-
Hatsuhiko MAEDA, Naohiro ITO, Masayuki YOKOYAMA, Motohisa KAMIYA, Yoic ...
1995 Volume 44 Issue 3 Pages
317-321
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
The expression of epidermal growth factor receptor (EGFR) was investigated in the wound of hamster tongue. Fifty hamsters were used in this study. The tips of the tongues were excised with scissors, and then the animals were killed in groups of 5 at intervals of 1, 3, 5, 7, 9, 11, 13, 15 and 30 days. The tongues were studied histologically and immunohistochemically. Histologically, at 1, 3, and 5 days, the peripheral areas of wounds of all animals were covered with the parakeratinized epithelium, and the central areas of wounds exhibited fibrinous materials with some polymorphonuclear leukocytes on their surfaces. At 7, 9, 11, 13 and 15 days, the wounds were completely covered with the hyperkeratinized epithelium. At 30 day, the epithelia of the wounds were similar to the normal tongue epithelium. Immunohistochemically, at 1, 3, and 5 days, EGFR was uniformly present in keratinocytes at the migrating edge of the epithelium. At 7 day, the wounds were covered completely with the hyperkeratinized epithelium, and EGFR was present uniformly in all cell layers of this epithelium. At 9 and 11 days, however, EGFR was found to be reduced with time after 7 days. At 13 and 15 days, the basal cells only showed a positive staining for EGFR. At 30 day, the scattered basal cells expressed a positive staining for EGFR, the expression of which was similar to that of the normal tongue epithelium. It is suggested that the EGFR plays a regulatory role in the wound healing of the tongue.
View full abstract
-
Hiroyuki HAMAKAWA, Hiroaki TANIOKA
1995 Volume 44 Issue 3 Pages
322-327
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
An extraction method of cytokeratin (CK) from the human normal salivary gland is discussed and the CK distribution is analyzed by two-dimensional electrophoresis. Four normal submandibular glands showing no distinct microscopic change were prepared as the experimental material. Intermediate filaments (IF) were extracted in several solutions according to the modification of Achtstaetter. The CK was not sufficiently extracted in 1-2% SDS and 5% 2ME solution, however 11 bands of 58-40K M. W. were demonstrated in 9.5M Urea, 5% 2ME solution. Several fragments of the IF existed on the SDS polyacrylamide gel electrophoresis. It was considered that the fragmentation occured due to the existence of protease, as the variation of the homogenization with polytron had no influence on the fragmentation.
Immunoblotting reaction showed that the 11 bands reacted with two pan-cytokeratin monoclonal antibodies, e. g., K-8.13 that reacts with type II basic CK and C-11 that recognizes 4, 5, 6, 8, 10, 13, and 18.Using CK monospecific antibodies, #7, #8, #13, #18 and #19 reacted positively, but not #4 in any case. GFAP, neurofilament (NF) 68K, NF160K, NF200K also showed negative reactivity. Two-dimensional electrophoretic analysis confirmed the existence of the cytokeratin #5, #7, # 8, #13, #14, #15, #17, #18 and #19 in the hriman submandibular gland.
View full abstract
-
Immunoelectoron Microscopic Study
Junji HASHIMOTO
1995 Volume 44 Issue 3 Pages
328-343
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
Granular convoluted tubules in rat submandibular glands consist of granular cells (GC), pillar cells (PC) and transition cells (TC), and the granular cells show secretory granules which contain epidermal growth factor (EGF) and other biologically active peptides. The secretory granules of GC are released by alpha adrenergic stimulation. At the immunohistochemical level, PC and TC show an intense immunoreactivity for S-100 protein and calmodulin, the calcium binding proteins.
In the present study, immunohistochemical profiles of EGF and S-100 protein were evaluated in rat submandibular glands following administration of methoxamine (MX) to elucidate the secretory mecha-nisms in granular convoluted tubules and striated ducts (SD) using light and electron microscopy. In addition, S-100 protein in the submandibular gland and secreted saliva was evaluated by EIA.
Following administration of MX, the secretory granules of GC were released into the lumen of the ducts and the number of granules in GC was markedly decreased. Smooth surfaced blebs and separating zones were observed in the apical cytoplasm of GC and SD. Immunoelectron microscopy showed immunogold particles for S-100 protein in the blebs and cytoplasm of GC and SD, as well as those for EGF in the foamy, small granules and secretory pools of GC. Immunohistochemically, EGF was not detected in the GCT after 30 minutes, and EIA of the secreted saliva demonstrated S-100 proteins after 60 minutes of MX administration. The results of the present study suggest a possible role of S-100 protein in calcium signaling in the secretory mechanism of GCT and striated ducts.
