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Volume 26, Issue 3
Displaying 1-21 of 21 articles from this issue
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[in Japanese], [in Japanese], [in Japanese]1985 Volume 26 Issue 3 Pages 315-331
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSDownload PDF (1471K)
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—The Significance of Their Levels and Existence of Complexes as Diagnostic Indicators—Takashi TSUDA, Hisatomo HAYASHI, Naokazu TSURUMI, Masaki KURODA, Yutak ...1985 Volume 26 Issue 3 Pages 332-340
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSAntithrombin III (AT III) and α2-plasmin inhibitor (α2PI) which are regarded as the major modulators of DIC were studied in 40 patients with DIC. The patients were classified into the acute promyelocytic leukemia (APL) group and the group of other underlying diseases which were also evaluated according to the absence or the presence of liver damage as a complication. However, there were no patients with APL suffered from liver damage.
Functional activities and concentrations of AT III and α2PI were measured by chromogenic methods and immunological methods, respectively. The existence of their complexes were also determined by crossed immunoelectrophoresis (CIE).
Higher rates of abnormalities in functional activities of both AT III (100%) and α2PI (75%) were observed when compared to their respective concentrations.
AT III-protease complexes and plasmin-α2PI complexes were positive in 46% (6/13) and 40% (6/15) of the patients with DIC, respectively. It was noteworthy that AT III-protease complexes were detected in 60% of the group with liver damage and especially in 75% of the group with severe liver damage in which functional activities and concentrations of AT III were below 50% each. This suggests that an analysis of AT III-protease complexes by CIE would be useful in distinguishing consumption coagulopathy with impared synthesis of coagulation factors from coagulopathy caused by hyposynthesis of such factors in liver damage.View full abstractDownload PDF (700K) -
Keiji OKUBO, Yoshinobu MATSUO, M. Mitsuo YOKOYAMA1985 Volume 26 Issue 3 Pages 341-348
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSWe had previously reported that a monoclonal antibody KOLT-1 (KOLT-1 MoAb), produced by hybridoma clone, was specific for human T lymphocytes.
We have further characterized KOLT-1 MoAb and compared it with OKT4 and Leu3a MoAb using laser flow cytometry system. The percentages of KOLT-1+ lymphocytes in controls and patients with adult T-cell leukemia (ATL) were similar to that of OKT4+ cells and Leu3a+ cells.
The results indicated that the T lymphocyte subset recognized by KOLT-1 MoAb was identical with that detected by OKT4 while the antigen or epitope was not identical.
KOLT-1 was highly reactive with ATL cells but had less than 20% reactivity with other leukemic cells and infectious mononucleosis cells which were analysed for cell membrane markers. Therefore KOLT-1 MoAb should be useful as an aid in the diagnosis of ATL.View full abstractDownload PDF (572K) -
Masako KATOH, Tatsuo SHIRAI, Masanori UMEDA, Ryozo YAMAGUCHI, Haruo KA ...1985 Volume 26 Issue 3 Pages 349-356
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSTo investigate whether LTB4 also stimulates the monocyte (MON) chemotactic response, human MONs and polymorphonuclear leukocytes (PMNs) were tested for both the chemotactic and the chemokinetic responses for LTB4, by the agarose plate method, and compared with 3 other chemotactic factors; Zymosan activated serum (ZAS), bacterial culture filtrate (BCF) and formyl-methionyl-phenylalanine (FMP). 1) LTB4 stimulated both chemotaxis and chemokinesis of PMNs: Chemotactic Index (C.T.I.=chemotaxis/random mobility (RM)): FMP 2.83>ZAS 2.22>LTB4 2.20>BCF 1.33; Chemokinetic Index (C.K.I.=chemokinesis/RM): LTB4 1.88, FMP 1.78. 2) LTB4 revealed to stimulate a potent chemotaxis for MONs. C.T.I. 4h (18h): FMP 2.25 (1.75)>ZAS 1.62 (1.61)>LTB4 1.59 (1.58)>BCF 1.19 (1.29). 3) But LTB4 did not stimulate MON chemokinesis at the concentration of 20 ng/ml. C.K.I. 4h (18h): LTB4 0.71 (0.76), FMP n.d. (1.61). As the migration speed is much slower than PMN, the MON chemotactic deactivation may have occured during the long incubation period. Conclusions: LTB4 revealed to have a potent chemotactic effect for MONs, which play an important role in the late stage of inflammation, and in immune reaction system as antigen presenting cell, regulatory cell and effector cell as transformed into macrophages.View full abstractDownload PDF (744K) -
