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Volume 59, Issue 9
Displaying 51-54 of 54 articles from this issue
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Noriyuki Kitamoto, Tetsuya Kimura, Yukio Kito, Kunio Ohmiya, Norihiro ...1995 Volume 59 Issue 9 Pages 1795-1797
Published: September 23, 1995
Released on J-STAGE: February 08, 2008
JOURNAL FREE ACCESSA niaD gene encoding nitrate reductase was isolated from Aspergillus oryzae KBN616 and sequenced. The structural gene comprises 2973bp and 868 amino acids, which showed a high degree of similarity to nitrate reductases from other filamentous fungi. The coding sequence is interrupted by six introns varying in size from 48 to 98bp. The intron positions are all conserved among the niaD genes from A. oryzae, Aspergillus nidulans, and Aspergillus niger. A homologous transformation system was developed for an industrial shoyu koji mold, A. oryzae KBN616, based on the nitrate reductase (niaD) of the nitrate assimilation pathway.View full abstractDownload PDF (536K) -
Sawao Murao, Akiko Nakatani, Nobuaki Kaneda1995 Volume 59 Issue 9 Pages 1798-1800
Published: September 23, 1995
Released on J-STAGE: February 08, 2008
JOURNAL FREE ACCESSA screening test was done to isolate microorganisms producing a highly specific fructokinase. A specific phosphorylative activity toward fructose was found in a cell homogenate of a bacterium, KN-21, newly isolated from a soil sample. The enzyme was isolated from the sonicated cells as a homogeneous preparation. The purified enzyme was composed of two identical subunits (37kDa). The Kms for ATP and fructose were estimated to be 7.0×10-4 M and 1.0×10-3 M, respectively. The enzyme specifically phosphorylated fructose. The use of the enzyme as an analytical reagent was also investigated.View full abstractDownload PDF (469K) -
Hiroshi Ohrui, Toshiaki Waga, Akihiro Ueno, Manabu Okamoto, Hiroko Hor ...1995 Volume 59 Issue 9 Pages 1801-1803
Published: September 23, 1995
Released on J-STAGE: February 08, 2008
JOURNAL FREE ACCESSAdenine reacted with 1, 4-anhydro-3-azido-5-O-benzoyl-3-deoxy2-O-p-toluenesulfon-yl-D-ribitol (4) to unexpectedly give aziridinoadenines (6 and 7), whose structures were determined by X-ray crystallography.View full abstractDownload PDF (346K) -
Akihiro Yamashita, Takumi Yoshizawa, Yusuke Aiura, Priscilla C. Sanche ...1995 Volume 59 Issue 9 Pages 1804-1807
Published: September 23, 1995
Released on J-STAGE: February 08, 2008
JOURNAL FREE ACCESSCorn samples collected from the Philippines, Thailand, and Indonesia were surveyed for the natural occurrence of Fusarium mycotoxins (fumonisins, trichothecenes, and zearalenone) and aflatoxins. Fumonisins B1 and B2 were found in over 50% of corn samples in individual countries, and their co-occurrences with aflatoxins at the incidence of 48% were noted. In addition to these mycotoxins, a trichothecene, nivalenol, and an estrogen, zearalenone, both mycotoxins of Fusarium species, were detected in these Southeast Asian samples. This is the first report on the simultaneous occurrence of two carcinogenic mycotoxins, fumonisins and aflatoxins, together with Fusarium mycotoxins (nivalenol and zearalenone) in corn from Asian tropics.View full abstractDownload PDF (515K)