The large ribosomal subunits of rat liver contain small amount of minor RNA components which migrate through polyacrylamide gels between the two major rRNAs, and some 28 SrRNA molecules contain hidden nicks which can be revealed by heat treatment. The minor RNA components were isolated, labeled in vitro with ^125I, and hybridized with nucleolar DNA from rat liver. The hybridization-competition experiments with 28 S RNA established that the minor RNA components and 28 S rRNA have a common DNA origin. When RNA components were isolated from rats that had received [^14C] orotate and [^3H] orotate, 1 week and 16h, respectively, before sacrifice, the ^14C : ^3H ratio was the highest in the minor components, followed by the nicked RNA, and the intact 28 S rRNA showed the lowest ratio. We also found that partial hepatectomy decreases and the actinomycin D-treatment increases the amount of the minor RNA components as well as the nicked 28 S RNA. From these observations, we conclude that the initial step of degradation of 28 S rRNA in vivo is the nick formation and that the nicked RNA subsequently gives rise to the minor components.
The degradation rates of the rat liver microsomal alkaline ribonuclease were examined in vivo by using cycloheximide. The half-life if the ribonuclease was about 6 hours. Our result indicates that the half-life of the ribonuclease is disagree with that of microsomal ribonuclease obtained by Ferdinand.
Distribution of phosphoenolpyruvate : lactose phosphotranseferase system (lactose-PTS) in strains of staphylococci isolated from human oral mucosae, was studied. The activity was determined by measuring hydrolysis of o-nitrophenyl-β-D-galactoside in toluene-treated cells supplemented with phosphoenolpyruvate as the phosphate donor. The lactose-PTS was distributed in both Staphylococcus aureus and epidermidis strains.
We found histone hydrolytic activity in rat liver lysosomes at high specific activity. The enzyme having histone hydrolytic activity was purified 26 fold from rat liver homogenate and separated from the other cathepsins in rat liver lysosomes by chromatography on DEAE-cellulose and Sephadex G-200. The partially purified enzyme has hydrolytic activities to both histone and casein. We tried to separate histone hydrolytic activity from casein hydrolytic activity by column chromatography and isoelectric focusing electrophoresis. However, we failed to separate these activities. The column chromatography profiles are similar fto histone and casein hydrolytic activities. In present enzyme histone and casein hydrolytic activities show optimum activity at pH 5.0 and pH 6.0,respectively. These activities are activated by dithiothreitol and are inhibited by iodoacetic acid and L-Leupeptin at the same extent. Histone hydrolytic activity has two isoelectric points at pH 4.09 and pH 6.04 which are exactly same as those of casein hydrolytic activity. Molecular weight of the enzyme is about 37000 by gel filtration. Judging from above results we may conclude that enzyme having both activities is a new enzyme different from other cathepsins and histone hydrolases.
The isolation of Streptococci was performed from dental plaque in 59 dental hygienic students to investigate the intraoral distribution of caries-inducing Streptococcus mutans. Streptococci could be easily isolated from dental plaque at high rate in anaerobic system using Gas Pak on the Chapman's Mitis salivarius agar plates at 37℃ for 24 hr and the isolation appeared as in grey-white circular colonies varying in size from 0.5 to 1.0 mm. The isolation rate from pits and fissures, predilection areas of dental caries, were a little higher, however, this work did not completely explaine the relationship Streptococcus mutans to dental caries and the isolation procedure must be more examined. The isolated Streptococci were morphologically identical with reference strain of Streptococcus mutans on electron microscopic study.
This study investigates the sportsman's will movement experimentally, and the purpose of this study is to put this result to practical use in the actual games. For the first step, about the will movement called risktaking I investigated closing and escaping to reward and punishment and prudence by playing dart from psychological side. There was a man who wasn't afraid of "Risk" even in risk and dared to challenge it or on the other hand a man who was afraid of risk and selected the safe movement. Selection of will was different by each character. The difference between their characters was also investigated by the character test and I tried to look through sportsman's character.
I summarised my general grasp of Fracture of the femoral neck, on the basis of the pro gnosis overhauling 95 cases of this fracture through the treatment at TANAKA ORTHOPEDIC HOSPITAL for the lest 10 years. (1) The after-effects were found on the 81 cases, made up as follws ; Intracapsular Fractures…35 cases (42%) Extracapsular Fractures…46 cases (68%) Regarding the cause : The major cause for these fractures was a fall, having most caused ; 58 cases (72%) (2) Regarding the age and the sex distinction : The age of being injured was most in their seventies, on the average, at the age of 68.4; In sexualizing, males were in 29 cases. Females were in 52 cases. Females were by far more than males. (3) Regarding the days of hospital treatment : 46 cases (56%) were in less that 2 months. 35 cases (43%) were in more than 2 months. The longest 28 months, the shortest 2 days. (4) Regarding another trouble : About 50% of all cases had some another anamnesis. As regards Union, among 51 cases which follow-up (by X-ray photography) could be taken to, 23 cases (45%) had some another trouble. (5) Regarding the activities of daily living ; especially ambulation ; It was possible for the 83% cases to be ambulant, and the 90% cases could take care of themselves at home (go to stool).
The Oike Sun Nursing Home in Kobe admits old people who are more than sixty-five years old, and who at the same time always need constant nursing care because of their physical or mental condition. For these reasons it is difficult to give them sufficient care in their own homes. Lately the physical therapy performed at the nursing home has been queationed as to its necessity and various problems concerning the therapy have been discussed. Since 1974 the physical therapy service has been given to 41 patients at our nursing home. We have case histories of 23 patients that were given the therapy service for more than one year. Records have been kept on the degree of their functional disorder and on the progress of their recovery from the disorder under the remedy of the therapy service. Our case histories indicate that 1. In most cases the functional disorder does not appeare in their eyesight, hearing or speaking ability or their eating habits, but in their motor ability, that is in transferring to or from a wheel chair, changing clothes, excereting-especially in bathing because of the movement it requires and the bath room fixtures that are not suitable to their disability. 2. The speed of recovery varies and the degree of the improvement seems to be very low three months after the remedy was begun. It required more than six months at least before we could see evident recovery. 3. If the staff of the home could not give motivation to the patients and if the patients could not give their full attention to their physical movements in all aspects of their daily life, the effects of the physical therapy alone were very limited.
We should like to describe two points in this article. One thing is the biomechanics of an osteoarthritic hip that is related by Bombelli who makes use of Fischer's theory and Pawells' theory. The other is the therapeutic experience of Bombelli's valgus-extension osteotomy. We experienced ten cases in connection with the operation of Bombelli's valgus-extension osteotomy in Kitahyogo-Seikeigeka center. The object of this osteotomy is to reduce the pressure on the head of the fumer, to exploit the hydraulic mechanism, and to strech even more the superior zone of the joint capsule, the ligamentum teres and their synovial membrans. The roof osteophyte is formed by streching these tissues. The osteotomy is indicated in an osteoarthritis of the hio with a passive movement of about thirty degrees of flexion and fifteen degrees of adduction. The patient may walk with two crutches in seven days sfter operation, and to aboid full weight bearing for about six months. When he walks, he must set one foot directly in front of the other. We compared the function of the patients with that before the operations, when they discharged the hospital, at the mean time of sixteen weeks after the operation. It is recognized to reduce the activities of daily living for the reduced range of motion of the hip, and to delay the strengthening of gluteus medius and gluteus maximus. It is advisable to convince the patient to wait for six months or a year.