All issues
Volume 21 (1988)
- Issue 6 Pages 521-
- Issue 5 Pages 439-
- Issue 4 Pages 321-
- Issue 3 Pages 215-
- Issue 2 Pages 91-
- Issue 1 Pages 1-
Volume 21, Issue 2
Displaying 1-11 of 11 articles from this issue
- |<
- <
- 1
- >
- >|
-
CHIHARU TOHYAMA, HISAO NISHIMURA, NORIKO NISHIMURA1988 Volume 21 Issue 2 Pages 91-102
Published: 1988
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe localization of metallothionein (MT), a small molecular weight heavy-metal binding protein, in the liver and kidney of rats treated with either cadmium (Cd) chloride or zinc (Zn) sulfate was investigated by the indirect immunofluorescent technique. Male Wistar rats were subcutaneously injected with CdCl2 at a single dose of 3.0 mg Cd/kg body weight or 50 mg Zn/kg body weight, followed by sacrifice at 3, 6, 12 and 24 hr. For comparison, tissues were also obtained from rats treated with 1.5 mg Cd/kg body weight, 4 times a week for 6 weeks. In control rats from both short- and long-term studies the MT antigenicity was detected in the cytoplasm of hepatocytes and proximal tubular cells. A major difference in the localization of MT between the two studies was found only in the liver. The immunoreactive stain was limited to the hepatocytes around both central and interlobular veins in the long-term study compared to rather uniform localization throughout the liver in the short-term study. The intensity of immunostain of MT in both organs upon Cd or Zn treatment tended to increase with tissue concent-rations of the protein, as determined by a radioimmunoassay. Cd and Zn treatment resulted in the localization of MT in a similar manner. In particular, the number of proximal tubules with immunofluorescence increased upon an injection of Cd or Zn, and once a renal Cd level considerably increased by the repeated injections of Cd, intense stain of MT was observed not only in the proximal tubule but also the distal tubule and collecting duct. No specific immunostain was observed in the glomerulus, vascular endothelial cells and connective tissues. Although not only the cytoplasm but also the nucleus of the hepatocytes and proximal tubular cells often showed immunofluorescence, no significant change in the induction of MT between these subcellular compartments with time was found.View full abstractDownload PDF (4298K) -
ITSURO HIGUCHI, MASANORI NAKAGAWA, MITSUHIRO OSAME, TAKASHI YAMANO, HI ...1988 Volume 21 Issue 2 Pages 103-111
Published: 1988
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSImmunohistochemical examinations using monoclonal antibody against subunit IV of cytochrome c oxidase were carried out in the muscle of patients with various mitochondrial myopathies having histochemical partial deficiency of cytochrome c oxidase activity. The ragged red fibers showed positive reaction for the monoclonal antibody even in the histochemical cytochrome c oxidase deficient fibers. However, in the histochemical cytochrome c oxidase deficient fibers with normal histological appearance, the immunohistochemical reaction varied. While all fibers with immunohistochemical deficient reactivity for subunit IV of cytochrome c oxidase had no detectable histochemical cytochrome c oxidase activity, subunit IV protein deficiency was not the cause of all the histochemical cytochrome c oxidase deficient fibers.View full abstractDownload PDF (2183K) -
TADASHI OKUTOMI, NORICHIKA TATEMATU, NOBUMITSU OKA, SHUNSUKE KUMASA, M ...1988 Volume 21 Issue 2 Pages 113-124
Published: 1988
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSTwo cases of congenital epulis were examined immunohistochemically for the presence of keratins, desmin, vimentin, involucrin, S-100α and β proteins, lysozyme, lactoferrin, α1-antitrypsin, and α1-antichymotrypsin, as well as for lectin binding patterns. For the latter, 7 kinds of lcctins (ConA, RCA-1, DBA, SBA, PNA, WGA, and UEA-1) were employed. Granular cells were negative for all of the immunohistochemical reactions carried out. However, these cells did give slightly positive staining with RCA-1, PNA, and WGA lectins, suggesting galactose (Gal), N-acetyl-D-galactosamine (GalNAC), N-acetyl-D-glucosamine (GluNCA) residues in granular cells.
