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Volume 7 (1974)
- Issue 4 Pages 259-
- Issue 3 Pages 181-
- Issue 2 Pages 105-
- Issue 1 Pages 1-
Volume 7, Issue 4
Displaying 1-10 of 10 articles from this issue
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KENJIRO YASUDA1974 Volume 7 Issue 4 Pages 259-264
Published: 1974
Released on J-STAGE: October 28, 2009
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ON THE IMMUNOFLUORESCENCE STUDY OF THE MOUSE LENS CRYSTALLINSAKIRA IKEDA1974 Volume 7 Issue 4 Pages 265-274
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSIn this study, I used an Olympus single beam microfluorimeter which was modified from the multi-purpose single beam microspectrophotometer according to my specifications.
This instrument was used to the sample the accuracy of localization of mouse lens crystallins by direct and indirect immunofluorescence methods. Some of the problems involved in microfluorimetry include: the necessity of the constant intensity of the exciting light, the choice of the filter system and the spot exciting method.
From a practical point of view it was concluded that the indirect method of immunofluorescence proivdes a more accurate localization of each lens crystallin than the direct method.View full abstractDownload PDF (6895K) -
TOSHIYUKI NAKATSUI, HARUTO UCHINO1974 Volume 7 Issue 4 Pages 275-281
Published: 1974
Released on J-STAGE: October 28, 2009
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MUNEAKI ABE, NOBUO OHUCHI, HIROYUKI SAKANO1974 Volume 7 Issue 4 Pages 282-288
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSSeen from the enzyme histochemical patterns, intestinal metaplasia appearing in the gastric mucosa was divided into two types; small intestine type and colon type. Among these types, the incidence of the former was highest. In the typical small intestine type, the patterns of the enzyme reactions in the striated border and cytoplasm were almost the same as in the jejunal villus epithelial cells. In addition, enzymaticall.y atypical cells among the small intestine type cells were frequently seen, too. In the colon type, activities of enzymes in the striated border were very weak or almost negative and cytoplasmic enzymes were moderately reactive resembling the pattern of the colon epithelial cells. In parallel with histochemical study, activities of disaccharidases were biochemically measured on the homogenate of the gastric mucosa. Following the appearance of intestinal metaplasia, activities of maltase and sucrase were significantly elevated showing the pattern of the small intestine, while lactase activity was not increased. In some cases, the ratios of maltase to sucrase activity were distributed in wider range in the intestinalized gastric mucosa than in the small intestine.
These enzymochemical findings in intestinal metaplasia were discussed from a viewpoint of cell proliferation and differentiation.View full abstractDownload PDF (4420K) -
YOSHIO SHIINA, BIN TAKEDA1974 Volume 7 Issue 4 Pages 289-304
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSFour kinds of hydrolytic enzymes, alkaline and acid phosphatases, leucine aminopeptidase and nonspecific esterase, were applied to cytological specimen for cytochemical diagnosis of carcinoma. Alkaline phosphatase is positive in normal columnar and reserve cells of uterine cervix, negative in normal squamous cells and irregularly positive, though decreased in reaction, in dysplasia and squamous carcinoma cells. This findings contribute to the idea of cytogenesis of cervical carcinoma of uterus as well as to practical cytological diagnosis. Four kinds of enzymes were also stained on the smear of metastatic effusion of gastric, pulmonary, ovarian, endometrial and mammary carcinoma. Cytochemical classification was designed to express peak and range of enzymatic distribution. Cytochemical patterns, thus represented, are instrumental to identify unknown primary site of tumor from ascitic and pleural fluid.View full abstractDownload PDF (11835K) -
YUTAKA MISHIMA1974 Volume 7 Issue 4 Pages 305-318
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe application of cytochemistry in clinical as well as basic medicine has been described with particular reference to pigmentary disorders which can be divided into three groups namely hyperfunction, hypofunction and neoplasm. For the hyperfunction of melanocytes which results in two types of hyperpigmentation, dermal and epidermal melanosome accumulation, the importance of cytochemical investigation of tyrosinase, and acid phosphatase at the cellular and subcellular level together with parallel biochemical enzyme assay has been presented. For the group of pigmentary disorders due to hypofunction of melanocytes the integral role of cytochemical findings such as dopa reaction, combined dopa-premelanin reactions, osmium iodide reaction and ATPase reaction has been described in relation to the differential diagnosis, prognosis and determination of therapeutic effects. For the third group of neoplastic pigmentary disorders, the clinical importance of dopa reaction and tyrosine reaction is mentioned in determining benign or malignant neo-plastic pigment cells. Furthermore the contribution of other enzyme cytochemistry such as butyryl and acetyl cholinesterase, development of combined dopa-acid phosphatase reaction, and thiamin pyrophosphatase reaction in the field of pigmentology from basic and clinical aspects has been presented with our recent findings at the cellular and subcellular level.View full abstractDownload PDF (30358K) -
