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Jiale FU, Shinichi KAKUDA, Feng PAN, Shuhei HOSHIKA, Shihchun TING, An ...
2013 年 32 巻 2 号 p.
203-211
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
The purpose of this study was to evaluate the short-term µTBS (Micro-tensile bond strength) and microscopic (SEM and TEM) observation of four recent adhesives. One adhesive was an experimental step-less 1-step system (LLB-2, Tokuyama Dental), which is an all-in-one system without the light-curing step in the application process. The other two were self-adhering light-cured flowable composite resin systems FLD (Fusio Liquid Dentin, Pentron Clinical Technologies) and VF (Vertise Flow Dental Restorative Materials, Kerr Corporation), which combine all the bonding steps together. A 2-step self-etching system MG (Clearfil MegaBond, Kuarary Medical) was employed as the control group in this study. The µTBS of MG was the highest (79.0 MPa) followed by that of LLB-2 (63.1 MPa), FLD (23.6 MPa), and VF (13.1 MPa). The microscopic observations showed that MG and LLB-2 had an approximately 20 µm and 5 µm adhesive layer respectively, without bubble or gap-formation at the resin-dentin interface, which were found in FLD and VF.
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Hitomi MITA, Yuichi KITASAKO, Tomohiro TAKAGAKI, Alireza SADR, Junji T ...
2013 年 32 巻 2 号 p.
212-218
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
This study aimed to evaluate effectiveness of Phosphoryl-Oligosaccharides of Calcium (POs-Ca) added to apple juice on enamel erosion. Five juices were prepared by adding 0%, 0.5%, 1%, 1.5% or 2% POs-Ca to commercial apple juice, and subjected to Visual Analogue Scale (VAS) taste evaluation and pH and calcium/inorganic-phosphates analyses. To evaluate erosion, polished bovine enamel blocks were immersed in each juice for 5 or 60 min (
n=20). Enamel surface loss (SL) and roughness (Ra) were also analyzed. VAS indicated acceptable taste for juices containing up to 1% POs-Ca. POs-Ca addition resulted in increased pH (3.61-3.88), calcium (0.95-25.10 mM), and inorganic-phosphate (1.77-20.44 mM). After 5 min, 0% juice resulted in significant erosion (
p<0.05). However even after 60 min, no significant increase was found in Ra and SL compared to water (control) for 1.5-2% juices (
p>0.05). Addition of 1-1.5% POs-Ca could significantly reduce enamel erosion by apple juice maintaining an acceptable taste.
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Takafumi EGOSHI, Yohsuke TAIRA, Kohyoh SOENO, Takashi SAWASE
2013 年 32 巻 2 号 p.
219-227
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
This study investigated the effects of surface treatments on the bond strength of a resin composite to a commercially pure titanium. The bonding surfaces of all titanium specimens were ground with 1,000-grit silicon carbide paper and then subjected to one or more of these surface treatments: sandblasting with alumina (sand), etching with 45wt% H
2SO
4 and 15wt% HCl (SH-etchant) at 70°C for 10 min, and/or phosphate primer (MDP-primer) application. Specimens not subjected to any surface treatment were used as controls. After resin composite veneer placement and 24-h water immersion, the shear bond strengths of the specimens in descending order were: sand/SH-etchant/MDP-primer, sand/SH-etchant/no primer, no sand/SH-etchant/MDP-primer, sand/no etch/MDP-primer, no sand/SH-etchant/no primer, sand/no etch/no primer, no sand/no etch/MDP-primer, no sand/no etch/no primer. Scanning electron microscope observations revealed that sandblasting and SH-etchant created many micro- and nanoscale cavities on the titanium surface. Results showed that a combined use of sandblasting, SH-etchant, and MDP-primer application had a cooperative effect on titanium bonding.
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Fernanda Calabró CALHEIROS, Carmem S. Costa PFEIFER, Ludmila Lourenço ...
2013 年 32 巻 2 号 p.
