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Volume 46 (1994)
- Issue 6 Pages 689-
- Issue 5 Pages 559-
- Issue 4 Pages 379-
- Issue 3 Pages 283-
- Issue 2 Pages 165-
- Issue 1 Pages 1-
Volume 46, Issue 5
Displaying 1-6 of 6 articles from this issue
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Tamotsu IBARAKI, Shigeru SAITO, Motohiko ICHIJO1994 Volume 46 Issue 5 Pages 559-568
Published: September 01, 1994
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSBy the polymerase chain reaction (PCR), we have developed a system for type-specific (human papilloma virus (HPV) -16, 18 and 33) as well as universal detection of HPV. We applied this system to 120 uterine cervical tissue specimens, and detected HPV DNA in 84% (41/49) of the carcinomas, 61% (20/33) of the CIN and 3% (1/38) of the normal cervical tissues. To clarify the association between p53 mutation and HPV infection, the presence of p53 mutation was examined by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). By this assay, we detected no mutations in the p53 gene in 14 cervical carcinomas (9 HPV-positive, 5 HPV-negative). This suggests that inactivation of p53 by point mutation is infrequent in cervical carcinomas irrespective of the presence or absence of HPV infection.
[Adv Obstet Gynecol 46 (5); 559-568, 1994 (H6.9)]View full abstractDownload PDF (6514K) -
Shinji URAKAWA, Katsuhiko YASUDA, Yorihiko HORIKOSHI, Isamu SAWARAGI1994 Volume 46 Issue 5 Pages 569-578
Published: September 01, 1994
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSProstaglandins (PGs) play an important role in pregnancy, labor and delivery, especially in the onset and progress of labor. We measured and identified PGs and their metabolites in the urine during pregnancy, labor, and the puerperium. PGs and their metabolites were measured using derivatization with anthryldiazomethane (ADAM) and high performance liquid chromatography (HPLC).
Urine samples were collected from pregnant women at various stage of gestation, postpartum women (1, 3 and 5 days after delivery), three nonpregnant women and two men.
PGs and their metabolites were extracted from urine with octadecylsilyl silica by the method of Powell and re-extracted to remove substances that interfered with derivatization using ADAM. The ADAM derivatives of PGs and their metabolites were applied to reverse phase HPLC under condition A (solvent system, methanol : water 75 : 25 (v/v); flow rate, 1ml/min) and condition B (solvent system, acetonitrile : water : phosphoric acid 60 : 39.9 : 0.1 (v/v/v); flow rate, 1ml/min) and detected using a fluorescence spectrophotometer (EX 365 nm, EM 412 nm).
Specific chromatographic peaks were observed in urine samples obtained from the pregnant women after more than five weeks of gestation, but were not observed in the samples obtained from nonpregnant women and men. In addition, the specific urinary peaks in the pregnant women disappeared within five days after delivery. The retention times of the specific chromatographic peaks were 54 min, 58 min, 62 min, 67 min, 71 min under condition A, while the times were 37 min, 39 min, 43 min, 57 min, and 61 min under condition B.
PGE2, PGF2α, 15-keto-PGE2, 15-keto-PGF2α, 13, 14-dihydro-15-keto-PGE2, 13, 14-dihydro-15-keto-PGF2α, metabolites of estrogen and progesterone, malonic acid, oxalic acid, and hippuric acid were also studied using delivertization with ADAM and HPLC. However, none of the peaks of these substances were consistent with the specific peaks in the urine of pregnant women. These findings suggest that the urinary peaks obtained in our pregnant women were pregnancy-specific. However, identification of the substances that produce these pregnancy-specific peaks has not yet been achieved and further studies are needed for this purpose. [Adv Obstet Gynecol 46 (5); 569-578, 1994 (H6. 9)]View full abstractDownload PDF (1299K) -
Sri Kadarsih SOEJONO, Hajime MORIKAWA, Matsuto MOCHIZUKI1994 Volume 46 Issue 5 Pages 579-587
Published: September 01, 1994
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSThe differences in thyroid status in pregnant women during the third trimester, and the influences on fetal growth were studied in both an endemic goiter area (EGA group in the Ponjong area) and a nonendemic goiter area (NEGA group in the Tepus area) in Indonesia. From each area, 30 pregnant women in the third trimester and 30 nonpregnant women as controls were recruited. Serum concentrations of free T3, free T4, TSH, free fatty acid (FFA), total protein, and glucose, and newborn birth weight were measured. It was found that in nonpregnant women, serum free T4 levels were significantly lower in the EGA group than in the NEGA group (p <0.001), and serum free T4 levels of pregnant women were significantly lower than those of nonpregnant women, either in the NEGA group (P<0.001) or in the EGA group (p<0.05). However, maternal serum free T3 and free T4 levels during the third trimester in the EGA group were significantly higher than those in the NEGA group (p<0.001, p<0.001, respectively). Maternal serum levels of FFA and total protein were not significantly different between the two groups, but maternal blood glucose levels in the EGA group were significantly higher than those in the NEGA group, and newborn birth weights in the EGA group were significantly higher compared with those in the NEGA group. The lower mean birth weight in the NEGA group compared with that in the EGA group was caused by lower levels of maternal serum thyroid hormones, which function directly or through glucose metabolism.
[Adv Obstet Gynecol 46 (5); 579-587, 1994 (H6.9)]View full abstractDownload PDF (1267K) -
Kozo KUBOTA, Miyako TSUMOTO, Naoki OKADA, Yoichi SHIKADO, Masahiko SHI ...1994 Volume 46 Issue 5 Pages 588-593
Published: September 01, 1994
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSWe evaluated the effect on postoperative pain relief and the incidence of side effects of continuous epidural infusion of buprenorphine 0.2mg (n=25) or 0.4mg/day (n=70), intramuscular injection of 0.2mg b.i.d. (n=12) and other analgesics administered p. r. n. for pain (n=17) for a period of 48 hrs after abdominal simple hysterectomy.
Pain relief was estimated by each patient on a pain score of 0 to 10 (0 : no pain ; 10 : severe pain). Continuous epidural infusion of buprenorphine at the speed of 0.4mg/day after a bolus injection of 0.1mg proved to be most efficacious in giving a stable pain relief and was associated with nausea and/or vomiting in 42.9 % of cases, while 50.0 % of the intramuscular injection group and 17.6 % of the other analgesics group had nausea and/or vomiting. The high incidence (42.9 %) of nausea and/or vomiting in the 0.4mg epidural group declined to 23.0 % after the prophylactic use of Domperidone suppositories, but additional management is necessary to obtain a satisfactory pain relief.
[Adv Obstet Gynecol 46(5) 588-593, 1994 (H6. 9)]View full abstractDownload PDF (944K) -
1994 Volume 46 Issue 5 Pages 625-644
Published: September 01, 1994
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSDownload PDF (4656K) -
1994 Volume 46 Issue 5 Pages 645-662
Published: September 01, 1994
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSDownload PDF (6435K)
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