Intact cells of Staphylococcus aureus, strain AF 121,which had been grown in galactosecontaining broth, hydrolyzed o-nitrophenyl-β-D-galactoside (ONPG), a lactose analog, and released o-nitrophenol (ONP) into the medium. The activity was totally lost by treating the cells with toluene, but it was restored when phosphoenolpyruvate (PEP) was supplemented to the medium. Therefore, this method can be conveniently used for demonstrating the requirement of PEP for the ONPG-hydrolysis reaction in this organism. The activities obtained varied with conditions of the treatment. The highest activity was observed when the cells were treated with 6μl of toluene per ml for 30 min at 37℃.
Mr. R. K. Zeigler has found some interesting results about the asymptotic distribution of the sample mean and the sample median. In this paper I deal with the simultaneous characteristic function of the product of the two random variables. Let the probabity density function, the mean, standard deviation and the median of the distribution be denoted by f(x), m, σ and θ respectively. Let the mean deviation from the sample median be denoted by M and put [numerical formula], [numerical formula], where [numerical formula]. The characteristic function ψ(t) of the simultaneous distribution of u and υ will be shown as follows; [numerical formula], where 0(t)→0,n→∞
1. The sensitivity of thin-layer chromatography is measured for the several lichen substances. 2. The minimum quantity of each lichen substance for chromatographic detection is as follows : 0.0025μg (lecanoric acid) ; 0.005μg (lobaric acid) ; 0.01μg (zeorin) ; 0.16μg (usnic acid) ; 0.3μg (vulpinic acid). 3. By usual microchemical extraction, 4.6% of dry weight of the thallus (Parmelia tinctorum Nyl.) can be obtained as the crude extract of lichen substances. The results for the other three lichens are as follows : Anzia colpodes (0.42%), Cladia aggregata (0.9%), Usnea diffracta (1.25%). In contrast, by using usual chemical extractors in the chemical laboratory, the crude extract (1.8g) of lichen substances was obtained from the extraction of the thalli (21g) of Usnea diffracta by acetone (8.6%). 4. The amount of the lichen substances can be estimated in some extent by the spot area on the chromatogram. 5. In the microchemical method, usually 2.5-5μg lichen substances are spotted on chromatograms ; therefore, if only one lichen substance is present in the acetone extract, the lichen substance can be detected without exception. However, if two or more lichen substances are present in the acetone extract, small amount of the lichen substance (i.e., less than 0.3μg in vulpinic acid, and less than 0.0025μg in lecanoric acid) may not be detected by the chromatography.
It is a most serious problem for a hemiplegic patient in convalescence, how to minimize the lost functions (i.e. how to regain the possibly good ADL). Among the problems to be thought for the lost functions, it is a most difficult and important one, how to prevent the equino-varus deformity of a parahysed foot to expect the restoration from inability to walk. For the purpose, many sorts of braces and adequate programs of the exercise had been prescribed and performed during hospitalization. As show in many investigations, however, we know the many patients who lost the regained functions of walking at all, by falling into disuse of their braces, soon after discharge from hospitals, because of the heaviness and some other troubles of the given braces themaelves. To resolve such problems, we attempted to devise a new shoe horn brace from "Below Knee Polypropylen Orthoses" (as "the semimanufactured goods" from USMC Co., USA), which could be remodelled by heats and obtained statisfactory results, both in easy application and in good walking pattern. Its advantages could be thought to be as follows; 1) Very highter weight (about 120 g.) as compared with usual shoe type SLB (about 1,000 g). 2) easiness to put on and off. 3) to be permitted to walk in the houses of old Japanese fashion (i.e. to walk on "Tatami"), and easiness to put a shoe directly over it, to go out. 4) to be cosmetically acceptable. 5) easiness of washing to keep it always clean. Further, we can saved troubles, such as the cast modifications and several-times checking. Also, we can obtain the materials easily as "the semimanufactured goods". Remained problems, we must follow up, are the tolerance of the materials and the influences upon the ankle ROM in long time usages.
Studies reported here were undertaken to examine the distribution of protein in rice grains of several varieties produced in Kochi Prefecture. The acrolein-Schiff reaction on tissue sections for the rice grain of 8 varieties revealed its high specificity for proteins. These variant characteristics of the distribution of protein bodies in the rice grain were represented by the following three types. (1) In the first type, protein was distributed very richly in the aleuron layer and the outer layer of endosperm, and a little in the central part of endosperm cell walls. (2) In the second type, protein was distributed very poorly in the central part of endosperm. (3) In the third type, protein was widely distributed from the outer layer of endosperm to the inner layer.
In performing periodic medical examination per annum, the result is, as a general rule, presented in the from of numerical indication, i.e. figures. Such an indication in figures seems not to serve us as an informative guide to grasping the characteristics of respective school as a whole and of individuals. The present research has been made with the object of learning the actual condition which physical constitution is measured and classified in Kochi prefecture. In addition thereto, the date thus conglomerated may be of great use in furthering their physical strenght as well as physique. Involved in the research are : 134 elementary schools, 94 junior high schools, 29 senior high schools and 23 part-time high schools in Kochi prefecture. They, having ceased going througt a process of under-development, corpulence, and wide breast, now tent toward sound development firly balanced. It is positively recognized, none the less, that some features seen in physique of juveniles in elementary schools and/or senior high schools still remain to be further ameliorated. These findings lead me to believe that acitive measures should be taken hereinafter for improving their nourishment concurrently with encouraging physical discipline.
In the present report, the fine structure of the sporewall of the Japanese Cyrtomium ; C. vittatum H. Chr., C. tachiroanum (Luerss.) C. Chr. and a from of C. falcatum (L. F.) Pr., were observed by both light-and electron-microscopes. The results are summarized in tabl 1. The spore-wall of those species mentioned above were not clearly distinguished by each other with light-microscopic observations, but their fine structures were able to be distinguished with electron-microscopic observations.