View full abstract
-
Hitoshi NAKAMINE
1995 Volume 44 Issue 3 Pages
344-359
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
Bone morphogenetic protein (BMP) induces heterotopic osseous tissue via chondrogenesis in mouse skeletal muscle. Changes in extracellular matrix components during the chondro-osteogenetic process were evaluated by immunohistochemically using specific monoclonal antibodies for chondroitin, chondroitin 4 sulfate, chondroitin 6 sulfate, dermatan sulfate, keratan sulfate, and polyclonal antibodies for types I, II, and III collagens, fibronectin, and laminin. Labeling index of proliferating cell nuclear antigen (PCNA) was detected in the tissue structure.
Histochemically, the first stage of chondro-osteogenetic process was characterized by migration and proliferation of mesenchymal cells, the second stage coincided with development of chondroidal tissue formation, and the third stage was bone formation via endochondroidal ossification.
The chondro-osteoid matrix was mainly composed of glycosaminoglycans (GAGs), collagens, and other components of extracellular matrix. The distribution of GAGs, collagen, laminin, and fibronectin indicated a regulatory rule thourghout chondro-osteogenetic process. It is concluded that these macromolecules are synthesized and secreted by proliferating, transformed, or migrated cells in the process of chondro-osteogenesis, and that these components play an important role for heterotopic bone formation.
View full abstract
-
Reference to Cell Proliferation and Expression of p2lras and p53
Yoshiyuki OHNISHI
1995 Volume 44 Issue 3 Pages
360-375
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
In order to elucidate differences in biological behaviors between benign and malignant salivary gland tumors, the author examined cell proliferative activity by cytometric determination of DNA ploidy pattern and by PCNA and Ki-67 immunohistochemistry, and the expression of p 21 ras and p 53 protein was also examined by immunohistochemistry in 42 pleomorphic adenomas (PA) and 24 adenoid cystic carcinomas (ACC). When PAs compared with ACCs concerning DNA ploidy pattern, all PAs showed a diploid pattern without polyploid cells. Four cases of ACCs showed an aneuploid pattern, and other cases showed a diploid pattern with polyploid cells. The PCNA positive cell index (PCNA PI) of PAs and ACCs were 7.8 % and 16.3 %, respectively. The Ki-67 positive cell index (Ki-67 PI) of PAs and ACCs were 7.7 % and 16.8 %, respectively.
There was a correlation between PCNA PI and Ki-67 PI. The p 21 ras expression was observed in 54.8 % of PAs and in 66.7 % of ACCs. The p 53 protein was detected in 21.4 % of PAs and in 83.3 % of ACCs. Although there was no significant difference between PA and ACC for the expression of p 21 ras, a statistical significance in p 53 expression was found between PA and ACC.
View full abstract
-
Hideo KUROKAWA, Shoji TSURU, Hiroaki ISHIBASHI, Keiko MIURA, Akiko YAM ...
1995 Volume 44 Issue 3 Pages
376-383
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
Pleomorphic adenoma occurring in the oral cavity is neoplastic salivary gland tumor originating from the major and minor salivary glands and has the most frequent rate of occurrence among salivary gland tumors.
Although this tumor is said to be benign clinically, it has various structures, and recurrence and malignancy occasionally occur.
A histopathological and immunohistochemical investigation, therefore, was performed to elucidate these matters.
The results were as follows:
1. In histopathological structure of the 32 cases, chondroid pattern accounted for 18 cases (56.3 %), myxomatous pattern 15 cases (46.9 %), and cartilage pattern 10 cases (31.3 %). Squamous cell metaplasia was seen in 8 cases (25.0 %). Recurrence was seen in 3 (9.4 %) of the 32 cases and findings of myxomatous pattern were found in the 3 cases. Capsule was incomplete.
2. In histochemical investigation of this tumor, Periodic Acid Schiff (PAS) stain showed positive reactions at the portions of squamous cell metaplasia, myxomatous pattern, and cartilage pattern. Mucicarmin (MC) stain and Alucian-Blue (AB) stain showed positive reactions at the portion of myxomatous pattern.
3. In immunohistochemical investigation of this tumor, S-100 protein reacted negatively to normal myoepithelial cells and positively to tumorous myoepithelial cells and cartilaginous myoepithelial cells. Carcinoembryonic antigen (CEA) reacted positively to tumorous cells showing squamous cell metaplasia. Furthermore, localization of Keratin (KER) was suggestive of differentiation tendency toward squamous epithelium of tumorous myoepithelial cells.
On the basis of the foregoing investigation results, it was suggested that epithelial cells of the intercalated duct are the histogenesises of pleomorphic adenoma.
View full abstract
-
Junnosuke ISHII, Keikichi SHIMADA
1995 Volume 44 Issue 3 Pages
384-391
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
This study, using a carrageenan-induced rat paw edema, revealed that edema was inhibited by low power laser irradiation and demonstrated that two courses of irradiation produced a higher inhibition of edema than a single course.
