In 2016, 2 methods categorized in JOCS Standard Method, 2.4.14-₂₀₁₆ and 2.4.5-₂₀₁₆, and 1 method categorized in JOCS Methods for Reference, S2.2.2.2-₂₀₁₆, have been added to Standard Methods for the Analysis of Fats, Oils and Related Materials (2013 Edition). Also in 2017, 1 method categorized in JOCS Recommended Method will be added. These newly registered test methods will be published in 2018 and will be sold to the test method user as the Revised and Enlargement Edition for the 2013 Edition. The following is the outline of these test methods.

Analytical methods for 2-/3-MCPD and Glycidyl fatty acid esters in edible oils and foods have been developed in various countries. Those include direct methods based on measurement of every ester using LC-MS, and indirect methods based on hydrolysis of esters and subsequent measurement of 2-/3-MCPD and glycidol in free-form using GC-MS. Indirect methods have an advantage over direct methods because they can be used for simultaneous determinations of 2-/3-MCPD and glycidol esters as their free-form equivalents. The enzymatic method that uses a lipase from Candida rugosa for the hydrolysis is the fastest among the indirect methods available today. The ester hydrolysis completes in 0.5 hour, and the preparations of analytical samples and calibration curves are simple and easy. Japan Oil Chemists’ Society (JOCS) conducted a collaborative study with 13 participant laboratories, and obtained results with good reproducibility. The method is registered in The JOCS Standard Methods for the Analysis of Fats, Oils and Related Materials as 2.4.14-₂₀₁₆.

The previous enzymatic methods to analyze fatty acid composition at sn-2 (β) position in triacylglycerols (TAG) using hydrolysis of pancreas lipase, ISO 6800 : 1997, or of microbial lipase, JOCS Recommended Method R2-₂₀₁₃, cannot be applied to TAG containing short chain and polyunsaturated fatty acids due to the properties of the lipases. A new method using α-position-selective transesterification by Candida antarctica lipase B (CALB) is applicable to TAG with short chain and polyunsaturated fatty acids, and approved as JOCS Standard Method 2.4.5-₂₀₁₆. This paper introduces the principle and the procedure of the standard method. In addition, the propyl esterification is effective to analyze the contents of short chain fatty acids with good reproducibility. The method was approved as JOCS Methods for Reference S 2.2.2.2-₂₀₁₆.