228-232
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
This study evaluated the influence of specimen dimensions and storage medium and temperature on the flexural properties of one dental resin composite. The material utilized throughout the study was Clearfil AP-X. Eighty specimens were built with dimensions of either 10×2×2 mm or 25×2×2 mm, stored dry or in distilled water, at 25 or 37ºC, for 24 h. Flexural strength and modulus were tested in three-point bending. Results were evaluated with three-way ANOVA and Tukey's test (α=0.05). Statistical significance was observed for all three factors: temperature (37ºC=190.8 MPa, 25ºC=165.3 MPa;
p<0.01), storage medium (water=167.7 MPa, Dry=188.4 MPa;
p<0.01) and specimen dimensions (10×2×2 mm=197.9 MPa, 25×2×2 mm=158.2 MPa;
p<0.01). None of the interactions were significant. Within the limitations of this study, it can be concluded that factors such as specimen dimensions, storage medium and temperature significantly affect flexural strength results, which invalidates comparisons of results from studies that use different methodologies.
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Shizuka YAMADA, Yuu YOSHIZAWA, Atsushi KAWAKUBO, Takeshi IKEDA, Kajiro ...
2013 年 32 巻 2 号 p.
233-240
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
This study was designed to investigate the biological effects of fish collagen peptide (FCP) on human osteoblasts. Human osteoblasts were treated with 0.1% FCP, which was the optimal concentration confirmed by the increase in alkaline phosphatase activity. After one, three, five and seven days of culture, the number of FCP-treated cells increased significantly compared with untreated cells. In a real-time PCR analysis, the expression of osteocalcin, osteopontin, BMP-2 and integrin β3 mRNAs in FCP-treated cells showed increases compared with untreated cells after three days of culture. After seven days of culture, the expression levels of osteopontin and integrin β3 were still higher in the FCP-treated cells than in untreated cells. The production of osteocalcin, osteopontin and integrin β3 proteins in FCP-treated cells also showed increases after seven days of culture. Furthermore, FCP accelerated matrix mineralization in the cultures. The present study indicates the potential utility of FCP as a biomaterial.
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Sachiko UTAKA, Syozi NAKASHIMA, Alireza SADR, Masaomi IKEDA, Toru NIKA ...
2013 年 32 巻 2 号 p.
241-247
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
This study aimed to evaluate the potential of a new device (Cariotester) for monitoring of incipient carious lesion remineralization in root dentin by topical fluoride
in vitro. Demineralized bovine dentin specimens were treated by fluoride solutions (APF or neutral NaF) and remineralized for 4 weeks. Cariotester was used to measure penetration depth (CTR depth) of the indenter into the de- and remineralized specimen surface. The specimens were assessed by transverse microradiography (TMR) to determine lesion parameters (depth: LD, mineral loss: ΔZ). Pearson's correlation analysis showed an overall significant relationship between CTR depth and both TMR parameters. CTR depth appeared to distinguish the positive effect that topical fluoride application had on the remineralization of the outer zone of dentin lesions. Cariotester had the potential to serve as a quantitative tool for monitoring of incipient carious lesion remineralization in root dentin.
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Jianfeng ZHOU, Xu YANG, Li CHEN, Xiaoqiang LIU, Lin MA, Jianguo TAN
2013 年 32 巻 2 号 p.
248-255
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
We evaluated whether the pre-treatment of radicular dentin by ED Primer containing different concentrations of chlorhexidine can improve the bond durability of fiber post to radicular dentin. Experimental ED primers containing different concentrations of chlorhexidine (0%, 0.5% and 1.0%) were prepared. Thirty extracted maxillary anterior teeth were divided into 3 groups, each group corresponding to different chlorhexidine concentrations. Fiber posts were cemented in endodontically treated teeth with experimental ED primers and Panavia F. The bonded teeth were transversally sectioned into six slices and then were processed for thin slice push-out test 24 h later or after 18-months water storage. Eighteen-month storage resulted in significant bond strength reduction of all groups (
p<0.05). The bond strength reduction of 1.0% group was significantly lower than that of control group and 0.5% group (
p<0.05). In conclusion, the incorporation of 1.0% chlorhexidine into ED primer can extend the bond longevity of fiber post to radicular dentin.
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Akira SAITO, Emiko SAITO, Yoshinori KUBOKI, Mariko KIMURA, Toshinori N ...
2013 年 32 巻 2 号 p.
