The gels using cell embedding is require of the profiles of bio-compatibility and selective transport properties. In usually, it is difficult that we build one materials of having the both properties. In this works, we tried preparation of the laminated gel of agarose gel and acrylamide gel. Agarose gel have a promotion of bio-compatibility, because this gel is not decomposed by enzyme in vivo. Polyacrylamide gel is easy control mass transport properties. The mass transport properties as gel evaluated from the diffusion coefficient of probe molecular. Probe moleculars are used glucose, vitamin B₁₂, and bovine serum albumin. The diffusion coefficients of probe molecules in gel were determined by the time-lag technique. The laminated gel of agarose gel and polyacrylamide gel is blocked the permeation of macromolecular. Further, the permeation of low molecular throw the laminated gel was similar as that of agarose gel. These results is suggestion the impossible to create the cell embedding gel as the combine to both gel at biocompatibility and selective permeability. These informations obtained by the transport experiments are necessary to design the bio-interface materials

View full abstract

As concepts of myocardial protection evolve in search for the ideal method, systems to deliver the proper perfusate temperature on a very short notice are needed. In spite of the small capacity of the newly designed disposable heat exchanger (41ml) consisting of a small multicoil unit immersed in a controllable manner in a bucket of iced water, it is capable of providing almost instantaneous deliver of blood practically at any desired clinically applicable temperature. If intermittent blood cardioplegia is accepted, just adding a perfusion line in the blood re-circulation line allows a single system to be shared for alternate cardioplegic blood and selective brain perfusion at two desired temperatures, thus obviating the need for a separate brain pump.

View full abstract

Systems to deliver the proper perfusate composition at the proper temperature on demand are needed to keep up with the evolving brain and myocardial protection concepts. A system with a rather small total priming volume (120ml) incorporating a) a new one pass effective heat exchanger in the blood perfusion line upstream to the cardioplegic additive mixing point, and b) debubling mechanisms in the chamber (s) holding the variable composition additive solution (s), as well as in the blood recirculation lines allow administration of either unmodified blood or the oxygenated basic crystalloid solution with or without mixing the additive (s) using the same conventional pump head. By accepting intermittent (cold or warm) blood cardioplegia, the same circuit and pump head could be shared for intermittent cold blood selective brain perfusion. The same principle is applicable for alternate perfusion of a hemoconcentrator. Implementing selective brain or hemoconcentrator perfusion is very simple and possible even after cardiopulmonary bypass (CPB) has been initiated which results in marked simplification of the CPB circuit and equipment, and consequent reduction of priming volume, and savings of energy and time.

View full abstract