RT61 line (derived from
Oryza rufipogon) and BT line (derived from
O, sativa) which are isogenic lines of Taichung 65 indicated similar inheritance of male sterility. In order to determine whether the cytoplasms and fertility restoring genes of these two lines are identical, the following crossing experiments were carried out.
In the cross of the male sterile line/restorer all the F
1 progenies produced fertile pollen in half of the cases while seed fertility was normal regardless of the kind of paternal (or maternal) lines. In the reciprocal crosses of restorer/restorer since all the F
1 plants produced complete fertile pollen and seeds with fertility, there was no difference in the cytoplasms and the fertiliy restoring genes between the two lines.
However, since it was difficult to determine whether the restoring genes were present at the same locus or were closely linked, three-way croses were conducted.
First, F
1s of the male sterile line/restorer were crossed with Taichung 65. In the offsprings there were plants with complete sterile pollen and sterile seeds and plants with fertile pollen in half of the cases and fertile seeds in a ratio of 1 to 1. It was therefore confirmed that the fertility resoring genes were transmitted normally in other cytoplasms.
Thereafter the F
1s of restorer/restorer were pollinated with Taichung 65. Assuming that the fertility restoring genes of these lines were linked and present at a different locus, segregation of male sterile plants would be expected. However, the results indicated that all the F
1 progenies formed normal pollen and seeds. Therefore, the fertility restoring genes of these lines were considered to display the same function for fertility and to be at the same locus.
The above results sugested that the male sterile cytoplasms of the RT line and BT line belonged to the same type for fertility restoration, and the nuclear fertility restoring genes were present at the same locus with the same function.
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