0.5 ml of 2.5 % alloxan solution was injected intraperitoneally to a mouse, and the cytochemical examinations of liver with the passage of time thereafter showed the following results:
1) Following the injection of alloxan, alkaline phosphatase shows generally a temporary decrease in activity, and then it recovers. The decrease in activity is particulary distinct in the central region of the hepatic lobule. The activity of the interlobular bile duct is intense, but no difference of activity is observable between each group. The stellate cell, even in the region neighbouring the central vein also, gives so intense an activity that the presence of the nucleus is difficult to recognize, being covered by the deep coloration of the cytoplasm, and the activity is reduced slightly in the one hour, in the two hours and in the four hours groups.
2) The acid phosphatase activity increases with the passage of time. The intensification is remarkable particularly in the nuclei of the hepatic cell and of the stellate cell. In the six hours group, the activity indicates the time intensity as that of the control group.
3) The PAS reaction decreases generally the stainability with time after the injection. The stellate cell shows an intensification of the stainability following the injection, but there is no remarkable differen ce of intensity observable between the groups, sacrificed in different time lengths after the injection. A cytochemical investigation on the mouse liver after alloxan injection 363
4) The stainability produced by the mercuric chloride-BPB metho d increases with the passage of time after the injection.
I am greatly indebted to Prof. T. Taniguchi for his kind and invaluable criticism. This work has been done under the project of Instructor M. Ihnuma.
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