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Volume 18 (1985)
- Issue 6 Pages 565-
- Issue 5 Pages 467-
- Issue 4 Pages 365-
- Issue 3 Pages 275-
- Issue 2 Pages 139-
- Issue 1 Pages 1-
Volume 18, Issue 4
Displaying 1-11 of 11 articles from this issue
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MARIA LUIZA S. MELLO, BENEDICTO DE C. VIDAL1985 Volume 18 Issue 4 Pages 365-373
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe autofluorescence of the Malpighian tubules of blood-sucking insects was studied microspectrofluorimetrically. Spectral emission profiles were determined for the naturally fluorescent substances present at the lumen of the proximal region and in the cytoplasm of the distal region of the organ. These were compared with the emission profiles exhibited by standards of fluorescent substances generally found in insect Malpighian tubules. The fluorescence detected at the proximal region was then concluded to be provided by uric acid whereas that of the distal region was attributed to kynurenine. The results did not vary as a function of the developmental stage of the insects. If pteridins are present in the tubules, they appear not to affect the fluorescence patterns determined in this work.View full abstractDownload PDF (2757K) -
KEIICHI MORIGUCHI, KEI-ICHI HIRAI1985 Volume 18 Issue 4 Pages 375-381
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSN-formyl-methionyl-leucyl-phenylalanine (FMLP)-stimulated hydrogen peroxide (H2O2) production in peritoneal macrophages and the effects of anti-inflammatory drugs and local anesthetics on it were electron cytochemically demonstrated using the cerium method. Resident peritoneal macrophages were harvested from adult male guinea pigs and treated with 5μg/ml cytochalasin B for 15min followed by an FMLP (1μM) treatment for 10min. The processed cells were incubated in a cerous medium in order to precipitate H2O2 moieties produced by the cells. FMLP induced cell aggregation, and the H2O2 production was seen on the plasma membrane sites where the cells had contacted. The reaction was markedly inhibited by p-benzoquinone and ethylenediamine-tetraacetate. Aspirin, indomethacin and lidocaine, but not dexamethasone, significantly reduced cell aggregation resulting in a decrease in the number of H2O2 productive cells. These results indicate that FMLP-stimulated H2O2 production in macrophages is initiated by calcium ions and inhibited by some anti-inflammatory drugs and local anesthetics in accord with reported physiological results, and that cell-cell contact is essential to H2O2 production.View full abstractDownload PDF (10125K) -
AVIDIN-BIOTIN-CHOLERAGEN AND AVIDIN-BIOTIN ANTIBODY COMPLEX STAINING METHODSHIROAKI ASOU, NORIKO IWASAKI-MUTOU, SHUSUKE HIRANO, TAKASHI TURUMIZU, ...1985 Volume 18 Issue 4 Pages 383-389
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA new technique demonstrating the presence of ganglioside GM1 in neurons, neurite bundles and oligodendrocytes using avidin-biotin complex systems in double staining techniques is reported. The method was applied to cultured brain cells.
The ganglioside GM1 may be present in the growth cones of outgrowing neurites in these living cell cultures.View full abstractDownload PDF (6625K) -
MITSUO MACHINO, HIROYUKI MORIOKA, MASAYOSHI TACHIBANA1985 Volume 18 Issue 4 Pages 391-394
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSUltrathin sections of the serous acinar cells in human parotid glands stained with a complex of wheat germ agglutinin and gold were examined with an electron microscope. Gold particle labeling was mainly detected in the secretory granules, condensing vacuoles, and cisternae of the maturing face of the Golgi complex. The peripheral rim of the serous granule matrix was heavily stained, and the condensing vacuoles and vesicular cisternae at the maturing face of the Golgi complex were moderately labeled. The central dense cores in the serous secretory granule were labeled sporadically with gold particles. The distributional pattern observed here was very similar to that observed in various kinds of mucous cells. The significance of the distribution pattern of reaction products is briefly discussed.View full abstractDownload PDF (2872K) -
PARVINE NOUHNEJADE, SHOHREH MALEKI1985 Volume 18 Issue 4 Pages 395-401
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSDistribution and localization of dantrolene-Na in the organs of mice has been studied, using whole body frozen sections by the aid of fluorescent macro-and microscopic techniques. The results obtained in these experiments were compared to the tissues prepared by wax-embedding techniques. Uptake of the drug by isolated rat organs and dissected fresh locust thoracic muscle has also been examined.