256-262
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
The aim of this study was to investigate the effect of tunnel structured β-tricalcium phosphate (β-TCP) on the regenerative potential of basic fibroblast growth factor-2 (bFGF-2) in class III furcation defects in dogs. The furcations of 30 mandibular premolar teeth received: 1) 0.3% bFGF-2 solution in conjunction with β-TCP; 2) 0.3% bFGF-2 solution; and 3) no implant material (Control group). The dogs were sacrificed 8 weeks post-surgery, and healing was evaluated histologically. New bone formation was significantly greater in the bFGF-2/β-TCP group compared to the bFGF-2 solution and Control groups (
p<0.01). New cementum formation in the bFGF-2/β-TCP and bFGF-2 solution groups was significantly greater than that in the Control group (
p<0.01). These findings suggested that bFGF-2 alone enhances connective tissue attachment in a manner similar to the combination of bFGF-2 and β-TCP. Furthermore, this combination enhances bone formation up to the fornix in class III furcation defects.
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Paulo Henrique Perlatti D'ALPINO, Natália Coelho de FARIAS, Marília Sa ...
2013 年 32 巻 2 号 p.
263-273
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
The purpose of this study was to evaluate the impact of replacing P90 primer with simplified adhesive systems (with a conventional, two-step adhesive or a self-etching, one-step adhesive) on bond strength to dentin in Class I restorations. The interfaces were also analyzed using confocal microscopy by adding a fluorophore to the adhesive components and SEM using silver nitrate for nanoleakage investigation. X-ray diffraction (XRD) characterized the chemical interaction of the adhesives. Failure mode and nanoleakage varied among groups. Characteristic micromorphology and higher nanoleakage were noted for silorane combinations. On the other hand, no signs of phase crystallization in the silorane adhesive combinations were noted. Replacing the silorane primer with simplified adhesives proved successful as the modified systems provided bonding to dentin comparable to that of the unmodified silorane systems. However, the dedicated adhesive exhibited signs of degradation immediately after application, which may impact the longevity of restorations in short periods.
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Toshihiko IIJIMA, Shinya HOMMA, Hideshi SEKINE, Hodaka SASAKI, Yasutom ...
2013 年 32 巻 2 号 p.
274-280
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
Hot isostatic pressing processed yttria-stabilized tetragonal zirconia polycrystal (HIP Y-TZP) has the potential for application to implants due to its high mechanical performance. The aim of this study was to investigate the influence of surface treatment of HIP Y-TZP on cyclic fatigue strength. HIP Y-TZP specimens were subjected to different surface treatments. Biaxial flexural strength was determined by both static and cyclic fatigue testing. In the cyclic fatigue test, the load was applied at a frequency of 10 Hz for 10
6 cycles in distilled water at 37°C. The surface morphology, roughness, and crystal phase of the surfaces were also evaluated. The cyclic fatigue strength (888 MPa) of HIP Y-TZP with sandblasting and acid-etching was more than twice that of Y-TZP as specified in ISO 13356 for surgical implants (320 MPa), indicating the clinical potential of this material.
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Seigo OKAWA, Kouichi WATANABE, Mitsugu KANATANI
2013 年 32 巻 2 号 p.
281-288
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
We report experimental results about the effect of polarity of electrode and anodized titanium oxide film on the deposited materials by electrolysis of an acidic calcium phosphate solution. Mirror-polished titanium and anodized titanium were used as anode or cathode, and a Pt plate was used as a counter electrode. The load voltage was held constant at 20 VDC. No deposited materials were found on the anode surface. On the other hand, dicalcium phosphate dihydrate (DCPD) was deposited on the cathode surface at the beginning of the electrolysis. After the electrolysis time 600 s, the non-stoichiometric hydroxyapatite (HAp) with several hundred nanometers was formed on the specimen surface. Based on X-ray photoelectron spectroscopy data, the anodized oxide film contained both P
5+ and P
3+ ions. This characteristic of the oxide film and the electrolysis conditions were related to the behavior of the deposition of ultra fine HAp with high crystallinity.
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Reina NEMOTO, Kosuke NOZAKI, Yuji FUKUI, Kimihiro YAMASHITA, Hiroyuki ...
2013 年 32 巻 2 号 p.
