From a clinical standpoint respecting the conditions of the extracted teeth, the writer devided the extraction cases into 3 groups, namely 1. (sound teeth) group, 2. (chronic focus) group and 3. (acute focus) group and has taken the bacteriological research of the tooth extraction wounds.
1) Experiment by aerobic culture from the extraction wounbs of 27 cases immediately after extraction.
In 2 cases of 1. group composed of 4 cases, the culture was negative. In the other 2 cases a few Sreptoc. viridans could be cultured in all cases but any difference in the species of bacteria between the cases with apical or marginal focus in both groups could not be found. In both groups Streptoc. viridans overwhelmed in number and the next was Staphylococcus. In the cases of 3. group the number of Staphyloc. hemolyticus (atypical) was larger and the rate of coming out of Bacllus coli group was higher than in that of 2. group.
The rate of coming out of bacteria in 2. and 3. group was summed up as follows
2. (chronic focus) group.
Staphylococci. 100%(Staphyloc. aureus 48.3%, of these strains 1 hemolyticus, Staphyloc. albus 91.6%), Streptococci 100%(1 case of these 12 cases Streptoc. homolyticus, Streptoc. viridans 100%, Streptoc. nonhemolyticus 48.3%), Bac. coli group 58.3 Microc. catarrhalis 16.6% Microc. tetragenus 8.3%, Bac. acidophylus 16.6% and obscure bacilli 48.3%.
2) The ebb and flow of the developmental condition of the bacteria in extraction wounds in the healing progress.
For the experimental object, Staphyloc. aureus, Stapyloc. albus, Streptoc. viridans and Bac. coli group were selected. In 1. group the number of bacteria of all species increased daily and showed the highest value in 2-3 days after the extraction. In 2. and 3. group it increased in 24 hours post extractionem rapidly and then increased or decreased in 3 days. In all cases the number of bacteria of each species was the smallest in 1. group and the largest in 3. group.
3) The test of the virulence and infectiousness for mice and rabbits.
(Injection: intraperitoneal in mice, subcutaneous in rabbits)
The animal were injected with the mixed cultured strains originated directly from the extraction wounds. The becteria stemmed from 2. group showed a little virulence for mice and slight infectiousness for rabbits. The bacteria stemmed from 3. group showed respectively more severe condition (weakness and death of mice, swelling, reddening and abscess in rabbits) for both animals than that of 2. group.
In the cases injected with single one of the vsrious species of bacteria isolated from 2. and 3. group, each strain, except some specific species, showed a little virulence and infectiousness for both animals. In the cases injected with the selective mixtures of two or three species isolated from 2. and 3. group, however, the results were more severe than in the cases injected with a single species. In this test the bacteria-mixtures of 3. group showed also more severe virulence and infectiousness than that of 2. group.
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