Pollinia of
Phalaenopsiswere preserved in nine different organic solvents: acetone, ethyl ether, xylene, toluene, nitro-ethane, nitro-methane, benzene, tert-butyl acetate and n-pentane, and silica gel. Then, the morphological changes in the exine of these preserved materials were observed under a SEM, and the survival rate, seed formation by pollination as well as the formation of protocorms affected by such preservation were evaluated.
1. Only
ca. 40% of the pollinia preserved in nitro-ethane and acetone, respectively for eight months survived.
2. Pollinia kept in silica gel for six months hardly survived.
3. The epidermal pattern of the pollinia preserved for a long period of time with a lower survival rate, was much flatter than that of the fresh ones based on SEM observations.
4. Although pollination with pollinia preserved in tert-butyl acetate or benzene was successful, the enlargement of the capsules was less conspicious than in the case of pollination with normal fresh pollinia.
5. The seeds produced through pollination of the materials preserved in ethyl ether, acetone, tertbutyl acetate readily formed protocorms.
6. This concluded that the survival rate, pollination and seed production of
Phalaenopsispollinia were better when organic solvents such as tert-butyl acetate, benzene and ethyl ether were used for a long period of time than when the pollinia were preserved in silica gel of a low temperature.
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