A highly specific antibody to LH-RF was generated in female rabbits by frequent immunization with synthetic LH-RF-BSA conjugate.In vitro incubation gave 35-40% binding of ¹²⁵I-LH-RF and a linear dose-response relationship against cold LH-RF (from 3-2500 pg) using 1: 1500 dilutions of the antiserum. This antiserum showed no marked immunocross-reactivity with rat LH and FSH, Substance P, oxytocin, lysine and arginine vasopressin or synthetic TRF compared with synthetic LH-RF. These substances were less than 0.03% displacement of LH-RF under these conditions. The precipitin line disappeared after absorption by BSA, though the antiserum reacted with the LH-RF-BSA conjugate on the Ouchterlony agar plate test. Intravenous injections of 0.8 ml of this antiserum to 4-day cycling female rats on the day of proestrus blocked ovulation on the following day. In these rats, LH surge on the evening of proestrus was blocked, but LH was maintained at basal levels. Two to five ng of LH-RF was detected in adult mature rat hypothalamus. Subcellular fractions were radioimmunoasayed for LH-RF The synaptosomal fraction contained 5% LH-RF as compared. with whole hypothalamic tissue (47.6ng in 17 rats). For immunohistochemical demonstrations, the antibody was applied to rats and mice hypothalami by new and direct immunoenzyme techniques. Adult Wistar-Imamichi rats and ICR mice were decapitated and the hypothalami were prefixed with 2% paraformaldehyde and freeze-dried paraffin or cryostat sections were postfixed with Zamboni's or PLP solutions (found to be the most satisfactory). LH-RF was detected in the cytoplasm of some cell bodies close to the ependymal lining in the lower part of the arcuate nucleus. Other specific LH-RF neurons were observed in the medial preoptic, prechiasmatic, anterior hypothalamic, ventromedial hypothalamic and mammilloinfundibular (or ventrolateral premammillary)nuclei. LH-RF was also localized in the organum vas culosum of the lamina terminalis (OVLT). Ependymal tanycytes, however, were never immunoreactive upon absorption of the antiserum with rat or mouse liver powder prior to the immunohistochemical incubation on the rat or mouse brain tissue, respectively, (indirect method). The other penventricular organs were not studied.

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Pretreatment with cadmiu m is reported to protect animals, to some extent, from acute toxicity of a following administration of a higher dose of cadmium itself. On the other hand, it is still unknown as to whether or not the teratogenicity and/or the fetolethality of cadmium are reduced by such pretreatment with cadmium. Based on a report that administrations of cadmium sulfate to pregnant mice on their 7th day of gestation caused exencephaly and death of their fetuses, the authors investigated possible prevention of teratogenic and fetolethal effects of cadmium by pretreatment with a smaller dose of cadmium. On day 6 of gestation, a low dose (0.5 mg/kg or 1 mg/kg)of cadmium fulfate was administered intraperitoneally (ip) or subcutaneously (sc) to pregnant JCL: ICR mice, and on the next day a high dose (5 mg/kg) of the same compound was administered ip. Dams were sacrificed on day 17 of gestation and con. ceptuses were examined. A dose of 0.5 mg/kg of cadmium ip failed to show a protective effect against teratogenicity, but 1 mg/kg sc or 1 mg/kg ip was effective. However, induction of exencephaly was not completely blocked by these pretreatments. On the other hand, the protective effect against fetolethality was remarkable and fetal death rates were significantly lowered by overall pretreatment to control values.

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1. The present study was made on the anterior, the middle and the posterior superior alveolar arteries in 25 adult dogs by means of the acryl plastic injection method.
2. The posterior super i or alveolar artery: It generally arises from the infraorbital and its main stream turns to the zygomatic glandular branch. The dental and alveolar branches of it spread on the maxillary tuber to supply all roots of M₂, often also the distobuccal and lingual roots of M1. Rarely the artery arises from the common trunk between the sphenopalatine and the major palatine or the middle superior alveolar or sphenopalatine or the minor palatine.
3. The middle superior alveolar arteries: They,5-13 in number, arise from both the infraorbital and the anterior superior alveolar and supply P₁, P₂, P₃, P₄ and M₁. According to the origin and location of them, they are grouped into four as follows: The arteries, thickest, arising from the infraorbital proximal to the entrance of the same named canal supply all roots of M1 and the distobuccal root of P₄; the arteries arising in the canal supply the mesiobuccal root of P₄; the arteries arising from the anterior superior alveolar proximal to the entrance of the same named canal supply the lingual root of P₄ and the mesiobuccal root of M₁, rarely the lingual and distobuccal roots of M1; the arteries arising in the canal supply the mesial root of P₃ an d all roots of P₁ and P₂.
4. The anterior superior alveolar artery: It arises from the infraorbital in the same named canal, and enters the anterior superior alveolar canal with the satellite nerve arching above the canine root apex. It supplies the canine and the interalveolar septum between C and I₃ occasionally also I₃ and I₂.
5. Distributing arteries of t he upper teeth are not only the anterior, the middle and the posterior superior alveolar arteries but also other arterial resources locating in the vicinity of the teeth such as the sphenopalatine, the gingival branch of the infraorbital, the minor and major palatine, the malar and the dorsal nasal artery of the infraorbital.
6. Each of the upper teeth is supplied with the following arterial resources:
I₁, mainly with the major pala tine, rarely the sphenopalatine, but not with the anterior superior alveolar in any cases.
I₂, mainly with the major palatine, sometimes the anterior superior alveolar, rarely the sphenopalatine.
I₃, with the sphe nopalatine, or the anterior superior alveolar more frequently than in case of I₂, but the frequency of the major palatine is reduced.
C, with the canine branch of the anterior superior alveolar in all examples observed.
P₁, P₂ and the mesial root of P₃, with the middle superior alveolar arising from the anterior superior alveolar of the posterior lateral nasal artery of the sphenopalatine, sometimes with both of them for a single root.
The distal root of P₃ and the mesiobuccal and lingual roots of P₄, with the middle superior alveolar arising from the anterior superior alveolar and the same named artery arising from the infraorbital, sometimes the gingival branch of it, or with all of them for a single root. (The distal root of P₃ and other teeth locating posterior to it were not supplied with the posterior lateral nasal artery of the sphenopalatine. )The distobuccal root of P₄, with the middle superior alveolar of the infraorbital in all examples observed, rarely the malar artery.
The mesiobuccal root of M₁ and M₂, with the middle supe r ior alveolar of the infraorbital and the posterior superior alveolar.
The lingual and distobuccal root of M₂, with the posterior superior alveolar.

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