This study used the adenosine triphosphate (ATP) swab test and the direct contact plate method to assess hospital kitchen hygiene and investigated inter-method consistency. Five specimens were collected on different days before and after cleaning for assessment with each method. The specimens were collected from 8 pre-defined sites in the kitchen of the Division of Nutrition, Shizuoka Cancer Center, between May 2016 and September 2016. The results of the methods were compared. Both methods showed significantly lower levels of contamination at all 8 sites after cleaning than before cleaning (p<0.001). Significantly fewer contaminations were detected by the ATP swab test (≥ 500 RLU) and direct contact plate method (≥ 11 cfu : 10cm²) after cleaning than before cleaning (p<0.01). An inter-method relationship was confirmed before cleaning (r=0.44, p=0.004). The ATP swab test and the direct contact plate method are both valid methods for assessing hospital kitchen hygiene. Pre-cleaning contaminations detected by the ATP swab test correlated with those using the direct contact plate method, suggesting the utility of the simple ATP swab test. Given the inter-method consistency, the two methods need to be used differently (ATP swab test : pre-cleaning ; direct contact plate method : post-cleaning). The methodology used in this study suggests that the ATP swab test method may make it possible to obtain true evaluations after washing/disinfecting of hospital kitchens.