Furthermore, the effect of low power laser treatment was evident on both the irradiated and nonirradiated sides.
As the effects extending to the opposite paw beyond the field of irradiation may have been mediated by the nervous system, particularly sensory nerves, experiments were subsequently performed using a rat model following sciatic neurotomy. In this model, the effect of low power laser treatment was evident on only the irradiated side.
In another model, using an adrenalectomized rat, it was not clearly observed on both sides.
Therefore, the results of this study suggest that the inhibitory action of low power laser treatment on carrageenan-induced edema is caused by some various effects through the sensory nerves and the adrenal glands.
View full abstract
-
Toshiyuki SHIBATA, Kazufumi WATANABE, Fumiyo MAKI, Motoyasu KATOH, Hir ...
1995 Volume 44 Issue 3 Pages
392-398
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
W e have previously reported that LEC (Long Evans Cinnamon) rat with spontaneous hepatitis and hepatoma is a very useful animal model for analysis of the pigmented teeth caused by bilirubin. In this study, to elucidate the mechanism of bilirubin pigmentation in LEC rat, we compared the LEC pigmented teeth with the experimental pigmented teeth caused by bile duct ligature and exogenous bilirubin administration to LEA rat, isolated from LE (Long Evance) closed colony accompanied with LEC rat, and we examined the chronological serum bilirubin and the pathological analysis of the pigmented teeth. By means of bile duct ligature of LEA rat, serum bilirubin level increased to 8.9 ± 1.1 mg/dl at the peak, but that of the pigmented teeth was not observed (0/9). As a result of this, intraperitoneal bilirubin was administered (14 mg/kg/day, for 4 days) in addition to bile duct ligature of LEA rat. Thus, serum bilirubin level increased to 13.7 ± 1.4 mg/dl at the peak and bilirubin pigmentation of the dentin was observed in 5 of 10 (5/10) rats, but there was no significant difference between the serum bilirubin level of the pigmented group and that of the non-pigmented group. Histopathological analysis of the LEA pigmented teeth using optical microscope showed that pigmentation of LEA rat was observed in incisors as a stripe in the dentin and the stripe ran parallel to the incremental line similar to that of LEC rat, and that the abnormal dentin tube was observed in the pigmentation area, but there was no correlation between abnormal dentin tube and teeth pigmentation. The microradiographic analysis disclosed enhanced permeabilities of the pigmented areas of all 5 rats, but the permeabilities of all 5 non-pigmented rats did not change. These results suggest that the hypocalcification of the matrix of the teeth is closely concerned with the bilirubin pigmentation in addition to the high level serum bilirubin.
View full abstract
-
Placement of Brånemark Implant to the Grafted Alveolus
Tetsu TAKAHASHI, Masayuki FUKUDA, Tai YAMAGUCHI, Shoko KOCHI, Keiko MA ...
1995 Volume 44 Issue 3 Pages
399-407
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
Alveolar cleft is the major problem during the formation of the ideal dental arch in cleft lip and/or palate patients. Since 1982, we have been treating patients with secondary alveolar bone grafting using autogenous particulate marrow and cancellous bone obtained from the iliac crest. Subsequently, teeth adjacent to the cleft into the grafted sites were repositioned orthodontically without any prosthesis. Sometimes, however, it is very difficult to achive the orthodontical closure of the arch without any prosthesis because of a too long orthodontical treatment period or a too wide cleft accompanied with congenitally missing teeth. Conventionally, prosthesis like denture or bridgework has been used for the dental reconstruction in these cases. Since 1993, we have applied Branemark Implant to the bone grafted alveolus in these cases. All patients with alveolar clefts received grafts of autogenous particulate cancellous bone obtained from the iliac crest except one case with periosteoplasty. After the bone bridge formation, orthodontical treatment and preparation for the implant placement were carried out. Seventeen Branemark fixtures were inlayed in 16 grafted alveoli. In 4 cases, in which the alveolar bone height was insufficient, chin bone grafts were used for the fixture placement. Free-standing final prosthesis was successfully applied in all cases.
This is the first report of a systemical application of osseointegrated implant in cleft lip and/or palate patients, and this treatment procedure is one of the optional methods for the dental rehabilitation of patients with alveolar clefts after secondary alveolar bone grafting.
View full abstract
-
Masashi YOSHIDA, Kazunori KAWANO, Kazumasa SUGIHARA, Sukehide YAMASHIT ...