289-295
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
The purpose of this study was to evaluate the design of resin-bonded fixed partial dentures (RBFPDs) with zirconia frameworks. The abutment teeth were the upper central incisor and the canine. Three types of frameworks were fabricated as follows: 0.5-mm- and 0.8-mm-thick zirconia frameworks with grooves and holes (0.5ZrG, 0.8ZrG) and 0.5-mm-thick zirconia frameworks without grooves and holes (0.5Zr). The control group was designed as a 0.8-mm-thick metal framework with grooves and holes (0.8MG). Static loading was applied and the surface strain of the retainers was measured with strain gages. The magnitude of the principal strain of the 0.5ZrG framework was significantly lower than that of the 0.8MG and the 0.5Zr frameworks. This result suggests that the zirconia and retention form had a significant effect on decreasing the framework deformation, indicating that the RBFPDs that use a 0.5-mm thick zirconia framework are effective for replacing a single anterior missing tooth.
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Dimitrios DIONYSOPOULOS, Eugenia KOLINIOTOU-KOUMPIA, Maria HELVATZOGLO ...
2013 年 32 巻 2 号 p.
296-304
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
The aim of this study was to evaluate the fluoride release of five fluoride-releasing restorative materials and three dental adhesives, before and after NaF solution treatment. Five restorative materials (Fuji IX GP, GC Corp.; Ketac N100, 3M ESPE; Dyract Extra, Dentsply; Beautifil II, Shofu Inc.; Wave, SDI) and three dental adhesives (Stae, SDI; Fluorobond II - Shofu Inc.; Prime & Bond NT, Dentsply) were investigated before and after NaF solution treatment. A fluoride ion-selective electrode was to measure fluoride concentrations. During the 86-day period before NaF solution treatment, Fuji IX GP released the highest amount of fluoride among the restorative materials while Prime & Bond NT was the highest among the dental adhesives. After NaF solution treatment, Fuji IX GP again ranked the highest in fluoride release among the restorative materials while Fluorobond II ranked the highest among dental adhesives. It was concluded that the compositions and setting mechanisms of fluoride-containing dental materials influenced their fluoride release and recharge abilities.
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Maki KANETO, Yasuhiro NAMURA, Takahiko TAMURA, Noriyoshi SHIMIZU, Yusu ...
2013 年 32 巻 2 号 p.
305-310
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
The purpose of this study was to examine the use of electrolytic treatment, which can improve the corrosion resistance of Ni-Ti orthodontic wires, to minimize adverse effects. Electrolytic treatment of Ni-Ti wires was performed in a solution composed of glycerol and lactic acid for 5, 15, or 30 min. The anodic polarization test, three-point bending test, and X-ray photoelectron spectroscopic analysis of the wire surface were performed to explore an optimal treatment condition. Breakdown potentials of treated wires increased with increasing treatment time and higher corrosion resistance was obtained by performing the electrolytic treatment for more than 5 min. The relative concentration of nickel in the layer was decreased in inverse proportion to the treatment time. The results suggest that the commercial Ni-Ti wire with low corrosion resistance can be improved by the electrolytic treatment for more than 5 min.
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Baocheng CAO, Yuhua WANG, Na LI, Bin LIU, Yingjie ZHANG
2013 年 32 巻 2 号 p.
311-316
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
A bracket coated with a nitrogen-doped (N-doped) TiO
2-xN
y thin film was prepared using the RF magnetron sputtering method. The physicochemical properties of the thin film were measured using X-ray diffraction and energy-dispersive X-ray spectrometry, while the antimicrobial activity of the bracket against common oral pathogenic microbes was assessed on the basis of colony counts. The rate of antimicrobial activity of the bracket coated with nano-TiO
2-xN
y thin film against
Streptococcus mutans,
Lactobacillus acidophilus,
Actinomyces viscous, and
Candida albicans was 95.19%, 91.00%, 69.44%, and 98.86%, respectively. Scanning electron microscopy showed that fewer microbes adhered to the surface of this newly designed bracket than to the surface of the normal edgewise bracket. The brackets coated with the N-doped TiO
2-xN
y thin film showed high antimicrobial and bacterial adhesive properties against normal oral pathogenic bacterial through visible light, which is effective in prevention of enamel demineralization and gingivitis in orthodontic patients.
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Maretaningtias Dwi Ariani, Ayumu MATSUURA, Isao HIRATA, Takayasu KUBO, ...
