巻号一覧
58 巻, 2 号
選択された号の論文の4件中1～4を表示しています
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YASUKAZU NAGATO, TSUYUKA KUSHIDA, HIROSHI KUSHIDA1981 年 58 巻 2 号 p. 69-79
発行日: 1981年
公開日: 2012/09/24
ジャーナル フリーA mixture of formaldehyde and glutaraldehyde as a fixative in conjunction with GMA-Quetol 523 embedding readily permits the preservation of semi-thin sections for routine use in combined light and electron microscopy. Combined 4%formaldehyde-2% glutaraldehyde fixation gives excellent preservation, and general and histochemical staining performed at the light microscopic level provides satisfactory results in the 0.5 to 1.0μm thickness range. Employing the above fixative and GMA-Quetol 523 sections in this range, nuclear and cytoplasmic components such as the nucleoli, mitochondria, Golgi apparatus and cytoplasmic granules, were clarified with various basic and acid dyes, especially hematoxylin and eosin. DNA, glycogen and other polysaccharides were also sufficiently well preserved to study their localization in 0.5-1.0μm sections.
The effectiveness of glutaraldehyde, formaldehyde, formaldehyde-glutaraldehyde, periodate-lysine-paraformaldehyde, Bouin's and Zenker's solution on GMA-Quetol 523embedded tissues was compared.
Formaldehyde-glutaraldehyde mixtures promise to be a very useful fixative for use in GMA-Quetol 523 embedded and are suitable for the semi-thin sections necessary in combined light and electron microscopic studies. The results were both visually pleasing and ideal for photomicrography.抄録全体を表示PDF形式でダウンロード (3572K) -
HIROSHI MUTO, NORIO OZEKI, IKUO YOSHIOKA1981 年 58 巻 2 号 p. 81-97
発行日: 1981年
公開日: 2012/09/24
ジャーナル フリーStudies were made of a hair from the scalp of a young healthy man. The hair had suddenly changed from black to gray during formation. The following results were obtained.
1. Melanin granules were little recognized in the cuticle of the gray part near the hair root, but they were observed in the cortex of the same hair.
2. The hair medulla was observed in the black part of the scalp hair tip and in the gray part near the hair root. The hair medulla in the gray part especially was seen to include destroyed hollows.
3. A close relation was found to exist between the formation of the hair and number of cuticle layers. The hair medulla remained when the speed of formation of the hair was more rapid. That is to say, the cuticle layers decreased in number when the hair formation was rapid.
4. Black hairs or melanin granules did not develop from the hair root whose hair medulla was destroyed.
5. The proximal end of the cuticle cells towards the center of the hair revealed a shape like the teeth of a comb. That is to say, the end of the cells had several cell drocesses.抄録全体を表示PDF形式でダウンロード (3932K) -
MARI ASADA-KUBOTA, SHINSUKE KANAMURA1981 年 58 巻 2 号 p. 99-113
発行日: 1981年
公開日: 2012/09/24
ジャーナル フリーDifferences in the ultrastructure between periportal and centrilobular hepatocytes were studied quantitatively for newborn,5-day-old,10-day-old and adult mice. In newborn animals there were no differences between periportal and centrilobular cells in the area and number (per unit area of the cytoplasm in crosssection)of the mitochondria, smooth endoplasmic reticulum, rough endoplasmic reticulum, lysosomes, peroxisomes and lipid droplets, and in the shape of the mitochondria. In 5- and 10-day-old animals, however, differences were seen in the area of the mitochondria and smooth endoplasmic reticulum, and in the shape of the mitochondria. Adult hepatocytes show additional differences in the number of the mitochondria and peroxisomes, and in the area and number of the lysosomes. Thus, in general, the ultrastructural heterogeneity among hepatocytes, evident in adult animals, is not present in newborn animals but appears during postnatal development.抄録全体を表示PDF形式でダウンロード (4415K) -
TOSHIO TERASHIMA1981 年 58 巻 2 号 p. 115-135
発行日: 1981年
公開日: 2012/09/24
ジャーナル フリーThe collateral branching of crossed lateral pyramidal tract (PT) fibers was studied in the rhesus monkey, employing the method of semi-thin sectioning of Epon-812 blocks to enable precise counting of the degenerated myelinated fibers.
The PT which projects to the motor nuclei innervating the contralateral upper limb muscle was electrocoagulated within the posterior limb of the internal capsule. The figures for the degenerated myelinated fibers of the crossed lateral PT showed increases at C4 and C8, or T8 from the pyramid down to the spinal cord. This suggests that the parent fibers of the crossed lateral PT give off collaterals within the white matter of the lateral funiculus.抄録全体を表示PDF形式でダウンロード (3982K)
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