1995 Volume 44 Issue 3 Pages
408-411
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
A new leucocyte depletion filter (Sepacell RN-20) used in Japan Red Cross Blood Center was evaluated to remove the cancer cells after filtration. Four kinds of cultured squamous cell carcinoma (HeLa S3, SQ 5, SCC TF and SCC KN) were examined with filters. No cancer cells existed in any filtered fluids without colony formations by clonometric assay. Cancer cells attached to polyester fibers of filters were observed by scanning electron microscopy.
These results suggested that cancer cells mingled in blood transfusion bags could be removed completely through a new leucocyte depletion filte
View full abstract
-
Comparison of Results in Salivary Glands and Peripheral Blood Mononuclear Cells
Chizuru ITO, Hiroyuki HAMAKAWA, Hiroaki KAYAHARA, Hiroaki TANIOKA
1995 Volume 44 Issue 3 Pages
412-421
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
According to recent studies, it seems that the host cells carrying cytomegalovirus (CMV) are not salivary gland cells but are lymphocytes and monocytes in the peripheral blood. However, this has not yet been clearly established because it is technically difficult to detect CMV-DNA in lymphocytes or monocytes from healthy CMV carriers by PCR methods.
In this study, the nested PCR method was employed for the detection of CMV-DNA in salivary 91ands obtained during surgery or autopsy and in peripheral blood mononuclear cells from healthy volunteers to figure out the target cells of the latent infection.
CMV-DNA was detected in 28%(14/50) of the salivary glands and in 85.2%(46/54) of the peripheral blood mononuclear cells. It is concluded that the target cells of CMV may be peripheral blood mononuclear cells.
The PCR methods by synthetic oligonucleotide primer pairs are so sensitive that it is useful to either protection against transfusion-transmitted CMV infection or rapid detection and grasp of CMV infection in immunocompromised patients induced by solid organ or bone marrow transplants. These are current clinical matters.
View full abstract
-
Toshikatsu MORI, Hideaki KAGAMI, Toshio SHIGETOMI, Minoru UEDA
1995 Volume 44 Issue 3 Pages
422-428
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
Burning mouth syndrome (BMS) is an intraoral pain disorder unaccompanied by clinical signs, including glossodynia and glossopyrosis. In this study we investigated clinical characteristics of BMS in forty-one patients using our protocol for oral discomfort. BMS in patients was mainly found in the climacteric period, especially in women. The most common accompanying diseases and medications were circulatory disorders and digestive, respectively. Not a few patients also complained of a dryness feeling with mouth and dysgusia, suggesting a complex background of this disease. Although laboratory testing revealed a tendency of high total cholesterol, high Cu
2+, and low Fe
2+, none of them were commonly detected in most of the patients. From psychological test or interview, patients with CMI high score (classIIf, IV) were 36.4% and cancer phobia or anxiety were observed in 51.8% of the patients. These results confirmed the important role of psychological factor in this disease.
View full abstract
-
Report of Clinical Course and Study on Neutrophil Function
Keiichi IGARASHI, Hiroto KIMURA, Wataru KOBAYASHI, Rou FUKUI, Tohru AK ...
1995 Volume 44 Issue 3 Pages
429-433
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
Pustulosis Palmaris et Plantaris (PPP) is a skin disease with relapsing and vesiculopustular eruption on the palms and soles. There are many reports that PPP is often caused by the focal infection, for which the therapy showed dramatic healing of these dermatosis. Recently, it was pointed out that phagocytic dysfunction of neutrophil was an important factor for the onset of PPP.
The authors experienced a case of chronic osteomyelitis of the mandible with PPP and report here its clinical course and superoxide (O
2-) production ability as a neutrophil function of the patient.
The patient was a 20-year-old female, whose chief complaint was diffuse swelling of mandibular region with PPP. The definitive diagnosis was easily obtained by the microscopic examination of the bone and Tc bone scintigram. After the mandibular osteomyelitis was surgicaly treated and continuously irrigated with antibiotics, the skin disease was remarkably relieved. Before the treatment, the O
2- production ability of the patient's neutrophil was lower than that of healthy controls. However, after surgical treatment, the O
2- production ability returned to the level of healthy controls.
These results and the clinical course strongly suggested that the osteomyelitis was a focal infection in PPP which might be related to neutrophil dysfunction.
View full abstract
-
Kazuhide NISHIHARA, Tamotsu MIMURA, Etsuro NOZOE, Kazuhiro MARUTANI, N ...
1995 Volume 44 Issue 3 Pages
434-437
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
A clinico-statistical analysis was carried out on the 372 primary patients with cleft lip and palate who hospitalized at our department during the 12-year period since 1981.
The results were as follows:
1. There were 137 cases (36.8 %) of cleft lip, 135 (36.3 %) of cleft lip and palate, and 100 (26.9 %) of cleft palate.
2. The total number of in-patients with cleft lip and palate was 697. It corresponded to 32.9 % of all in-patients at our clinic.