Takashi HANADA, Tsukasa ABE1985 Volume 26 Issue 3 Pages 357-361
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSIn order to detect suppressor lymphocytes to hematopoietic stem cells, CFU-E colony growth was compared before and after pre-treatment of bone marrow cells with anti-lymphocyte globulin (ALG) and complement in 17 patients with aplastic anemia. Peripheral blood T lymphocytes which suppressed allogeneic CFU-E colony growth were found in 7 cases. In 3 out of these 7 cases, CFU-E colony growth from patients' own marrow cells was enhanced significantly by pre-treatment with ALG and complement, but in the remaining 4 cases no significant enhancement was found. ALG therapy was given 3 cases in whom pre-treatment of bone marrow cells with ALG and complement in vitro enhanced CFU-E colony growth. This therapy was effective in only 2 cases. It is suggested that the results of in vitro tests can not predict the effectiveness of ALG therapy in vivo.View full abstractDownload PDF (390K) -
Kazumi SAMPI, Toshiyuki TAKAGI, Yasunobu KURAISHI1985 Volume 26 Issue 3 Pages 362-366
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSThree participating institutions of Saitama Cancer Center, Chiba Cancer Center and Jikei University School of Medicine studied about on accuracy of lymphangiography (LG) and computed tomography (CT) of abdomen in patients with malignant lymphoma with particular attention to the retroperitoneal area between January 1982 and April 1984.
Eighty-five procedures of LG and CT of abdomen among 81 patients were performed. Each procedure was done within one month and patients who were though to be not influenced by cancer chemotherapy were selected. Both modalities were in full agreement in 67 cases (78.8%). Forty-seven cases were diagnosed as having a negative retroperitoneum and pelvis by both modalities, whereas 15 cases were called positive by both studies. Five patients were interpreted as equivocal by both CT and LG. Interpretations differed in 18 cases (21.2%). Of these, three patients had positive LG, but CT demonstrated negative findings.
In conclusion, as far as a diagnosis of retroperitoneal lymph nodes is concerned, CT of abdomen was considered to replace the lymphangiography.View full abstractDownload PDF (485K) -
(1) Platelet Derived Granulocyte Aggregating FactorFumio IMANAKA, Kingo FUJIMURA, Atushi KURAMOTO1985 Volume 26 Issue 3 Pages 367-373
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSRecent studies demonstrated that large numbers of platelet-granulocyte thrombi are lodged in the pulmonary microvasculature in adult respiratory distress syndrome (ARDS), suggesting an important aspect of intravascular granulocyte-platelet interactions.
We investigated the effects of platelet components on granulocyte aggregation in vitro. Granulocyte fraction was separated using a Percoll density gradient method. Platelet homogenates were prepared by sonication. Granulocyte aggregometry was performed in a standard platelet aggregometer.
Platelet homogenate strongly aggregated granulocytes, as N-formylmethionyl-leucyl-phenylalanine (FMLP) or zymosan activated serum (ZAS) did. On the other hand, supernatant of thrombin induced platelet aggregation also aggregated granulocytes. This granulocyte aggregating activity of platelets was heat labile and Ca++ dependent but non-dialyzable. This activity was not inhibited by lectin competitive inhibitors and protamine sulfate that competes platelet derived growth factor (PDGF) but was inhibited by hydrocortisone. Platelet derived granulocyte aggregating factor might be released by thrombin induced activation but physicochemical characterization and its localization are still unidentified, other than PDGF, PF4 The important role for granulocyte-platelet interactions in ARDS or other pathological status such as inflammation and arterial injury was stressed.View full abstractDownload PDF (492K) -
I. Cytogenetic Studies of the Colonies Obtained from Leukemic PatientsToshiyuki ISHIBASHI, Hideo KIMURA, Rokuo ABE, Tsutomu SHICHISHIMA, Shi ...1985 Volume 26 Issue 3 Pages 374-380
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSBone marrow cultures were performed in 3 cases of acute non-lymphocytic leukemia (ANLL) and a case of blastic crisis of chronic myelocytic leukemia (CML-BC), using the method described by Minden.
Morphological and cytochemical studies including peroxidase, α-naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase showed that the cells from the colonies were similar to the leukemic cells before culture.
