All issues
Volume 38, Issue 1
Displaying 1-7 of 7 articles from this issue
- |<
- <
- 1
- >
- >|
-
[in Japanese]1986 Volume 38 Issue 1 Pages 1-15
Published: January 01, 1986
Released on J-STAGE: October 11, 2011
JOURNAL FREE ACCESSDownload PDF (1779K) -
Hidenori YOKOYAMA, Jun TAKEYAMA, Kaichiro YAMAMOTO, Masanori IKEDA, Ki ...1986 Volume 38 Issue 1 Pages 17-25
Published: January 01, 1986
Released on J-STAGE: October 11, 2011
JOURNAL FREE ACCESSIrradiation-caused changes in L-1210 cellular morphology and the effect of irradiation on cell Kinetics were investigated. With respect to morphological changes, as exposure dose was raised, both cellular and nuclear diameters increased. Cells became giant, multinuclear, and finally, ghost cells. Thus, the progression to cell death was suggested. Nuclear DNA histograms disclosed accumulation to G2M phase as exposure dose was increased. G2 block was suggested in terms of both cellular morphology and nuclear DNA histogram. At the same time the amounts of BrdU and DNA were measured. One result of irradiation was decreased S phase fraction;that is, DNA synthetic rate. As exposure dose increased, accumulation was observed to a point which seemed to be late S phase and G2M phase, until at last DNA synthesis was discontinued, The process to discontinuance was revealed.View full abstractDownload PDF (2409K) -
—Evaluation of Estrogen Production in Neoplastic Trophoblasts—Masaaki KOBAYASHI, Takeshi MARUO, Matsuto MOCHIZUKI1986 Volume 38 Issue 1 Pages 27-37
Published: January 01, 1986
Released on J-STAGE: October 11, 2011
JOURNAL FREE ACCESSIn order to assess the ability of neoplastic trophoblasts to produce estrogen, dehydroepiandrosterone sulfate (DHAS) loading test was performed. Fifty mg of DHAS was administered intravenously to normal healthy subjects and patients with trophoblastic diseases. Serum concentrations of estradiol (E2) were measured at 0, 15, 30, 60, 180 and 240 min after DHAS administration. E2 conversion ratio (E2CR) accumulated for 60 min after the injection was calculated.
In patients with hydatidiform mole, invasive mole, or choriocarcinoma, serum E2 levels rapidly increased after the injection, reaching the peak at 30-60 min. E2CR calculated in patients with these trophoblastic diseases was markedly higher as compared to that in normal healthy subjects. In the course of effective treatment, the E2CR in those patients decreased to normal E2CR levels in healthy subjects in a similar manner to declining pattern of serum hCG levels. There was a significant correlation between E2CR and serum hCG level not only in patients with hydatidiform mole or invasive mole but also in patients with choriocarcinoma. When the correlationship between E2CR and serum hCG levels in patients with choriocarcinoma was compared to that in patients with hydatidiform mole or invasive mole, the conversion rate of DHAS to E2 in patients with choriocarcinoma was found to be smaller than that in patients with hydatidiform mole or invasive mole.
These results suggest that choriocarcinoma trophoblasts remain the enzyme activities of sulfatase, 3β-HSD and aromatase which are required for the conversion of DHAS to E2 and that the capability of estrogen production in choriocarcinoma is lower than that in hydatidiform mole and invasive mole. Thus, it may be possible that analytical comparison of E2CR to serum hCG levels in patients with trophoblastic diseases enables to speculate the character of neoplastic trophoblasts.View full abstractDownload PDF (1196K) -
Alsaku MORI, Yoichi FUKUDA, Juri NODA, Shinichi YANO, Hisashi TSUKUI, ...1986 Volume 38 Issue 1 Pages 39-48
Published: January 01, 1986
Released on J-STAGE: October 11, 2011
JOURNAL FREE ACCESSThe influence of water quality on mouse in vitro fertilization (IVF) and embryonic development was studied by using five types of water preparations, characterized by the electrical conductivity (μs/cm), ion concentration (ppb) and the pattern of organic compounds eluted from a column (μBondapak) by high performance liquid chromatography to detect the kind of substances that were inhibitory. The results obtained with mouse IVF were satisfactory (66-68%) in the Biggers-Whitten Whittingham's medium (BWW) prepared with Milli-Q pure water (0.06μs) and water distilled three times (0.8μs), but they were unsatisfactory (5-20%) in the medium prepared with the other three types of water. When 8 cell embryos were cultured, most of them could reach the stage of expanded blastocyst (89-95%) with considerably high rates of subsequent blastocyst hatching (45-54%) in BWW prepared with water distilled one (1.2μs), water distilled three times and Milli-Q pure water. Essentially, the same results were obtained (58-69%) under the same conditions in the embryos cultured from the 2 cell stage, but a difference was noted in the hatching process. Fourty-eight percent of the expanded blastocysts hatched in the Milli-Q BWW, whereas only 15-24% did in the other conditions. This difference was clear when the embryos were cultured from the single cell stage. Tap water (190μs) and deionized water (0.6μs) were deleterious throughout all experiments perfomed.