ATSUSHI SUZUKI, HIDEO TAMATE1974 Volume 7 Issue 4 Pages 319-327
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSMuscle fibers in five different skeletal muscles of the steers were histochemically classified into five types. The type A reacted strongly for myosin ATPase and phosphorylase and moderately to strongly for succinate dehydrogenase (SDH) and NADH-diaphorase. The type B reacted strongly for myosin ATPase and phosphorylase and weakly for SDH and NADH-diaphorase. The type C reacted weakly for myosin ATPase, weakly to moderately for phosphorylase, and strongly for SDH and NADH-diaphorase. The type D reacted very weakly for myosin ATPase and phosphorylase, moderately to strongly for SDH, and strongly for NADH-diaphorase. The type E reacted moderately for myosin ATPase, weakly for phosphorylase, and very strongly for SDH and NADH-diaphorase. The β-hydroxybutyrate dehydrogenase (β-HBD) reaction was negative to very weak in the A fibers, negative in the B fibers, and very weak to moderate in the C fibers; it was moderate in the D fibers and very strong in the E fibers. The D fibers of the M. serratus ventralis were larger than four other types. The M. longissimus thoracis consisted of the A, B, and C fibers. The M. semitendinosus, M. serratus ventralis, and M. supraspinatus contained the A, B, C, and D fibers. The M. masseter consisted only of the E fibers.View full abstractDownload PDF (7145K) -
SHIGERU KATSURA, HIROSHI HORIBE, YUKIKO YOSHIDA, MASAOKI YAMADA1974 Volume 7 Issue 4 Pages 328-333
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThin-slices of human teeth were applied for the determination of ultraviolet absorption by using a microspectrophotometer (Zeiss UMSP type 1). The tooth tissue shows a non-specific ultraviolet absorption in a wide range of wave-length from 240nm to 300nm. The profile of spectra was different from that of nucleoprotein. The absorption spectrum in dentine was similar to that in cementum, but differ from that in enamel. The A260 and A280 show two or three times higher in dentine or enamel than those in cementum, respectively. A major part of ultraviolet absorbing substance in teeth seems to be extracted with a 5% of perchloric acid solution. A relative ultraviolet absorption value (A260 or A280 per unit area) distributed in different quantity relating to the tooth structure, whereas the value showed much higher in enamel than in dentine and lowest in cementum.View full abstractDownload PDF (449K) -
HIROSHI HORIBE, SHIGERU KATSURA, KEN FUJIMORI, MASAOKI YAMADA1974 Volume 7 Issue 4 Pages 334-341
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSFluorescence components were observed in thin-sections and extracts of human teeth by fluorometric methods. The fluorescence, being multiple, distributes in all the tooth tissue in different quality and quantity. An acidsoluble fraction (with a 5% perchloric acid) well corresponds in fluorescent components of dentine, cementum and enamel, while a fraction extracted with an alkaline ethylene-glycol is different in fluorescent composites between enamel and dentine, and the composites of dentine are quite similar to those of cementum. The relative fluorescence intensity at 530nm is the most intense in predentine, higher in dentine or cementum, and lower in enamel. The relative quantity seems less corresponding to ultraviolet absorption of tooth tissues.View full abstractDownload PDF (690K) -
TADAO MATSUURA, YOSHIAKI NOJYO, TAKASHI INOUE, YASUHIKO IBATA, YUTAKA ...1974 Volume 7 Issue 4 Pages 342-352
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe influence of 6-OHDA on the nigro-neostriatal dopaminergic system of the rat from birth to 45 days after birth was investigated with respect to the completion of the BBB to DA. The caudate nucleus and substantia nigra of infant rats were examined by Falck-Hillarp's fluorescence histochemistry and electron microscopy. The most pronounced decrease of DA fluorescence was detected in rats in whom 6-OHDA was repeatedly administered between the end of the first week to the middle of the second week after birth. DA fluorescence also began to decrease gradually in rats after administration of 6-OHDA in the middle of the second week. No further change, however, occurred in DA neurons of rats treated with 6-OHDA at the end of the second post natal week. It was determined that the BBB to DA began to be constituted from the middle of the second week after birth. We also conjectured that 6-OHDA had no potentiality to produce irreversible degeneration of DA neurons in infant animals, since DA fluorescence recovered to the same level as the controls in rats surviving for 4 to 6 weeks after administration of 6-OHDA during the first and second weeks after birth.View full abstractDownload PDF (13453K)
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