To investigate the leukemic nature of the colonies, cytogenetic analyses were done in a single colony and in pooled colonies in 3 patients with ANLL and one with CML-BC who had marker chromosome in short term culture of the marrow. The banding analyses of the chromosomes from the colonies revealed the same abnormalities in high percentage. The cytogenetic analyses in the two cases in remission revealed that granulocytic-macrophage colonies (CFU-GM) and erythrocytic (BFU-E) colonis had normal karyotype.
It was concluded that most of the colonies consisted of the undifferentiated cells in the culture system by Minden were derived from “leukemic” stem cells.View full abstractDownload PDF (1193K) -
Masahiro FUJIWARA, Yoshiharu NAKAMORI, Masaru URUSHIYAMA, Satoru KOYAM ...1985 Volume 26 Issue 3 Pages 381-386
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSWe studied on the prognosis and in vitro CFU-C-derived colony formation of 19 patients with aplastic anemia. In the assay of CFU-C, the patients with severe aplastic anemia had very low colonies and clusters. Anabolic steroids were effective on 57% of patients, and were less effective on patients with severe disease than on patients with mild disease. There was no difference between oxymetholone and mepitiostane on the effect or the adverse effect. Anabolic steroids were more effective on patients having higher CFU-C colony formation. Immunosuppressive therapy was effective on a part of patients, whose CFU-C increased early after the therapy.View full abstractDownload PDF (425K) -
Yoshitatsu SEI, Keiji OKUBO, Kaori YASUDA, Akinori HARA, M. Mitsuo YOK ...1985 Volume 26 Issue 3 Pages 387-391
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSWe studied 9 healthy adults who have shown to be predicted a defective feature of the OKT4 antigenic epitope on the membrane of peripheral blood lymphocytes. Lymphocyte membrane markers of the subjects were analysed by flow cytometer utilizing various monoclonal antibodies (OKT4, Leu3a and KOLT-1) reactive with OKT4+ cells.
The results appeared to indicate that the lymphocytes lacking reactivity with OKT4 while Leu3a and KOLT-1 are normally reactive. One of them, who is a 33 yr-old healthy female, and her family were examined. The parents and sister had a normal proportion of OKT4+ cells but fluorescence intensity indicating mean channel number of these cells was decreased. The present data suggested that the lack of OKT4+ antigen on their lymphocytes is due to hereditary abnormalities of the antigenic epitope.View full abstractDownload PDF (345K)
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—Its Possible Influences on the Hemolysis in Heat Burn Patient and on the Pretransfusion Cross Match Test—Susumu OKUBO, Tomoko ISHIDA, Kojiro YASUNAGA1985 Volume 26 Issue 3 Pages 392-398
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSa) A three years old male of blood type A Rh(0), suffering from heat burn (30% of his whole body surface, grade II-III) developed anemia with signs of low grade hemolysis from the 3rd day after transfusion of heat-treated human plasma protein (PPF, 5,500ml in total), albumin (800 ml) and immunoglobulin preparations (40 gr.), and the direct Coombs' test turned out positive. The hemolysis could be attributed to the anti-A antibody of IgG type, which was detected in the eluate from his red cells. As to the origin of the antiboay, these plasma preparations infused to him were suspected.
b) Anti-erythrocyte antibodies in PPF, albumin and immunoglobulin preparations in market: Anti-A and Anti-B antibodies of IgG type were detected in every lot of these preparations examined. The highest titers were 1: 512 for PPF and albumin, 1: 128 for immunoglobulin preparations by the antiglobulin test. And also, anti-D antibody was detected in two of the immunoglobulin lots.
Although no papers reporting on the anti-erythrocyte antibodies found in the PPF, albumin and immunoglobulin preparations and on the hemolysis caused by them could be found in literatures, these results obtained might suggest that the transfusion of a large amount of these plasma preparations can induce hemolysis, especially in low body weight patients, and that the antibodies originating from these preparations may become one of the causes yielding positive Coombs' test in the control tests using self red cells.View full abstractDownload PDF (505K) -
Tetsunori FUNABIKI, Hideki SASAKI, Yoshitaka KOISO, Koichirou IKUTA, K ...1985 Volume 26 Issue 3 Pages 399-404
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSA 7-year-old boy with chronic myelogenous leukemia complained of severe headache and vomiting twelve months after the diagnosis. A large number of atypical blastoid cells were found in the cerebrospinal fluid. The number of the blast cells in the bone marrow moderately increased, while peripheral blood examination showed characteristics of chronic phase. After intrathecal injection of anti-leukemic agents, leukemic cells in CSF disappeared and the patient became free of all his complaints. We could not define whether the transformation initially occured in the bone marrow or in the central nervous system.
