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Volume 20, Issue 1
Displaying 51-58 of 58 articles from this issue
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A MITO, S MURABAYASHI, H MIYAZAKI, Y MORITA, T YUHTA1991 Volume 20 Issue 1 Pages 241-246
Published: February 15, 1991
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSTo study lymphocyte-material interactions is important for the development of the material used for immunomodulation. Therefore, the effect of synthetic polymers on murine lymphocyte were investigated in terms of IL-2 production, IL-2 receptor expression, and proliferation. The lymphocytes were cultured with con A in the presence of four different synthetic polymers, namely; cellulose acetate, polyvinyl alcohl, polyacrylonitril, and polyethylene. In the abesence of sera in culture media, no significant difference in these effects on the lymphocyte functions was observed among the polymers. However, when mouse serum was added, cellulose acetate showed higher IL-2 activity than others, suggesting that serum factor or factors are important for assessing the lymphocyte-material interactions.View full abstractDownload PDF (873K) -
K MATSUDA, H HIRASAWA, T SUGAI, Y OHTAKE, S ODAKE, H SHIGA, N KITAMURA ...1991 Volume 20 Issue 1 Pages 247-253
Published: February 15, 1991
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSThe present study was undertaken to investigate the efficacy of CBP in critically ill patients. During past three years, CBP was performed on 65 patients. For anuric patients continuous hemofiltration (CHF) was effective to make nutritional management with TPN easier and to maintain water, electrolytes and acid-base balance. Another effect of CHF was the removal of causative mediators of organ failure. However CHF was not satisfactory to remove low molecular weight substances, and therefore intermittent hemodialysis (HD) was mandatory. Continuous hemodiafiltration (CHDF) was more effective than intermittent HD combined with CHF in the removal of low molecular weight substances. CHDF could be performed uneventfully under the use of nafamos tat mesilate as anticoagulant even during operation. The study on the reduction rate of creatinine in the relation to filtration rate during CHF and filtration rate+dialy sate flow during CHDF in dicates that it is possible to decrease creatinine level with more than 0.165 l/kg/day of filtrate in CHF and with more than 0.209 l/kg/day of filtrate+dialysate in CHDF. Continuous plasma exchange (CPE) could be performed safely an ffe tively on children and patients with hemodynamic unstableness. Continuous hemoadsorption (CHA) could remove amitryptilhne which widely distributed in organs. From these results we conclude that CBP is a first-choice blood purification in critically ill patients.View full abstractDownload PDF (3061K) -
H. KEINO, T. FUJII, N. TAKAHASHI, M. SUZUKI, A. YAGISHITA, H. SHIMANE, ...1991 Volume 20 Issue 1 Pages 254-257
Published: February 15, 1991
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSWe have developed a system for donor plasmapheresis, which we named Constant Pressure Apheresis System type II (CPAS-II). This system consists of a membrane plasma separator unit and a device which apply the gravitational flow method originally designed for CPAS-I. Whole blood is vacuum-pumped into the blood bag from a donor, and is separated to blood cells and plasma by plasma separator, then the concentrated blood is reinfused to the donor. This separation/reinfusion process is done by pressurizing the blood bag. This system is easy to operate and portable desk top size, therefore it is suitable to be used at various locations including mobile sites and bed side. Ex vivo experiments in canine were performed with 200ml withdrawal volume and 2 cycles apheresis. The results indicated that average N-N time was about 12 minutes and total plasma volume per cycle was about 90ml.View full abstractDownload PDF (489K) -
—SURVEYING AFFINITY-LIGAND FOR CELL SEPARATION—J OKAWARA, M NAGOYA, H YURA, Y YAMAMOTO, M SADO, T AKAIKE1991 Volume 20 Issue 1 Pages 258-262
Published: February 15, 1991
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSIt seems that a technique to separate T cell subsets will be useful for diagnosis and therapy of immunological diseases. For the purpose of developing the technique, we surveyed affinity-ligand for separation of T cell subsets. As the result, it was found that the adsorbent immobilizing Thymopoietin I32-36, which was the biological active site of a thymic hormone, had the selective adhesivity to CD8(+) cells. On the other hand, the adsorbent immobilizing Ricinus Communis Agglutinin, which is one of lectins and can recognize galactose residue, had the selective adhesivity to CD4(+) cells. And these adsorbents showed increased selectivity to T cell subsets under the Ca2+ free condition or at 4°C. T cell subsets which adhered to th adsorbent could be selectively detached by the eluent which contains p-nitrophenyl-β-D-galactopyranoside.View full abstractDownload PDF (4942K) -
