Through successive backcrosses, the cytoplasm and nuclear fertility restorer gene of K98, a line of
Oryza rufipogon, were introduced into Taichung 65,
O. sativa, and a nuclear-substituted line, RT98C, was developed. This line showed a high degree of spikelet fertility, but it produced fertile and sterile plants in the cross with Taichung 65. To analyze the mode of inheritance of the fertility, crossing tests were carried out, and the following results were obtained.
Pollen fertility was controlled by the interaction of the cytoplasm and the single restorer fertility gene (
Rf-rf), but the function of the embryosac was not affected by the cytoplasm and restorer fertility gene. That is, all the pollen grains in the cytoplasm of Taichung 65 were normal regardless of the dominance or recessiveness of the restorer fertility gene they carried. On the other hand, the pollen grains carrying the
Rf gene in the cytoplasm of RT98C were normal, while the pollen grains carrying the
rf gene in this cytoplasm became sterile. The normal cytoplasm of Taichung 65 was designated as (
mfc) and the sterile cytoplasm of RT98C as (
msc) .
When the (
msc)
Rfrf plants were selfed, in all the resulting plants, spikelet fertility was restored, and no sterile plants were observed, suggesting that the pollen grains carrying the
rf gene were not viable. Thus, the male sterility and fertility restoration in this line were controlled by the genotype of the gamete itself but not by that of the zygote, indicating the presence of a gametophytic control of fertility.
Only in the (
msc)
rfrf plants spikelet fertility was not restored, while in the plants with all the other genotypes, viz. (
msc)
RfRf, (
msc)
Rfrf (
mfc)
RfRf (
mfc)
Rfrf and (
mfc)
rfrf spikelet fertility was restored, resulting in a high degree of spikelet fertility. Although pollen grains carrying
rf in (
msc) degenerated regardless of the plant genotype (
Rfrf or
rfrf), they all stained well in a I
2KI solution and showed a normal size.
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