1. By using the scanning electron microscope, we observed fine structures of the dentinal carious lesion in human permanent teeth (C_4). 2. The following successional carious lesion can be observed with the scanning electron microscope. When dead tracts are formed in the dentinal part, the calicification occur in dentinal tubules (P1. 2a). Then, the decalicification follows to form hollow dentinal tubules (Pl. 1a). Finally, both discoloration and disintegration of carious dentines (P1. 2b) were observed.

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In the previous paper, we have already clarified by the analysis of several elements, potassium, Sodium, and total nitrogen contained in the chicken (minced meat) in a various conditions that boiling simply the solids in an amount of water is effective for the preparation of Low-Potassium Diets. In this experiments, we chose chicken (minced meat) again and fish meat (spanish mackeral) as solids and analyzed quantitatively the components, sodium, potassium, phosphorous, calcium, and protein contained in each samples dealt in several times and temperatures by warming in water. For the aim of lowering the dissolution of calcium and protein from the solid into water, the abrupt conditions were controlled to warm the solids at 80 ℃ and for 10 minutes. Furthermore, some analysis of minerals and protein contained in 3rd Degrees Diets for the patient of kidney diseases over 3 days and each diets dealt by boiling in water for 10 minutes lead us to declare that the dealt diet exhibit 30% decrease of total quantity of potassium in a day comparing with that of usual diet.

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The animals were observed daily for 14 days following administration of the test material, permasorb. Gross autopsy examination showed the stomach to be filled with firm tough, rubbery, white test material, but the gross appearance of the stomach, intestines, and other viscera did not reveal any obvious cause.

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The toxic effects of permasorb on mice, after the reagent had been mixed into its food over 4 weeks, were studied. Examinations of appetite (food intake), excrements, general appearances or behaviours of mice lead us define that no significant signs oral toxicity was recognized on mice. Anatomical examination also exhibited no change of the outlooks of internal organs of mice from normal ones.

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