In order to classify male sterile cytoplasms in rice, twelve cytoplasmic male sterile lines (RT2S, RT4S, RT7S, RT18S, RT41S, RT80S, RT81S, RT124S, RT132S, RT134S, RT142S, and RT153S) were crossed with five restoring testers (RT61F, BTF, RT98F, R100F and RT102F) . These lines consisted of Taichung 65's isogenic lines developed through repeated back-crossing of Taichung 65 to wild rice,
Oryza ruffipogon and
O. breviligulata (only RT153S) . The pollen and seed fertilitiy of the F
1 progenies which were derived from crosses between cytoplasmic male sterile lines and restoring testers was evaluated. Based on the results obtained, the cytoplasms of the male sterile lines were classified.
The pollen of the F
1s of RT18S and RT153S which was semi-fertile (50%) was characteristic of a gametophytic cytoplasm. On the other hand, the pollen fertility of all the lines except for these two lines was complete in the cace of hybridization with all the testers, regardless of the seed fertility. Further classification is required for the utilization of seed fertility.
RT18S and RT153S showed a normal seed fertility (more than 90%) in the F
1 for all the testers. RT4S showed a complete sterility for the seeds of F
1 progenies in the crosses with all the testers. The F
1s of RT2S, RT7S, RT41S, RT132S and RT134S displayed a seed sterility (less than 1%) in the crosses with RT61F and BTF, and a higher seed fertility (70-80%) in the crosses with RT98F, RT100F and RT102F. The RT80S and RT124S lines produced seed-sterile F
1 plants in the crosses with RT61F and BTF, a higher fertility in the crosses with RT98F, a lower fertility in the crosses with RT100F, and near-complete sterility in the crosses with RT102F. The RT81S and RT142S lines produced complete sterile seeds in the crosses with RT61F and BTF, but showed a lower seed fertility in the crosses with RT98F and near-complete seed sterility in the crosses with RT100F and RT102F.
The cytoplasms of the twelve cytoplasmic male sterile lines were classified into the following groups: (1) RT18S and RT153S (2) RT4S (3) RT2S, RT7S, RT41S, RT132S and RT134S (4) RT80S and RT124S (5) RT81S and RT142S.
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