Tachypnea, polydipsia and polyuria appeared eight months later, and laboratory findings suggested renal tubular acidosis (RTA). Intravenous pyelogram and ultrasonic echogram revealed enlargement of the right kidney, and renal hypofunction was shown by renogram. Three weeks after the initiation of the vincristine and prednisolone therapy, acidosis and hypokalemia were improved, and the size and function of the kidney became within normal limits. The course suggested that RTA was also due to infiltration of leukemic cells.View full abstractDownload PDF (763K) -
Koji SUGAI, Kazuo MORI, Sozo SUZUKI, Yasuyuki AKUTSU, Masaaki ISHIKAWA ...1985 Volume 26 Issue 3 Pages 405-410
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSHemarthrosis and intramuscular hemorrhage are most frequently found in hemophiliacs. But, though less frequently, differential diagnosis of acute abdomen in hemophiliacs is very important and life-saving. The authors report a rare case of submucosal hemorrhages of jejunum in a patient with hemophilia B.
A 26-year-old hemophiliac was admitted to Tohoku University Hospital complaining of nausea and right lower quadrant pain with rebound tenderness, but he didn't develop leukocytosis. For about 20 years, he often experienced the same symptoms and was treated conservatively every tine. In the diagnosis of acute appendicitis, the patient was operated on under careful hemostatic control. At exploration, appendix vermiformis was found intact and further exploration was performed to result in the elucidation of submucosal hemorrhages of jejunum causing intestinal obstruction. Partial resection of jejunum was done without either serious bleeding nor signs of DIC syndrome. The postoperative clinical course was uneventful. The total prothrombin complex concentrate (PCC) used was 30,000 units. The patient was discharged 20 days after admission.
The literatures containing submucosal hemorrhage of gastro-intestinal tract in hemophiliacs are reviewed.View full abstractDownload PDF (448K) -
Johji INAZAWA, Junichi EDAGAWA, Yuji OKAMOTO, Kazuhiro NISHIDA, Shoich ...1985 Volume 26 Issue 3 Pages 411-416
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSA 25-yr-old Japanese female in 18-wk gestation was admitted on July 9, 1982 with a 2-wk history of general malaise and low-grade fever. On admission, peripheral blood examination revealed anemia (Hb 8.7 g/dl) and leukocytosis (WBC 20,300/cmm) with 52% leukemic cells. These leukemic cells were identified as lymphoblasts with T-cell phenotype. A diagnosis of T-cell acute lymphoblastic leukemia was made. Chest X-ray and echocardiogram showed pericardial effusion, in which a number of lymphoblasts were found. After received therapeutic artificial abortion, she was treated with an induction regimen consisting of daunomycin, adriamycin, vincristine, cyclophosphamide. L-asparaginase, cytocine arabinoside and prednisolone as well as intrapericardial administration of methotrexate. After 6 courses of treatment, complete remission was achieved on her 52th hospital day. She is alive without any signs of relapse. Prior to therapy, cytogenetic analysis revealed a consistent chromosome rearrangement; 46, XX, t (5; 12) (q35; q13).View full abstractDownload PDF (748K) -
Mitsuyuki FUKUDA, Akira MIURA, Hiroki MASUDA1985 Volume 26 Issue 3 Pages 417-422
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSA 52-year-old man with chronic myeloid leukemia, for whom elective splenectomy had performed in chronic phase in order to prolong the survival period, developed thrombocytosis, more than 250×104/mm3, with resultant angina-like symptoms and syncopal attacks between 3 and 6 years after splenectomy. Treatment with ACNU and periodic thrombopheresis had partial success. Seven years after splenectomy, he admitted because of weakness, jaundice, ascites, edema and shortness of breath. Examination resulted in the discovery of hepatic insufficiency, earry stage of blast crisis, ARDS and prerenal azothemia, and he died in a few days. Autopsy revealed esophageal varices and marked cell infiltraion into many organs, especially in the liver, kidney and lung. There were vigorous cell infiltratin and extramedullary hematopoiesis in the hepatic sinusoid with many small noduls containing monotonous blastoid cells leading to atrophy, degeneration and loss of hepatic cells.
