The sensitivity of
Magnaporthe grisea to Metominostrobin (ORIBRIGHT
®, a QoI fungicide) was examined. Six isolates collected in 1999, prior to the introduction of metominostrobin, were tested by conducting
in vivo assays to establish a baseline for the sensitivity of the fungus. The mean value of the EC
50 (ranging from 0.65 to 11.8 μg/ml) was 3.3 μg/ml. Ninety isolates were collected in 2001–2003 and 56 isolates were subjected to
in vivo assays. EC
50 values ranged from less than 0.1 to 9.4 μg/ml and no significant difference to the baseline was observed. A point mutation at nucleotide position +428 or +387 in the cytochrome
b gene, resulting in the replacement of glycine 143 with alanine (G143A) or of phenylalanine 129 with leucine (F129L) at the amino acid position of cytochrome
b known to be the cause of resistance to QoI, was not observed in any of the 96 field isolates including the six collected in 1999. © Pesticide Science Society of Japan
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