3. The distribution of 679 operations was lip repair 326 (48.0 %), palate repair 210 (30.9 %), lip revision 114 (16.8 %), closing oronasal fistula 7 (1.0 %), iliac bone graft, pharyngeal flap operation and rhinoplasty 3 (0.4 %) in each, and others 13 (1.9 %).
4. The peak incidence of lip repair was 4 months after birth, palate repair was 1 and half year of age, and lip revision was 1 year of age.
View full abstract
-
Fumihiko SHINOZAKI, Yoshikazu HAYATSU, Teruyo FUKUDA, Masayasu SUETSUG ...
1995 Volume 44 Issue 3 Pages
438-441
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
The hepatitis C virus (HCV) was identified by the Chiron group in 1989, using genetic engineering techniques, and it is presently believed to be the main etiologic agent for non-A non-B hepatitis.
Recently, we could detect antibody of hepatitis C virus (anti-HCV) using the test kits of RIA or EIA.
So, we investigated the prevalence of and-HCV in general practitioners among the Japanese dentists.
Of 382 practitioners, ten (2.6 %) were positive for anti-HCV. However, three of them had a history of transfusion. It is clearly well known that the most frequent infection is transfusion.
Therefore, these three were omitted and the positive rate of and-HCV was 1.8 %.
This result, ie, the difference between dentists and health care workers, which were reported by other investigators, was not significant. However, it is a little higher than that of blood donors data.
View full abstract
-
Minako YOSHIDA, Kazuhisa ESAKI, Shunichi TANAKA, Tamaka OKINA, Tadamit ...
1995 Volume 44 Issue 3 Pages
442-445
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
A case of a huge complex odontoma adhesion with the root of an impacted tooth in the mandible of a 35-year-old male is reported. The odontoma showed interesting developmental patterns. Some of the histological features are discussed.
View full abstract
-
Michio SHIKIMORI, Yoshio UEDA, Hiroshi FUKUDA, Kenji HASHIMOTO, Yumiko ...
1995 Volume 44 Issue 3 Pages
446-449
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
In the otolaryngological field, cancers involving the auditory cancer are relatively rare. Among them, the external auditory canal cancers (EACC) is found most frequently. Temporomandibular joint (TMJ) is resected simultaneously because of its positional closeness to the external auditorial canal. This paper outlines the treatment of TMJ in two cases of EACC, which were cured at the Department of Otolaryngology, Hamamatsu University Hospital.
Case 1 was a 63-year-old man with left EACC at the anterior wall, showing no clinical evidence of TMJ involvement. EACC was reduced in size after preoperative neoadjuvant chemotherapy. Its localization at the anterior wall was confirmed by CT. The tumor was surgically resected by piecemeal. No tumor invasion was clinically and pathologically detected at the posterial part of articular capsule. TMJ has been free from trouble one year and a half after surgery.
Case 2 was a 75-year-old male with right EACC at the anterior wall. Clenching produced pain in right TMJ. MRI revealed a mass in the external auditory canal. Piecemeal excision of the tumor was performed with resection of the posterior capsule wall and the disc. The defect of the wall was repaired with a temporal fascia flap. Disturbed mouth opening improved in a month.
Accurate evaluation of tumor infiltration prior to TMJ treatment may lead to the minimal effect on jaw movement. In the treatment of TMJ in EACC, preoperative evaluation, surgical plan, treatment of TMJ at operation, training of mouth opening and long-term follow-up are considered important.
View full abstract
-
Tadahide NOGUCHI, Yoko AKASAKA, Hitoshi OSANO
1995 Volume 44 Issue 3 Pages
450-454
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
W e experienced a case of secondary syphilis in a 31-year-old male with eruption of the oral mucosa. Defined grayish white surfaced erosion with raised margin was observed on the lower lip. Slightly raised grayish white lesion was observed on the apex and bilateral edges of tongue, and a well-defined grayish white lesion with redness on the palate was also observed. The serological tests for syphilis were positive, and
Treponema pallidum could be stained by Warthin-Starry method and immunofluorescene technique in the biopsy specimen taken from the lower lip and tongue. After the treatment with AMPC 1.5g/day for 8 weeks, and PCV 1.2 million unit/day for 4 weeks, the oral lesion disappeared in 3 weeks.
View full abstract
-
Hisao SHIGEMATSU, Shinya YAMAMOTO, Kazuya INOUE, Nobutoshi SHIONOYA, S ...
1995 Volume 44 Issue 3 Pages
455-458
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
Central odontogenic fibroma is a very rare lesion. A search of the Japanese language literature disclosed only 11 distinct cases. This article presents a case of cent ral odontogenic fibroma ocurring in the mandible and discusses the diagnosis.