Only one case of chronic myeloid leukemia, which converted blast phase by hepatic insufficiency as to the first manifestation, has been descrived.View full abstractDownload PDF (550K) -
Shiro NAKAYAMA, Kenichi NAGAI, Teruo YOSHIDA, Hiroki YABE1985 Volume 26 Issue 3 Pages 423-428
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSDespite improvement in complete remission rate by intensive induction therapy, a considerable number of patient with adult acute non-lymphocytic leukemia (ANLL) still fail to enter complete remission because of death from infection or hemorrhage. Therefore, two patients with ANLL were treated with a modified two-step method. The first step was 7 day-course of BH-AC 300 mg, ACM 20 mg, 6MP 100 mg, and prednisolone (BH—AC·AMP). After a pause of 10 days, the patients were given 2 courses as the second step consisting of low doses of Ara-C (15 mg/12h) for 13-22 days with a 12-15 day rest period. The duration of the first step was relatively short compared with that of the conventional BH-AC·AMP therapy, and by this course leukemic cells were partially killed without serious marrow impairment. Residual leukemic cells were affected slowly by 2 courses of the second step of therapy resulting in disappearance of marrow blasts about 2 months after the start of small doses of Ara-C without further progress of cytopenia. Thus, complete remissions were obtained without noticeable complications about 3 months after diagnosis in the 2 patients. After achieving complete remission, consolidation therapy of 5 days with the same agents as at the first step was given. Since this method of therapy had minimal cytotoxicity on the normal hematopoietic cells, requirements of red cell and platelet replacement were decreased, and granulocyte transfusions were not necessary because serious infections common in conventional BH-AC·AMP therapy had not been observed. It is concluded that this method may be worth trying for its safety as remission inducer in patients with ANLL.View full abstractDownload PDF (450K) -
Isao SANADA, Seiko YAMAMOTO, Misa OGATA, Makoto KAWAKITA, Fumio KAWANO ...1985 Volume 26 Issue 3 Pages 429-435
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSA 73-year-old female patient was admitted because of splenomegaly and leukocytosis. Physical examination revealed slight anemia, hepatosplenomegaly and a few spontaneous ecchymoses in the extremities. Peripheral blood examination showed leukocytosis with mature neutrophils and anemia: RBC 289×104/cmm, Hb 9.4 g/dl, WBC 19,800/cmm and platelet count 14.5×104/cmm. 86.5% mature neutrophils, rare immature neutrophils and extremely high levels of neutrophil alkaline phosphatase were observed in the smear of peripheral blood. Bone marrow biopsy revealed generally increased connective tissue with loss of fat and increased megakaryocytes and mature neutrophils. Cytogenetic study showed no Ph1 chromosome in 2 days culture of unstimulated peripheral blood. The underlying diseases, which caused a leukemoid reaction, were not detected. These findings supported the diagnosis of chronic neutrophilic leukemia. However, chromosomal study showed Ph1 chromosome after long-term culture of bone marrow and peripheral blood cells. This may indicate that by long-term culture, even if not in the short-term culture, Ph1 chromosome could be detected in chronic neutrophilic leukemia.View full abstractDownload PDF (636K) -
Haruo HORIKOSHI, Fumio SAKAMOTO, Ichiro OHKI, Tetsuo KUWABARA, Kazuo D ...1985 Volume 26 Issue 3 Pages 436-441
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSA 33-year-old houswife was diagnosed to have acute nonlymphocytic leukemia in April, 1980. Complete remission was achieved by the DCV (daunorubicin, cytarabine, and vincristine) therapy. Meningeal leukemia developed in hematological remission in May, 1981 which was successfully treated by intrathecal methotrexate. In July, 1982 a painful abdominal mass, extending from the pelvis to the umbilicus, was found, and leukemic infiltration was confirmed in the uterus and ovaries by CT scanning and biopsy. Irradiation was effective to reduce the size of the tumors and to eradicate leukemic cells as shown later in the resected uterus and ovaries. In September of the same year, paraplegia occurred below the 7th thoracic level, which was proved by myelography and CT scanning to result from a spinal tumor.
The neurological signs were improved by irradiation.
