^3H-labeled lentinan was prepared by methylation with
3H-dimethyl-sulfate. Blood level, distribution and excretion were studied in rats. After intravenous injection of [methyl-
3H]-methyl lentinan, radioactivity rapidly disappeared from blood, lung and kindney, distributed mainly in liver, spleen and mesenteric lymph nodes. There was urinary excretion, but expiratiry excretion was a small proportion. In the case of multi-injection of [methyl-
3H]-methyl lentinan distribution of radioactivity was similar to a single injection, but retention of
3H was less than a single injection. Bile excretion and transport to fetuses and milk were in mere trace amounts. No substantial difference was found between two types of the
3H-labeled lentinan in either the distribution or excretion.
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