Based on present studies, dantrolene-Na was not distributed uniformly throughout the body. It was mainly accumulated in intestine and liver and partly in kidneys. Dantrolene accumulation was not dose-dependent. However, length of administration time was quite effective on the pattern of distribution. At shorter intervals it was mainly accumulated in intestine, liver and slightly in kidney. Longer exposure to the drug resulted in more intense accumulation in intestine and liver.
Comparison of frozen and fresh material to fixed sections further supports the previous investigations that fixation procedures grossly alter the normal state of tissues and inevitably give rise to artifactual observations.View full abstractDownload PDF (7459K) -
KOUTA YAMAMOTO, NOBUKAZU ARAKI, KAZUO OGAWA1985 Volume 18 Issue 4 Pages 403-418
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe effect of preadministration of Xiao-Chai-Hu-Tang on hepatic disorders, induced by D-galactosamine HCl, were examined morphologically and histocytochemically. A single intraperitoneal injection of 800mg/kg or 1500mg/kg D-galactosamine HCl induced remarkable histo- and cytologic changes in the rat liver. Light microscopically, the liver showed diffuse parenchymal damage, in which hepatic cell cords were disorganized and a marked accumulation of lipid droplets were found in the hepatocytes. Ultrastructurally, disruption of lamellar arrangement of rough endoplasmic reticulum, dissociation of intrahepatic cell space and an increase in the number of autophagic vacuoles were observed in the control groups after the administration of D-galactosamine HCl. Histo- and cytochemical detection of 5′-nucleotidase and alkaline phosphatase activities revealed disruption of the bile canaliculi system and a disturbance of plasma membrane. Activity of the glucose-6-phosphatase disclosed an uneven distribution of enzymes cell by cell.
However, no conspicuous pathological and histocytochemical changes were found in the liver preadministered with Xiao-Chai-Hu-Tang. Biochemical assay revealed that much higher enzyme activities were preserved in the groups preadministered with Xiao-Chai-Hu-Tang. From these results, it can be seen that the preadministration of Xiao-Chai-Hu-Tang is undoubtedly effective in preventing changes induced by D-galactosamine HCl.View full abstractDownload PDF (19159K) -
RYOICHI SHIMONO1985 Volume 18 Issue 4 Pages 419-431
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSTissue and organ distribution and metabolic fate of radioactive carbon from [U-14C] galactose in mice were studied by whole-body autoradiography and biochemical analysis.
Autoradiography was performed before and after 6% perchloric acid treatment at various intervals after intravenous injection of [U-14C] galactose. At 5min after injection, high radioactivities were observed in the kidney, small intestine and liver. The radioactivities in almost all organs were markedly decreased with the acid treatment. At 30min after injection, the kidney and small intestine had relatively high radioactivities which were retained by the acid treatment.
As for the biochemical analysis, at various intervals after intravenous injection of [U-14C] galactose, the mice were decapitated, and the blood, liver, kidney, small and large intestines, myocardium and brain were removed. These organs were fractionized into 6% perchloric acid-soluble, -insoluble and lipid parts. Total radioactivity (cpm) per gram of wet weight was the highest in the kidney. The incorporation rates of the radioactivity into the acid-soluble fraction were high in all organs at early intervals, while at late intervals, the rates into the acid-insoluble fraction became relatively high in the kidney, small and large intestines. These results were consistent with those obtained from whole-body autoradiography.
