Human blood was incubated in the water bath at various temperature (050°C) for various time (5 90min), to experiment the effects of temperature on APTT, PT, fibrinogen, activities of clotting and fibrinolytic factors, platelete aggregation and its morphology. The results were as follows.
1. APTT and PT prolonged significantly at the border temperature, 43°C
2. Fibrinogen was clottable at 045°C, and unclottable at 50°C. But the changes of fibrin clot strand was observed under scaning at 43 °C. Prothrombin activity decreased at 50 °C, and factor X at 45 °C.
3. Plasminogen activity increased at 43 and 45°C, but decreased at 50 °C. Prekallikrein showed almost no change throughout 050 °C.
4. Platelets lost their aggregative function at 43°C using collagen, at 40°C using ADP. Simultaneously morphorogical change was observed at 43°C under scanning electron microscope.
From these results, we concluded that 43°C might be critical very important temperature in blood coagulation and fibrinolitic system as well as cell-survival in hyperthermia.
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