A comparison in immunohistochemical evidence was made between granular cells in congenital epulis and those in other granular cell tumors. Different properties for immunohistochemical reactions were given between the granular cells of congenital epulis and those of other granular cell tumors. We propose the term “congenital granular cell tumor”instead of “congenital epulis”, in order to discriminate this type from other granular cell tumors. The histogenesis of granular cells in congenital granular cell tumor was different to neutral origin.View full abstractDownload PDF (3224K) -
HISASHI HASHIMOTO, KAZUMASA HOSHINO1988 Volume 21 Issue 2 Pages 125-136
Published: 1988
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSImmunohistochemical localization of laminin was investigated in fetal lungs of mouse. The antigenicity of laminin was lost during the process of fixation and paraffin embedding, but restored well by the 60 min pepsin treatment.
Laminin was already detected in Day 12 fetal lungs and clearly demarcated the tubule of the lung primordium. In Days 14 and 16 fetal lungs, laminin was fouad at the epitheliomesenchymal boundary of any portion of the tubule, and the proximal portion showed stronger immunoreaction than the distal portion. In Days 17 and 18 fetal lungs, the immunoreaction became generally weak, but the reaction products clearly demarcated the epitheliomesenchymal boundary.
In both Day 14 and Day 16 fetal lungs, the effects of the pepsin treatment at different time intervals were explored. Without the pepsin treatment, no peroxidase reaction products were found on any part of the tissue section. When treated with pepsin for 15 min, the immunoreaction became positive at the distal portion of the tubule. After the pepsin treatment for 30 min, the immunoreaction became intense at the distal portion and appeared at the proximal portion of the tubule. The pepsin treatment for 60 min generally intensified the immunoreaction of laminin. However, after the pepsin treatment for 120 min, the immunoreactivity became rather weak at the distal portion of the tubule, but was unaltered or became stronger at the proximal portion and at the bronchus.
These results indicate that the rapidly growing or expanding areas in the distal portion of the tubule of fetal lungs appear to be sustained often weakly by the basement membrane which is considered to be immature in its compositions, constituents or matrix assembly, while immunoreactive products of laminin in the basement membrane along the boundary between the undifferentiated epithelium and the adjacent mesenchyme appears consistently in a thick belt-shape.View full abstractDownload PDF (3694K) -
SHASHI WADHWA, VEENA BIJLANI1988 Volume 21 Issue 2 Pages 137-142
Published: 1988
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDevelopment of substance P-containing nerve fibers was studied in eight human fetal urinary bladders obtained from fetuses of 12 to 26 weeks gestation using monoclonal antibodies against substance P. At 12 weeks occasional fibers were seen in the trigonal submucosa and bladder wall while fine fiber plexuses, in fewer amount, were present along the blood vessels entering the bladder. Increasing proportion of thick substance P fibers were seen in 15, 19 and 26 week trigonal submucosal blood vessels and muscle layers. Occasional immunopositive fibers were also observed to enter the epithelium.View full abstractDownload PDF (1724K) -
CHISATO MORI, HISASHI HASHIMOTO, SENKITI SAKAI, KAZUMASA HOSHINO, TAKU ...1988 Volume 21 Issue 2 Pages 143-149
Published: 1988
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (1910K) -
TAKUZO ODA, SHOGO IKEDA, YUSEI ARAKAKI, TSUNENORI MATSUBARA, MASASHI M ...1988 Volume 21 Issue 2 Pages 151-163
Published: 1988
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSMolecular cytochemistry of nucleic acids by gene technology and the advances in its application are reviewed with special reference to in situ hybridization. Studies on the in situ hybridization of repetitive and unique DNA sequences, messenger RNA, and viral genomes are outlined. A brief survey of the methods employed for labeling DNA and RNA probes with high specific radioactivity or nonradioactive markers and sensitive detection systems are given. The data of our own studies on the detection of Y chromosome by in situ hybridization with biotin-labeled DNA probes, molecular cloning of proviral DNA of a retrovirus produced in a human lymphoblastoid cell line, and in situ detection of gene expression of the molecularly cloned proviral DNA in transfected cells are also presented. The gene expression was detected in several percent of the transfected cells by indirect immunoperoxidase staining of viral proteins as well as by in situ hybridization of viral RNA with a [32P] -labeled probe and with a biotin-labeled probe.View full abstractDownload PDF (3514K) -