Acid-soluble fractions of the blood, kidney, liver and brain were analyzed by paper chromatography. The radioactive spots for galactose and glucose were observed in all organs examined, but the changes of radioactive values with the passage of time were different in each organ. Furthermore, the radioactive spots for RGlucose value 0.28 were detected in these organs.View full abstractDownload PDF (3586K) -
HIROFUMI TERUBAYASHI, HAJIME FUJISAWA, MOTOKAZU ITOI, YASUHIKO IBATA1985 Volume 18 Issue 4 Pages 433-438
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSWe examined retinal projections to the hypothalamus in albino rats at early postnatal stages by means of injecting horseradish peroxidase (HRP) into the eye and observing the anterograde transport. On the 1st postnatal day, HRP-filled retinal fibers reached the suprachiasmatic nucleus (SCN) on the bilateral sides to the eye injection of HRP, and made synaptic connections with dendrites of the SCN neurons. The retinal projection to the lateral hypothalamic area (LHA) on the contralateral side to the eye injection of HRP was also noted at the 1st and 3rd postnatal days, but not on the 7th and 10th postnatal days or in the adults.View full abstractDownload PDF (7502K) -
MOSHE WOLMAN, DAN FUNDOIANU-DAYAN, MOSHE PERLMUTTER1985 Volume 18 Issue 4 Pages 439-443
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSPieces of Achilles tendons obtained from 15 autopsies and 17 amputated legs were cut at a standard thickness and examined for blue autofluorescence in relation to the patients' ages. The intensity of fluorescence was estimated subjectively as well as densitometrically with a photomultiplier. Fluorescence increased in intensity after the fifth decade in tendons obtained at autopsy. No age dependence was noted in tendons of amputated legs.
It is suggested that the blue fluorescence is due to pyridinoline or similar crosslinks. The fluorescence intensity can serve as an indication of age. The effect of ischemia requires further study.View full abstractDownload PDF (1388K) -
KOICHI IIJIMA, NAOSUKE KOJIMA1985 Volume 18 Issue 4 Pages 445-454
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe pharmaco-histochemical method with gabaculine treatment for selectively detecting the enzyme 4-aminobutyrate: 2-oxoglutarate (EC. 2.6.1.19.: GABA-T) in neuronal perikarya was applied to the rat supraoptic nucleus (SO) to detect GABA-T-intensive, presumably GABAergic neurons in and around the SO, and if possible, to examine their relationship to neurosecretory neurons.
A significant number of medium-sized GABA-T-intensive neurons were detected in the dorsal part, while more numerous, small, moderately GABA-T-positive neurons were detected in the ventral part of the perinuclear zone just dorsal to the SO. Inside the SO, no GABA-T-intensive intrinsic neurons were detected, although a few small, mildly GABA-T-positive ones were detected in a few cases. No projections of these GABA-T-intensiven eurons to the SO were detected. The unreactive cell bodies of some neurosecretory neurons located in the dorsal margin of the SO as well as in discrete groups scattered throughout the SO were surrounded by granular reaction products for GABA-T.
The results strongly suggest that the medium-sized GABA-T-intensive neurons in the perinuclear zone send their axons to the SO, where they richly divide into many branches which surround the cell bodies of some oxytocin and vasopressin cells with abundant GABA-T-positivea xon terminals.View full abstractDownload PDF (6405K) -
A STUDY BY LABELING FROZEN THIN-SECTION WITH CATIONIZED FERRITINKAZUSHI FUJIMOTO, KAZU SHIGE OGAWA, SETSUKO KONDO, KAZUO OGAWA1985 Volume 18 Issue 4 Pages 455-463
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe distribution of anionic sites on intracellular organelles were studied by labeling frozen thin-sections with the multivalent ligand, cationized ferritin, as a visual probe.
Frozen thin-sections obtained from fixed rat cardiac muscles with 0.5% glutaraldehyde were incubated with cationized ferritin (50μg/l ml phosphate buffered saline, pH7.4) for 30min at room temperature.
The ligands almost continuously labeled the surface of the sarcolemma and the junctional membrane forming the intermediate junction and desmosome, and the luminal surface of the T-tubules. In mitochondria, the ligand binding occurred to the outer surface of the outer mitochondrial membranes and to the matrix side of the inner mitochondrial membranes. High affinity for the ligand was seen on the cytoplasmic side of the gap junction. In addition, the sarcoplasmic reticulum and thick filaments showed moderately high affinity for cationized ferritin. The implications of our observations in the regulation or maintenance of cellular organization are discussed.View full abstractDownload PDF (15032K)
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