The elution profile of organic compounds from the column showed both a high quality of Milli-Q pure water and unexpected contaminations possibly derived from glassware or silicone tubing during the preparation of glass-distilled water.
Pure water preparations seem to be essential for IVF and subsequent embryo culture, and hatching seems to be an appropriate indicator to assess the environmental conditions and developmental potential of the embryos cultured in vitro.View full abstractDownload PDF (4010K) -
Yasuo GOTO, Mariko NAITO, Yoichi NODA, Hiroshi OZASA, Takahide MORI1986 Volume 38 Issue 1 Pages 49-52
Published: January 01, 1986
Released on J-STAGE: October 11, 2011
JOURNAL FREE ACCESSA functioning uterus may be present in 5 to 10% of the patients with congenital absence of the vagina. The proper treatment for these patients depends on accurate pre-operative diagnosis on whether or not a cervix is present, because the mode of operation should be selected according to the type of abnormality. Ultrasonography or X-ray CT scan will not always be useful to answer this question. We encountered a 15-year-old female with cyclic lower abdominal pain and the failure of onset of menstruation. The external genitalia were normal except for the absence of the vaginal opening.
Ultrasonography and X-ray CT scan revealed the presence of both uterine corpus and hemato colpos, but no definite findings were obtained on the integrity of the cervix. By using NMR-CT scan, we found a hematocolpos, properly developed uterine cervix and corpus above it. We treated the patient by preserving the uterine function and the potential for pregnancy in the future. NMR-CT scan seems more useful in some cases for diagnosis of pelvic mass than ultrasonography and X-ray CT scan.View full abstractDownload PDF (1815K) -
Masanori YAMASHITA, Yoichi NODA, Takahide MORI1986 Volume 38 Issue 1 Pages 53-58
Published: January 01, 1986
Released on J-STAGE: October 11, 2011
JOURNAL FREE ACCESSEndocervial specimens from pregnant women were examined for Chlamydia trachomatis by using the Micro Track Direct Specimen Test. Of 395 pregnant women who visited the out-patient clinic of Maizuru Municipal Hospital, 17 women (4.3%) were infected with Chlamydia trachomatis, 9 of whom were treated with Erythromycin (250mg, orally, four times a day) at 35 week's gestation. After 14 days of treatment, all patients had no evidence of Chlamydia trachomatis infection. No infection was also observed in newborn infants delivered from these 9 women as examined by throat swabbing at discharge. In contrast, one newborn infant among the 5 delivered from 5 patients with chlamydial infection without treatment developed a cough and tachypnea 4 weeks after delivery. Direct Specimen Test gave positive results for specimens from throat swabbing, chest X-ray photograph showed a characteristic reticulo-granular pattern and IgG (1, 540mg/dl) and IgM (285mg/dl) concentrations were elevated, but there was no evidence of viral infection. The newborn infant was diagnosed as having chlamydial pneumonia. After 3 weeks treatment with erythromycin (30-40mg/kg/day) the patient fully recovered.
The Micro Trak Direct Specimen Test was considered to be useful for detection of chlamydial infection because of its simplicity in examination procedure. The incidence of 4.3% (17/395) in maternal infection does not seem not remarkably high, but taking into account the high incidence of the infant infection (one out of five), pregnant women should be examined for chlamydial infection during the course of pregnancy.View full abstractDownload PDF (3278K) -
A Case Report of Congenital, Giant HydroureterSatoshi NATSUYAMA, Yoichi NODA, Hiroshi OZASA, Yoshikazu MASAI, Ikuo K ...1986 Volume 38 Issue 1 Pages 59-62
Published: January 01, 1986
Released on J-STAGE: October 11, 2011
JOURNAL FREE ACCESSA 14-year-old girl was admitted to our hospital with complaints of general malaise and lower abdominal distension. Physical examination revealed a large (8×10cm), tense elastic mass occupying the pelvic cavity and lower abdomen. Peripheral blood analysis, serum biochemistry and tumor-marker data showed no abnormalities, but intravenous pyelogram demonstrated a left non-visualizing kidney. Ultrasonography of the tumor showed a large cystic mass without solid portions, which replaced the uterus anteriorly and to the right. The tumor was suspected to be an ovarian cyst. Exploratory laparotomy revealed the bilateral ovaries to be normal, and there was a retroperitoneal mass continuous with the bladder and the left contracted kidney, which was diagnosed as a left giant hydroureter. The left ureter was removed with left-nephrectomy.
Our case of giant hydroureter was considered to be due to congenital stenosis of vesico ureteral junction, and was classified as mechanically obstructive megaloureter. Although congenial, giant hydroureter is a rare disease, it is a clinically important entity in the differential diagnosis of lower abdominal tumor occurring in young women.View full abstractDownload PDF (4149K)
- |<
- <
- 1
- >
- >|