2013 年 32 巻 2 号 p.
317-325
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
Carbonate apatite-chitosan scaffolds (CA-ChSs) were fabricated using the lyophilization technique. It was found that ChSs prepared with 200 mg chitosan powder (ChSs
200) had well-structured three-dimensional architecture with high porosity and good retentive form without brittleness. In addition, it was shown that the number of osteoblast-like cells MC3T3-E1 proliferated on desalinated ChSs
200 was larger than that on the non-desalinated ChSs
200. CA-ChSs were fabricated by adding 100 mg carbonate apatite (CA) to 200 mg chitosan gels followed by freeze-drying (CA
100ChSs
200). SEM observation revealed that CA
100ChSs
200 had favorable three- dimensional porous structures. The number of living cells increased more rapidly on CA
100ChSs
200 prepared with different amounts of CA than on ChSs. ALP activity significantly increased after day 14 and reached a plateau after day 21 in ChSs
200 and CA
100ChSs
200. It was concluded that newly developed CA
100ChSs
200 may be a possible scaffold material for tissue engineering.
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Abdul Aziz Abdullah Al KHERAIF, Sayed Saad Bin QASIM, Ravikumar RAMAKR ...
2013 年 32 巻 2 号 p.
326-331
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
This study sought to evaluate the effects of different staining solutions on the colour stability of nanocomposites compared with microhybrid resin and to evaluate the degree of conversion of these two materials. Two different shades of two different composites were cured in polytetraflouroethylene disk rings. Coffee, tea and cola drinks were used as staining solutions, and distilled water was used as a control. Data were statistically analysed using a paired T-test with a significance level of 5%. Nano composite showed the highest degree of conversion (DC) values based on calculation of the bonded and free carbonyl peak intensities in the spectrum. The colour analysis showed that nanohybrid had the highest Δ
E values when exposed to coffee solutions; they showed less color stability despite having a higher degree of conversion. Nano resin composite showed significantly higher discoloration than microhybrid composite.
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Sang-Hui YU, Dae-Hyung AHN, Ji-Su PARK, Yong Sik CHUNG, In-Sik HAN, Ju ...
2013 年 32 巻 2 号 p.
332-340
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
The aim of this study was to evaluate the effect of reinforcing polyaromatic polyamide (aramid) fibers with various orientations on the flexural properties of denture base resin. Aramid fibers with four orientations of unidirectional, woven, non-woven and paper-type were pre-impregnated and placed at the bottom of a specimen mold. Heat-polymerized denture base resin was packed over the fibers and polymerized. A three-point bending test was performed using a universal testing machine at a crosshead speed of 5 mm/min. The flexural strengths and flexural moduli of the unidirectional and woven groups were significantly higher than those of the control and other experimental groups.For the flexural moduli, all experimental groups showed significantly higher reinforcing effects than the control group. In conclusion, the unidirectional group located perpendicular to the direction of the load was most effective in reinforcing the denture base resin, followed by the woven group.
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Sadaaki MURAHARA, Hiroyuki MINAMI, Shiro SUZUKI, Kenji SAKOGUCHI, Dais ...
2013 年 32 巻 2 号 p.
341-348
発行日: 2013/03/28
公開日: 2013/03/29
ジャーナル
フリー
This study investigated the effect of adherend temperature on shear bond strengths of auto-polymerizing resin to denture base resin and 4-META/MMA-TBBO resin to silver-palladium-copper-gold (Ag-Pd-Cu-Au) alloy. Bonding procedure was carried out when adherend temperature was 10, 23, 37, or 55°C, and shear bond strengths (SBSs) were measured before and after thermocycling. Before thermocycling, there were no significant differences in bond strength among the four adherend temperatures for each adhesive resin: 31.59±6.11-32.89±2.12 MPa for auto-polymerizing resin; 35.43±2.2-38.38±0.61 MPa for 4-META/MMA-TBBO resin. After thermocycling, optimal adherend temperature to achieve the highest bond strength was 37°C for auto-polymerizing resin to denture base resin (30.02±2.29 MPa) and 10ºC for 4-META/MMA-TBBO resin to Ag-Pd-Cu-Au alloy (37.14±2.17 MPa).
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