By using the polarographic technique with a rotating electrode, respiratory function was tested in heart mitochondria which were obtained from guinea pigs pretreated with sympathomimetics and adrenergic blocking agents. The degree and duration of mitochondrial impairment were dose-dependent. Alpha and beta adrenergic blocking agents both induced the same type of obstruction of mitochondrial respiration as was seen with sympathomimetics. It has been suggested that a hypoxic state of the myocardium induced by sympathomimetics is partially related to the onset of respiratory obstrution in myocardial mitochondria. No relationship was observed between the catecholamine-induced impairment of mitochondria and adrenergic receptors in the myocardium.
Detoxication of bis (2-hydroxy-3, 5-dichlorophenyl) sulfide, bithionol, was investigated in man. A novel metabolite was isolated as a crystalline derivative from bithionol medicated urine and identified as bithionol-monoglucuronide (as a methyl acetyl derivative). Oxidation of the sulfur atom of this compound was not recognized It is concluded that bithionol is detoxicated partially by forming a monoglucuronide conjugate.
Using whole body- and micro-radioautography it was found that radio-activity was specifically retained for more than a month following an i.v. injection of 14C-chloroquine in the retinal pigment epithelium of pigmented rats and guinea-pigs, but not in that of albino animals. By a long term oral administration of chloroquine the intensive accumulation of melanin granules in the pigment epithelium and also in the choroid of the pigmented guinea-pig was observed. Using synthetic melanin, chloroquine was found to be adsorbed by melanin in two modes; one operates in low chloroquine concentrations and is extremely strong and independent to pH, and the other operates in higher chloroquine concentrations and is pH-dependent and chloroquine can be released by washing.
Sanpoly-305 was mixed in feed at 3 different concentrations-100ppm, 1000ppm, and 2500ppm-and fed to rats for 79 weeks. Animals did not show any symptoms up to 52-week except for occasional decrement of general state by murine pneumonia. From 53-week on, they showed so called senile changes as discharge of viscous eye mucus, hypokinesia and etc., but there were no specific symptoms related to the agent administration. No specific effect related to the agent administration was not seen in incidence and types of the tumors occurred in the present study.