A 74-year-old female was referred to our hospital on March 15.1994 with the chief complaint of an asymptomatic swelling of the right mandibular canine and premolar region that had persisted for about six months. Clinical examination revealed a hard swelling, measuring 21 X 21 mm. The overlying mucosa was normal in appearance and not tender to palpation. Radiographic examination showed well-circumscribed radiolucency with irregular zone of radioopacity between the first and second premolars. A tentative diagnosis of benign mandibular tumor was made. The lesion was resected easily and completely from the underlying bone. The enucleated neoplasm was a firm mass without a definite capsule. The postoperative course was uneventful ; and nine months after the operation, the patient had no evidence of recurrence.
Histopathological examination revealed a lesion composed of a densely cellular fibroblastic stroma. Througout the lesion many cords and strands of odontogenic epithelium were ob served. Based on these findings the lesion was considered to be central odontogenic fibroma (WHO type).
View full abstract
-
Katsuhiro HORIUCHI, Masamitsu HYOMOTO, Tadaaki KIRITA, Toyohiko KAMIBA ...
1995 Volume 44 Issue 3 Pages
459-466
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
In reconstruction of the mandible, the non-vascularized autogeneous iliac bone has been popularly used. Nowadays, the vascularized bones using microsurgery have many advantages over it.
During twelve years, twenty-three patients underwent mandibular reconstruction using bone grafts. Donor sites included the vascularized fibula (9), non-vascularized ilium (6), radial forearm flap with hemi-radius (5), scapular osteocutaneous flap (1), pedicled rib-latissimus dorsi osteocutaneous flap (1), and reimplanted bone after freezing and warming (1).
In 2 cases with the iliac bone graft, infection resulting from wound dehiscence occurred and was followed by great bone resorption. However, the grafted bone was not necessary to be removed.
All but one microvascular transfers have been completely successful. In order to overcome the main disadvantage of the fibular graft, low vertical height of the bone, a “double barrel” fibula was used in five cases, and patients acquired good results esthetically and functionally.
All patients with reconstructing marginal mandibulectomy, using the radial forearm flap, acquired good speech and oral function.
Vascularized bone grafts had advantages over the conventional iliac bone graft in the major mandibular defect, especially in the irradiated cases. It is concluded that the vascularized fibular graft, especially a “double-barrel” fibula, is superior to any other method because of its length, great flexibility in shaping the bone using multiple osteotomies, and minimal donor-site morbidity, and that it is the first choice for major mandibular reconstruction. On the other hand, the radial forearm flap is more suitable in reconstruction of the partial thickness of the mandible containing the oral floor.
View full abstract
-
Harumi MIZUKI, Yoshikuni FUKUYAMA, Masatsugu SHIMIZU
1995 Volume 44 Issue 3 Pages
467-471
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
-
Eiji HAYASHI, Koichi RIKIMARU, Kenji FUJISAWA, Fumihiro MATSUMOTO, Yas ...
1995 Volume 44 Issue 3 Pages
472-477
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
The diagnostic significance of
67Ga citrate and
99mTc MDP scintigraphy was evaluated in 47 patients with head and neck cancer.
The results were as follows:
1) In patients with squamous cell carcinoma the positive rate of primary lesion was 42.4 % in
67Ga citrate scintigraphy and 41.4 % in
99mTc-MDP scintigrapy
2) The positive rates in both
67Ga citrate and
99mTc-MDP scintigraphy were increased with the enlargement of tumor size and depended on the primary sites.
3) Computed tomography provided useful information in assessing primary lesion and metastases for the regional lymph nodes as compared with
67Ga citrate scintigraphy
4)
99mTc-MDP scintigraphy was useful to detect bone invasion, which could not be found by conventional radiographs.
5) In only one out of 47 cases, the distant metastases were suspected by both scintigraphies.
View full abstract
-
Nerve and Muscle Graft Related to Vascularized Free Latissimus Dorsi Musclocutaneous Flap, Serratus Anterior Muscle Flap, and Vascularized Free Dorsalis Pedis Flap
Yasuhiro SAKAMOTO, Yoshiyuki MORI, Yoshiyuki YONEHARA, Takahide KOMORI ...
1995 Volume 44 Issue 3 Pages
478-483
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
The development of microvascular surgery has made possible the closures of various complicated defects resulting from surgical removal of head and neck cancers. In these days, many kinds of vascularized free flaps can be transferred from various parts of a whole body to the oral and maxillofacial defects. We experienced two cases with recurrent adenoid cystic carcinoma of the right parotid gland and the right buccal mucosa respectively. Case 1 with the lesion of parotid gland was reconstructed with latissimus dorsi-serratus anterior muscle flap with its thoracodorsal nerve.