S. NAGANUMA, T. SUZUKI, Ps MALCHESKY, Ap KOO, Jd CLOUGH, Y NOSE1991 Volume 20 Issue 1 Pages 263-269
Published: February 15, 1991
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSThe objective of this study is to test the hypothesis that the removal of plasma factors by cryofiltration (CF) will effect changes in cellular immune function and to study the correlation of clinical reponse with cellular immunomodulation by CF in rheumatoid arthritis (RA). Nine RA patients participating in the double-blinded controlled trial of CF underwent either true CF (4) or sham pheresis (5). In addition to clinical assessments, patients mononuclear cell proliferation to mitogens (MP) cultured with normal's or patient's plasma, and normal MP cultured with patient's plasma were studied. The correlation between clinical parameters and MP was evaluated. True CF patients were improved in clinical signs showing active synovitis such as pain, tenderness and swelling of joints compared to sham treatment patients. In 2 of 4 true CF who showed good clinical responses, MP also increased pre to post changes significantly (p<0.05) (average 6336 to 15960 cpm) compared to sham pts (average 6837 to 6552 cpm). In 5 sham patients, both clinical response and MP were not modified by sham pheresis. These data suggested that the positive correlation between clinical improvement and cellular immune response was shown. Assessment of cellular immune parameter may provide a good index for patients selection and therapeutic efficacy in evaluation of treatment modalities.View full abstractDownload PDF (707K) -
K KUKITA, J MEGURO, M YONEKAWA, A KAWAMURA, H FURUI, K KOMAI1991 Volume 20 Issue 1 Pages 270-272
Published: February 15, 1991
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSIn order to improve microcirculation, cryofiltration was applied to 9 cases of multiple myeloma patients who complicated renal failure and/or whose immunoglobulin levels were highly elevated continuously. Diuretic effect was temporally found in one case, and in another, creatinine clearance was improved with the reduction of fibrinogen, immunogrobulin and blood viscosity. In another case, cryofiltration was applied to prevent complications due to microcirculation disturbance after gastrectomy. It was successfully done, and the patient could discharge without major complication. All patients could feel the “lightness of body” after crrofiltration, which seemed one of the effect resulted from improvement of microcirculation. Cryofiltration can improve microcirculation rapidly, then it is useful in cases with the disease which mainly due to microcirculation disturbance.View full abstractDownload PDF (457K) -
K SOEDA, M ODAKA, Y TABATA, M YOSHIDA, K ISONO1991 Volume 20 Issue 1 Pages 273-279
Published: February 15, 1991
Released on J-STAGE: December 02, 2011
JOURNAL FREE ACCESSThe effect of therapeutic DFPP was clinically investigated in drug refractory disorders. The DFPP treatment was performed 248 times (one to three times a week) for 10 patients who had been treated with medical therapy of steroid or immunosuppresive drugs without any improvement in their symptoms. After initiation of the DFPP therapy, neuromuscular disturbances improved excellently and became soon free from DFPP in 6 cases, and got better in 4 cases. Two cases of chrome inflammatory demyelinating polyneuropathy have shown improvement in some of disorders but each of them continued to receive DFPP for 22 months and 16 months, respectively. After DFPP treatment, serum levels of immmunoglobulin were lowered as 41% in IpgG, 59% in IgA and 55% in IgM. These results suggest that DFPP is beneficial and useful for neurological diseases which do not respond well to drug therapy. We recommend Evaflux 3A to be used as a second filter of DFPP because loss of serum protein was minimized and IgG was well eliminated.View full abstractDownload PDF (999K) -
M YAMASHITA, H SAKAMOTO, M UENO, T SHIMOYAMA, S OMOKAMA, Y NOSE1991 Volume 20 Issue 1 Pages 280-283
Published: February 15, 1991
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSPlasmapheresis(PP) has been applied for chronic cholestatic liver diseases in order to reduce cholesterol, bilirubin and bile acid which may be related to symptoms as pruritis, jaundice, and xanthomatous skin lesions. Over 3 to 5 years 299 PPs were employed for 3 patients including 2 patients with primary biliary cirrhosis(PBC) and one patient with primary sclerosing cholangitis(PSC). The symptoms, especially pruritis have been improved. The levels of bilirubin and cholesterol were reduced and well controlled. PP has been shown to be effective in ameliorating clinical symptoms and normalizing laboratory data in PBC and PSC.View full abstractDownload PDF (517K)