SAKAN MAEDA, HUI-LING CHEN, MITSUZOU HORIO, TAKETOSHI SUGIYAMA1988 Volume 21 Issue 2 Pages 165-173
Published: 1988
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (2278K) -
COMPARING WITH IMMUNOHISTOCHEMISTRY AND NORTHERN BLOT HYBRIDIZATIONKAZUO NAKANISHI, MASATO OKUDA, TAKESHI MAZAKI, TERUO MUKAINAKA, SETSUY ...1988 Volume 21 Issue 2 Pages 175-185
Published: 1988
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSIn situ hybridization using radioisotope-labeled cDNA probe was performed on cow brain. The cDNA fragment for glial fibrillary acidic protein (GFA-pro-tein) mRNA of cow was labeled with 32P-dCTP or 3H-dCTP by nick translation or random priming, and used as the probe. X-ray film macroautoradiograph of in situ hybridization using 32P-labeled probe revealed that the positive signals for GFA-protein mRNA were mainly located in the white matter of cow brain. By in situ hybridization using 3H-labeled probe, silver grains indicating existence of GFA-protein mRNA were detected on the cytoplasm of astroglia, but not on the nerve cells, vascular endothelial cells, choroid plexus and pia mater. The backgrounds were negligibly small. Specific grains were not observed on the control sections. These results were consistent with the findings of GFA-protein immunohistochemistry and Northern blot hybridization, indicating satisfactory specificity of in situ hybridization by the protocol presented here. The critical analysis of the specificity of hybridization-singles was thought to be the most important prerequisite in interpreting the result of in situ hybridization.View full abstractDownload PDF (3283K) -
TAKEHIKO KOJI, TETSUYA MORIUCHI, PAUL K. NAKANE1988 Volume 21 Issue 2 Pages 187-200
Published: 1988
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSTo better describe the physiological state of cells, detection of specific mRNA by in situ hybridization at the cellular level is often demanded. In order to accomplish this reliably, various factors such as morphological preservation, retention of mRNA, accessibility of the labeled probe to the target mRNA and efficiency of the hybridization should be considered during the tissue processing. In this paper, using non-radioactive probes, we investigated the effects of protease treatment and the size of probe on the efficiency of in situ hybridization with mRNAs in frozen and paraffin-embedded tissue sections of the rat pituitary glands. As non-radioactive haptenic DNA probes, we used dinitrophenyl (DNP) -labeled pro-opiomelanocortin (POMC) DNA and thymine-thymine (T-T) dimerized prolactin cDNA. The signals were visualized by the indirect enzyme-immunohis-tochemistry. The best results were obtained when frozen sections of tissues fixed by 4% paraformaldehyde in phosphate buffered saline were mildly digested with protease and hybridized with the haptenized DNAs of about 200-400 base pairs.View full abstractDownload PDF (3104K) -
TAKUSHI MONDEN, HIDEKI MORIMOTO, MASAHIKO HIGASHIYAMA, MASAHIRO MUROTA ...1988 Volume 21 Issue 2 Pages 201-210
Published: 1988
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSWe have previously reported the technique of in situ hybridization utilizing sulfonated DNA probes for the detection of amylase mRNA in formalin-fixed paraffin sections of human pancreas and submaxillary gland (22). Using this technique, we detected the mRNAs of Ha-ras and c-myc oncogenes in colonic cancer tissues from 24 patients, as well as in cultured cell lines. HL 60 cells, which are known to have amplified c-myc gene and accelerated transcription rate of this gene (11), and transformed NIH/3T3 cells with T24 Ha-ras were hybridized in situ with the sulfonated DNA probes of v-myc and v-Ha-ras respectively. Specific hybridization of v-myc probe for HL 60 and of v-Ha-ras probe for NIH/3T3 cells were clearly demonstrated at the cytoplasm by immunohistochemistry using a monoclonal antibody raised against sulfonated DNA. Of the colonic cancer tissues, which were freshly prepared and processed in paraffin, 6 were positive for Ha-ras mRNA and 7 were positive for c-myc mRNA. Northern blot analysis of the positive cases confirmed the validity of the results of in situ hybridization.View full abstractDownload PDF (2451K)
- |<
- <
- 1
- >
- >|