The development of microvascular surgery has made possible the closures of various complicated defects resulting from surgical removal of head and neck cancers. In these days, many kinds of vascularized free flaps can be transferred from various parts of a whole body to the oral and maxillofacial defects. We experienced two cases with recurrent adenoid cystic carcinoma of the right parotid gland and the right buccal mucosa respectively. Case 1 with the lesion of parotid gland was reconstructed with latissimus dorsi-serratus anterior muscle flap with its thoracodorsal nerve.
Case 2 with the lesion of right buccal mucosa was reconstructed with free vascularized dorsalis pedis flap. Many authors have reported about application of forearm free skin flap to the reconstruction of oral cavity. However, very few reports of dorsalis pedis free skin flap in those areas had been presented. This thin flap seems to be suitable for the reconstruction of the oral cavity. And the dorsalis pedis free skin flap has also the cosmetic advantage of the donor site.
These functional and cosmetic restorations could facilitate the return to the social life of the patients.
View full abstract
-
Masashi TSUZUKI, Tetsuji KAWAKAMI, Ken-ichi TAKAYAMA, Norimasa OHKOCHI ...
1995 Volume 44 Issue 3 Pages
484-490
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
Clinico-statistical observation and radiological analysis of temporomandibular joint luxation were reported.
One hundred forty-three patients visited our clinic from October 1981 to September 1993. The patients were 51 males and 92 females, with an average age of 46.3 years, ranging from 14 to 92 years ; the sex ratio was 1: 1.9 ; and there were 74 bilateral cases and 69 unilateral cases.
The patients were divided into 3 groups, acute-simple (51 cases), long-standing luxation (8 cases) and habitual luxation (84 cases). The most common location of onset was the jaws (113 cases), and the most common was direct visit (69 cases). Seven patients, who visited our clinic within 6 months after luxation, had long-standing luxation, 35 patients visited our clinic within 6 hours after luxation, and 54 patients had havitual luxation.
The authors performed radiological analysis, to measure height of articular tubercle, depth of mandibular fossa, angle of anterior surface in articular tubercle, and course of condyle, on 85 cases.
View full abstract
-
Relationship between the Findings of Angiography and the Effect of Embolization
Takeshi MATSUSHITA, Kouichirou OKAMOTO, Yasushi OHASHI, Tatsuhiro SEI, ...
1995 Volume 44 Issue 3 Pages
491-495
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
Two cases of giant hemangioma of oral region treated by transcatheter embolization are reported in this article.
Case one was a 2I-year-old female visited our hospital complaining of swelling of the tongue and oral floor. External carotid angiography showed a vascular lesion mostly fed by the bilateral facial arteries, and showed clear tumor stain. Transcatheter embolization using Ivalon® was carried out with digital subtraction angiography. After embolization the tumor stain and the distal parts of main feeders were not visualized on angiography, and then the tumor size was decreased clearly.
Case two was a 25-year-old man, who came to our hospital complaining of speech disturbance due to swelling of the tongue. ECAG showed a vascular lesion fed by the left lingual artery, but tumor stain was not visualized. We tried transcatheter embolization without excising the tumor, taking into consideration of the functional aspects. After embolization, the distal part of the main feeder was not demonstrated. After treatment, a considerable decrease in the size of the lesion was observed.
From these experiences, it was considered that embolization was one of treatment methods of the hemangioma of oral and maxillofacial regions, and that angiography gave many informations about its indication and effect.
View full abstract
-
Kaori YAGO, Youichi TANAKA, Hiroshi IWABUCHI, Hiromasa HONMA, Yutaka O ...
1995 Volume 44 Issue 3 Pages
496-505
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
One hundred and seventy-three epithelial cysts of the jaws from 171 patients were analized in the Department of Dentistry and Oral Surgery, School of Medicine, Keio University.
The following results were obtained.
(1) Epithelial lining of the cyst wall was not seen in 16 of 84 radicular cysts (19%) because of inflammation. These were diagnosed as radicular cysts by clinical features.
(2) Dentigerous (follicular) cysts (48 cysts) were classified into 3 groups.
Group 1: Typical dentigerous cysts (20 cysts).
The epithelial lining consisted of 2-3 cell layers of flat or cuboidal cells, and resembled reduced enamel epithelium.
Group 2: Inflamed dentigerous cysts (14 cysts).
Group 3: Intense inflamed dentigerous cysts (14 cysts).
Histologically these had the epithelial lining which resembled that of radicular cyst
(3) About half of the cases of odontogenic keratocysts were found to have a relationship with an impact.ed tooth. Keratinization was predominantly parakeratotic, but 5 of all odontogenic keratocysts (17.2%) were orthokeratotic.
(4) One case of primordial cyst which had non-keratinized epithelial lining was found. It did not fulfill the criteria for odontogenic keratocysts as stated by Pindborg.
