DMH was subcutaneously administered weekly at 20 mg/kg for 14 weeks. The rats in group I (20 rats) were given tap water for the whole of the study period. The rats in group II (15 rats) were given tap water from weeks 0-14, and 0.1% SF from weeks 15-35. The rats in group III (21 rats) were given 0.1% SF during the whole period. The rats were sacrificed at week 35. The cecal contents were aseptically removed and examined microbiologically to obtain the counts of four bacteria species (including Clostridium perfringens) per 1 g of cecal contents.
The incidence of tumors production was significantly decreased (Group I: 100% vs Group II: 57.1%, Group III: 62.5%, p<0.05), and the frequency of occurrence of C. perfringens (which is thought to yield harmful products which may be carcinogenic) was decreased in the SF-treated groups. These results suggest that SF prevents DMH-induced carcinogenesis in rats, and that its effect may be somehow related to its ability to preserve the composition of the colonic microflora.抄録全体を表示PDF形式でダウンロード (1926K) -
福地 孝明, 片山 泰朗, 赫 彰郎1996 年 63 巻 2 号 p. 117-126
発行日: 1996/04/15
公開日: 2010/03/01
ジャーナル フリーPyruvate dehydrogenase (PDH) is one of the mitochondrial enzymes which regulate the glucose metabolism. The purpose of this study is to determine the effect of the duration of cerebral ischemia on PDH activity and the metabolites.
Cerebral ischemia was produced by bilateral common carotid artery occlusion in Mongolian Gerbils. 20-minute (1) and 60-minute ischemic groups (2) were made. PDH activity and energy metabolites (ATP, PCr, lactate) were measured in the caudate nucleus and cortex at each time period.
1) 20min ischemic group: PDH activity significantly increased after 20-min ischemia in both the caudate nucleus and cortex, and decreased to levels less than that of the control after 20 min reperfusion. At 60 and 120min reperfusion, PHD activity returned to the control levels. ATP and PCr concentrations were significantly depleted after the ischemic insult, returning to 60-80% of the control level after reperfusion. Lactate concentrations increased significantly after ischemia, and were reduced by reperfusion.
2) 60min ischemic group: PDH activity significantly increased after 60min ischemia, and decreased but remained higher than the control level after 20min reperfusion. At 60 and 120min reperfusion, PDH activity gradually decreased towards control levels. ATP and PCr concentrations were depleted after ischemia, and were gradually restored after 20min reperfusion, recovering to 50% after 60min reperfusion. Lactate concentrations increased after the ischemic insult, and became more elevated after reperfusion.
These findings indicate that there is a significant difference in the PDH activity and metabolism depending on the duration of ischemia. The data suggest that impaired metabo- lism and persistent elevation of PDH activity may be caused by demage to the mitochondria allowing the influx of Ca2+ during prolonged ischemia.抄録全体を表示PDF形式でダウンロード (1489K) -
亀山 元帥1996 年 63 巻 2 号 p. 127-139
発行日: 1996/04/15
公開日: 2010/03/01
ジャーナル フリーLocus control region (LCR) is known to occur 5′-upstream of the globin gene clusters in humans and a number of other animals. It comprises four DNase I hypersensitive sites, HS 1-4, and has been considered to play a key role in regulating the globin gene expression in tissue- and developmental stage-specific manners. The occurrence of LCR in the rat genome, however, has not been documented so far. In the present study, the author intended to identify and analyze the rat β-LCR HS 1 and HS 2, in order to further facilitate studies on the regulatory mechanism involved in globin gene expression. The results obtained in this study are summerized as follows:
1. A DNA region of about 700 bp on the rat genome was amplified by polymerase chain reaction (PCR) using synthetic primers derived from portions of the mouse β-LCR HS 2. The nucleotide sequence of the PCR product (R 700) shows 67% and 83% homologies with those of the human and mouse HS 2, respectively, indicating that R 700 represents β-LCR HS 2 of rats.
2. In order to locate β-LCR HS 2 on the rat genome, a 7 kb DNA fragment (R 7, 000) harboring a region between β-LCR HS 2 and the ε 1-globin gene was obtained by PCR. Restriction endonuclease mapping of R 7, 000 revealed that the rat β-LCR HS 2 is located 6.0kb 5′-upstream relative to the cap site of the ε 1-globin gene.