The first hematological relapse occurred in October, 1983 in association with pleural effusion containing numerous leukemic blasts. Bone marrow remission was again achieved and pleural effusion disappeared by the BHAC-AMP (enocitabine, aclarubicin, 6-mercaptopurine, and prednisolone) therapy. Currently, the patient is doing well four years and four months after the onset of her disease.View full abstractDownload PDF (868K) -
Masayuki OHNISHI, Satoru KOYAMA, Kenji KISHI, Yoshiaki MORIYAMA, Shoji ...1985 Volume 26 Issue 3 Pages 442-447
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSA 61-year-old man of Fusarium infection during treatment for acute lymphocytic leukemia (L2 of FAB classification) is described.
Leukemic blasts were 16% in the peripheral blood and 86% in the bone marrow on his admission day, and were resistant to combination chemotherapy; leukemic blasts were remained 30% in the bone marrow on the 22nd hospital day. He was complicated with Pseudomonas aeruginosa septicemia, and was stopped cytotoxic drugs and treated with antibiotics, gammaglobulins and granulocyte transfusions. He had erythematous skin lesions on the scalp and in the face and the extremities at that time. These lesions were changed into a blackish necrosis with scab and healed with a detachment. Cultures of these lesions grew two colonies which were composed of F. solani and F. oxysporum. Histological findings revealed septate hyphae in the epidermis and the vessels of the dermis. Amphotericin B infusion and oral 5-flucytosine were continued until a granulocyte count in the peripheral blood increased, because high fever continued.
He had the remission on the 31st hospital day, but relapsed after 5 months. He died of cerebral hemorrhage after 5 months from a diagnosis.View full abstractDownload PDF (796K) -
Toshihiko IWAMOTO, Tatsuhiko KYO, Shinzo TACHIKAWA, Takashi KINO, Hide ...1985 Volume 26 Issue 3 Pages 448-454
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSA case of prolymphocytic leukemia categorized by Galton et al is reported.
The patient, a 73-year-old man, was admitted to our hospital because of a sense of fullness and general fatigue. Physical examination revealed anemic conjunctivae, marked splenomegaly and a few swollen lymph nodes of less than soy-bean size. The peripheral blood showed RBC 284×104/cmm, Hb 7.8 g/dl, platelets 9.8×104/cmm, and WBC 142,700/cmm with 91% of lymphoid cells. These cells had a pale bluish-gray cytoplasm, and the nucleus was relatively large with a single prominent nucleolus and coaser nuclear chromatin structure than that of a lymphoblast. On bone marrow aspiration and lymph node biopsy proliferation of these cells was noted. Under electron microscopy these cells had a narrow cytoplasm containing a few mitochondria and short rough endoplasmic reticulum with some microvilli. The nucleus showed peripheral chromatin condensation with one large prominent nucleolus. TdT activity was negative. Surface marker analysis of these lymphocytes revealed characteristics of B cells with markedly elevated surface IgG and M. These findings suggested B cell prolymphocytic leukemia. Vincristin and prednisolone (VP), therefore, were administered, but pleuritis was complicated followed by death 10 months after the onset.
Chromosome analysis demonstrated hypodiploidy of 43XY with abnormal karyotypes of C and D groups.
Prolymphocytic leukemia is characterized morphologically by marked increases in such prolymphocytes with a single prominent nucleolus. This disease is considered to be a distinct clinical entity from the viewpoint of some specific chromosome changes.View full abstractDownload PDF (883K) -
Hidemi YANO, Masao KATO, Akira ISHIGURO, Eizaburo ISHII, Kohki AOYAMA, ...1985 Volume 26 Issue 3 Pages 455-460
Published: 1985
Released on J-STAGE: February 02, 2009
JOURNAL RESTRICTED ACCESSWe report a 5-year-old girl with acute lymphoblastic leukemia (ALL) in the second remission who was treated with syngeneic bone marrow transplantation (BMT), followed by the maintenance chemotherapy. She was given marrow transplant from her identical twin after cyclophosphamide (60 mg/kg×2) and fractionated total body irradiation (2Gy×5). Fifteen weeks later, she was placed on the maintenance chemotherapy with 6MP, methotrexate, prednisolone and vincristine.
Pancytopenia and immunologic abnormalities such as imbalance of T lymphocyte subsets and suppressed in vitro immunoglobulin production were promptly recovered. She has survived for more than twelve months in complete remission.View full abstractDownload PDF (438K)
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