(5) In regard to 11 cysts which were diagnosed as so called fissural cysts, the epithelial-lined cysts of non-odontogenic origin were thought to be derived from embryonic epithelial residues. It was difficult to distinguish them from that of odontogenic origin except nasopalatin duct (incisive canal) cyst and nasoalveolar cyst.
It is considered important that oral surgeon and pathologist exchange clinical and pathological information in detail with each other for diagnosis of the cyst in the jaws.
View full abstract
-
Hideaki KAGAMI, Iwai TOHNAI, Minoru UEDA
1995 Volume 44 Issue 3 Pages
506-508
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
This paper presents a case of huge pleomorphic adenoma of the parapharyngeal space. The patient was an eighteen-year-old male with right side buccal swelling. and pain was first noted six months before the first examination. The MRI and CT study indicated a tumor-like region extending widely at the site of parapharyngeal space and situated beneath the cranial floor between internal and external pterygoid muscles. The tumor-like mass was demarcated clearly as ovoid in shape. The e was no indication of bone resorprion. The region was thus considered as a bening tumor, such as pleomorphic adenoma or neurilenmoma. It was extirpated extra-orally under general anesthesia. The tumor was encapsulated in fibrous tissue. Its dimensions were 65×50×40mm in size and weight 75g. The connection with major salivary gland could not be found. The pathological diagnosis was typical pleomorphic adenoma which providing a mixomatous pattern. In the tissues investigated, no apparent invasion could be found in surrounding capsule.
View full abstract
-
The Quantitative Analysis of Cognition for the Bony Maxillofacial Asymmetry
Satoshi YOKOO, Osamu TERANOBU, Arata IGUCHI, Takashi SHIMAZAKI, Takahi ...
1995 Volume 44 Issue 3 Pages
509-517
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
The quantitative diagnosis of maxillofacial deformity is hampered by the fact that people have an asymmetrical face, even though it appears to be normal and symmetrical. Only a few attempts have so far been made to develop specific quantitative diagnostic criteria for maxillofacial asymmetry. The purpose of this study was to establish some quantitative diagnostic criteria by which maxillofacial asymmetry diagnosable by cursory inspection can be differentiated from asymmetry within the normal range, and the following results were obtained.
1) A specific statistical chart of asymmetrical indexes showed by posterioanterior cephalogram constructed based on values obtained in Japanese with maxillofacial features classified as symmetry upon observation.
2) The values of asymmetrical indexes for Go, GAr and Me in patients with mandibular prognathism were all within the normal range.
3) The values of asymmetrycal indexes for Go, GAr and Me in patients with asymmetrical prognathism were all beyond 2SD. However, when asymmetrical index values for Mo were between 1SD and 2SD, deviation of only the mandible was diagnosed of which the patients had made only this complaint.
4) The asymmetrical index values for CMo and Mo in patients whose complaint was distortion of the maxilla were beyond 2SD.
5) By means of these facts, we established a specific quantitative method of classification of maxillofacial asymmetry in which maxillofacial asymmetry was defined as symmetrical index value for each main point (CMo, Mo, Go, GAr, Me) of more than 2SD.
6) It is suggested that the deviation beyond 2SD from the average may be a diagnostic criterion in quantitative analysis of maxillofacial asymmetry.
View full abstract
-
Mikiko MITSUFUJI, Tadamitsu KAMEYAMA, Minako YOSHIDA, Osamu IWAMOTO, K ...
1995 Volume 44 Issue 3 Pages
518-526
Published: July 10, 1995
Released on J-STAGE: September 07, 2011
JOURNAL
FREE ACCESS
Case analysis was performed or 1182 cases who were admitted to the ward of the department of oral surgery, Kurume University Hospital, Kurume Japan, for 5 years-during period from January 1st, 1989 until December 31st, 1993. In this report, the results of present analysis were clinicalstatistically compared with previous report of our ward, in which 706 cases were analized for 3 years and 10 months-during period from July 1, 1973 to April 30, 1977.
The results were as follows:
1. As for age distribution, infants and children less than 9 years old were greatly reduced in proportion (present/previous analysis: 40%/4.6%). on the other hand, the aged were increased. Especially in in patients over 65 years old, the difference between two periods (19.6%/6.9%) was significant (P<0.001).
2. As for disease, malformations were remarkably reduced, cancers increased. Almost half of the patients over 65 years old had cancer. In fracture cases of maxillofacial region, fresh cases of only jaw were increased and cases with severe complications were reduced.
3. Inpatients from southern areas of Fukuoka Prefecture were increased (71.2%/48.4%). This result indicated that a sphere of regional distribution of patients became narrow.
4. There was a significant increase in the ratio of patients with general disease (23.8%/4.8%).
View full abstract