3. The rat β-LCR HS 1 was then located 4.2 kb 5′-upstream of the ε 1-globin gene by Southern blot hybridization of R 7, 000 using a human HS 1 probe. Nucleotide sequencing revealed that the rat HS 1 has 83% homology to the mouse HS 1.
4. Comparisons of the structures of the rat β-LCR HS 1 and HS 2 with those of other animal species indicate that several motifs and consensus sequences for binding of transcription factors, such as NF-E 2/AP-1 and GATA-1, are well conserved during evolutional periods, indicating an indispensable role of LCR in globin gene expression抄録全体を表示PDF形式でダウンロード (3145K) -
杉本 忠彦1996 年 63 巻 2 号 p. 140-153
発行日: 1996/04/15
公開日: 2010/03/01
ジャーナル フリーIn order to induce either atrial fibrillation (af) or atrial flutter (AF) in 22 dogs under pentobarbital anesthesia, a right atriotomy parallel to the sulcus terminalis was performed, but closed with running sutures. Effective refractory periods (ERP) were measured in the sites of the appendages and the bodies in both atria. Further, we developed a computerised mapping system which enabled us to reveal sequential activation of various parts of the heart. As a result, we could assess the circuit of excitation which gave rise to AF. AF occurred in 7 of the 22 dogs, and af in the remaining 15 dogs. It was revealed that during AF there was a counterclockwise (5 dogs) or clockwise (2 dogs) reentrant pathway involving the right atrial free wall and superior vena caval orifice with consequent left atrial activation. Although standard limb lead electrocardiogram showed af, all dogs with sustained af had a regular activation sequence in the left atrium [at a cycle length (period) of 140.1±16.9 msec, arising either in the left atrial appendage (8 dogs) or ajacent to the left pulmonary vein (7 dogs)]. However, 12 of the 15 dogs had a chaotic activation sequence whereas the other 3 dogs showed a regular activation sequence in the right atrium. Statistically, during AF there was no significant difference between RA ERP (153.2±12.6 msec), LA ERP (146.4±14.4 msec) and LA cycle length (period) (140.1±16.9 msec). During af, there was no significant difference between LA ERP (131.7±15.2 msec) and LA cycle length (period) (125.7±9.1 msec), but RA ERP (165.4±15.5 msec) was significantly longer than the LA cycle length (period) (p<0.0001).
Although in the present experiment regular left atrial activation and complex right atrial activation were observed predominantly, the activation sequence of af was different in each dog. These results clearly indicate that to employ a mapping system like ours is necesary to determine relevant surgical procedures to stop af and AF.抄録全体を表示PDF形式でダウンロード (2297K) -
Takao Endo, Haruo Kaneko, Kaname Kiuchi, Shinsuke Fujita, Takeshi Yama ...1996 年 63 巻 2 号 p. 154-160
発行日: 1996/04/15
公開日: 2010/03/01
ジャーナル フリーTo examine whether endothelial nitric oxide formation contributes to the vascular tone of resistance coronary vessels in vivo, we administered NG-nitro-L-arginine methyl ester (L-NAME) (10 and 100μg/kg/min), a nitric oxide synthase inhibitor, as well as D-enantiomer into the left circumflex artery in normal dogs. Intracoronary L-NAME, which was associated with dose-related reductions in acetylcholine-induced coronary vasodilation, significantly reduced the baseline left circumflex blood flow by 6% and increased coronary vascular resistance of the left circumflex artery by 6%. D-enantiomer was ineffective in altering baseline coronary blood flow and vascular resistance of the left circumflex artery. These results indicate that continuous nitric oxide formation in the vasculature is important in the regulation of the coronary vascular tone of resistance vessels in vivo, and serves to maintain the vessels in a dilated state.抄録全体を表示PDF形式でダウンロード (1021K) -
高崎 秀明, 恩田 昌彦, 古川 清憲1996 年 63 巻 2 号 p. 161-164
発行日: 1996/04/15
公開日: 2010/03/01
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Shin-ichi Koumi, Ryoichi Sato1996 年 63 巻 2 号 p. 165-168
発行日: 1996/04/15
公開日: 2010/03/01
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Lertlakana Bhoopat, 石丸 新, 田中 茂夫, 高橋 秀実, 渡辺 誠, 朝倉 啓文1996 年 63 巻 2 号 p. 169-172
発行日: 1996/04/15
公開日: 2010/03/01
ジャーナル フリーPDF形式でダウンロード (864K)
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