Male sterile cytoplasm and restorer gene of three restorer lines, RT61C, RT98C and RT 102C, in rice were identified and allelic relationships between the restorer genes were studied. Each line was an isogenic line of Taichung 65, cultivar
Oryza sativa, developed by the backcrossing method, with a male sterile cytoplasm and a dominant fertility restorer gene derived from the original line of the wild species of
O. rufipogon. Male sterile lines, RT61A, RT98A and RT102A, which were used as testers were derived from the restorer lines through two backcrossings with Taichung 65, to replace the dominant restorer gene of the restorer lines with the recessive nonrestorer allele of Taichung 65.
Following crosses were carried out: 1. male sterile line/restorer line -to identify the cytoplasm and restorer gene, 2. restorer line/restorer line//Taichung 65 -to detect the allelism of genes among the restorer lines.
The F
1 hybrids showed a high spikelet fertility in both RT61A/RT98C and RT61A/RT102C. All the F
1 plants derived from the crosses of RT98A/RT61C and RT98A/RT102C showed complete sterility. In the cross of RT102A/RT61C the F
1 plants were sterile, while in RT102A/RT98C the F
1 plants were fertile. Thus, the cytoplasms and the restorer genes of the three restorer lines differed from each other.
In the crosses of RT98C/RT61C//Taichung 65, RT98C/RT102C//Taichung 65 and RT102C/RT61C//Taichung 65, the offsprings segregated into a 1 sterile and 1 fertile plant ratio. From these crosses allelism could not be determined. However, in the other crosses, RT61 C/RT98C//Taichung 65, RT61C/RT102C//Taichung 65 and RT102C/RT98C//Taichung 65, all the F
1 plants produced fertile spikelets. The results suggested that the genes of the three restorer lines were located at the same locus.
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