All issues
Volume 18 (1985)
- Issue 6 Pages 565-
- Issue 5 Pages 467-
- Issue 4 Pages 365-
- Issue 3 Pages 275-
- Issue 2 Pages 139-
- Issue 1 Pages 1-
Volume 18, Issue 3
Displaying 1-9 of 9 articles from this issue
- |<
- <
- 1
- >
- >|
-
IMMUNOHISTOCHEMICAL STUDY IN THE PARAFFIN SECTIONSAKIKO SETO-OHSHIMA, MAMORU SANO, AKIRA MIZUTANI1985 Volume 18 Issue 3 Pages 275-282
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA calcium-binding protein, calmodulin is thought to be an intracellular mediator for many calcium-dependent processes. In this report, to see the immunohistochemical localization of calmodulin in human tissues, routine paraffin sections were studied using either indirect immunoperoxidase or immunofluorescent method, and the characteristic staining was observed in some types of the epithelial cells, the neurons and others. After the preservation of the tissues in the formalin and in the paraffin sections, the immunoreactivity of calmodulin could still be demonstrated, which allows the accumulation of the knowledge concerning the pathological as well as normal distribution of calmodulin in human tissues for the elucidation of calmodulin functions in the human body.View full abstractDownload PDF (9835K) -
TOSHISUKE HIRAOKA, KEI-ICHI HIRAI, TADASHI UYEDA1985 Volume 18 Issue 3 Pages 283-292
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA new microspectrophotometric method for quantifying cytochrome oxidase activity has been developed based on a color-modified diaminobenzidine (DAB) reaction. The reaction consists of pretreating tissue sections or cell smears with 10mM cobalt acetate dissolved in 0.1M tris-HCl buffer of pH 7.4 for 10min at 37°C, washing and then treating with the DAB reagent for cytochrome oxidase for 15min at 37°C. Mitochondria turn reddish blue in the stained sections or smears. This reaction is enzymatic and conforms to Michaelis-Menten's formula. The microspectrophotometric quantification is effected by scanning the selected cytoplasmic area at 575nm or by measuring the cytoplasmic plug at two wavelengths, 575 and 665nm. As an example, the monomodal distribution in cytochrome oxidase activity was demonstrated among isolated rat liver cells by the two wavelength method.View full abstractDownload PDF (873K) -
HIDEAKI HASEGAWA, HIROSHI NAGURA, KEIICHI WATANABE1985 Volume 18 Issue 3 Pages 293-304
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSEndogenous peroxidases were histochemically demonstrated in epithelial cells over Peyer's patches in the jejunum of the rats. By one week after birth, the peroxidase-positive cells were found in the epithelial cells over the lymphoid aggregate in the jejunum. Those peroxidase-containing epithelial cells increased in number thereafter, while the peroxidase in those in the ileum remained negative. Electron microscopically the peroxidase was localized in the perinuclear spaces, cisternae of the endoplasmic reticulum and apically located cytoplasmic vesicles of the cell. The number of the peroxidase-positive cells in adult germ-free rats was much fewer than that in the conventional, although the distribution patterns of the peroxidase activity in the intestine were similar to each other. In point of the histochemical properties of the enzyme, this epithelial peroxidase appears to be analogous to the peroxidases of some other epithelial cells such as large intestine, salivary and lacrimal glands, and to differ from the myelo-peroxidase in the myeloid-derived cells.View full abstractDownload PDF (12215K) -
HIDEYUKI GOTO, MASAAKI MATSUSHIMA, TOSHIYUKI MARUYAMA, TAKASHI KIHARA1985 Volume 18 Issue 3 Pages 305-312
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe distribution of binding sites of two tritiated lectins, Concanavalin A (Con A) and wheat germ agglutinin (WGA), has been examined in mice by means of in vitro whole-body autoradiography, which is a powerful technique for localization of radioactive substances and enzymes in organs. Tritiated Con A exhibited a widespread distribution of binding sites in organs of the mouse. Tritiated Con A strongly bound to the stomach, rectum, cartilage and contents of the epididymis and deferent duct of all the organs tested. Tritiated WGA showed a restricted distribution of binding sites. Tritiated WGA vividly bound to the colon, cartilage and seminal vesicle of all the organs tested. In general, the present results are consistent with those obtained previously by microscopical studies.View full abstractDownload PDF (3758K) -
NAOKI OYAIZU, FUMIO HARA, AIRO TSUBURA, SOTOKICHI MORII, YUZURU KATO1985 Volume 18 Issue 3 Pages 313-322
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSEndogenous immunoreactive prolactin (PRL) in normal resting mammary gland and DMBA-induced mammary adenocarcinoma of female rats was demonstrated with the avidin-biotin-peroxidase complex (ABC) method. Positive staining obtained in the cytoplasm of glandular cells of the mammary gland were more intense on the apical borders of epithelial cells. The immunostaining in the induced tumors was variable, being often less than normal gland cells. The positivity could be seen only in the section fixed in Bouin's solution and treated with the ABC method using a rabbit antiserum against rat PRL. The immunostaining showed a strong positivity in the anterior pituitary tissue of the same animals, and it appeared specific for PRL because of its abolishment when the antiserum absorbed with rat PRL was used. The results reported here emphasize the value of ABC method in the Bouin's solution-fixed sections for detecting hormones in their target tissues.View full abstractDownload PDF (15197K) -
AN ULTRASTRUCTURAL STUDYKUNIO NAKAI, TAKUJI KASAMATSU1985 Volume 18 Issue 3 Pages 323-336
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSUsing early signs of terminal degeneration caused by direct injection of 6-hydroxydopamine (6-OHDA) as a specific marker for catecholamine (CA) terminal boutons, we studied the fine structure of these terminals in the glutaraldehyde-fixed visual cortex of adult cats. As previously observed in potassium permanganate (KMnO4)-fixed material, only a minority (29%) of the labeled CA boutons was found to be making synaptic contact. This low incidence of synapse-forming boutons was in sharp contrast to the high incidence exhibited by nonaminergic terminals in the same electron micrographs (58%). The former varied somewhat depending on the cortical layer examined, being the highest in layers II and III (40%), the lowest in layer I (22%) and intermediate in the bottom three layers (24-31%).
Dendrites and dendritic spines were the common target of synapse forming CA boutons, but a few axo-somatic contacts were also seen in layer VI. The CAergic synapses were of both types, symmetrical and asymmetrical, with a different frequency depending on the cortical layer. The symmetric type predominated in layers II, III and VI, while in layers IV and V and asymmetric synapses were as numerous as the symmetric ones. The present results further support the earlier suggestions that CA may be released from both synaptic and nonsynaptic axon terminals in the neocortex.View full abstractDownload PDF (13342K) -
ALFRED DORN, MICHAEL MÜLLER, HANS-GERT BERNSTEIN, ANTTI PAJUNEN, ...1985 Volume 18 Issue 3 Pages 337-342
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe distribution of L-ornithine decarboxylase was studied in developing rat kidneys by use of peroxidase-antiperoxidase technique. At postnatal day 2 the immunoreaction was exclusively localized in tubule cells. The glomeruli were devoid of immunostaining. A drastic decline of staining intensity occurred during the first two weeks of postnatal ontogenesis.View full abstractDownload PDF (3504K) -
TOSHIO HARIGAYA, KAZUMASA HOSHINO1985 Volume 18 Issue 3 Pages 343-352
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSSexual dimorphic changes in postnatal development of prolactin (PRL)-producing cells in the anterior pituitary gland in C57BL mice were immunohistochemically investigated. When examined at birth, PRL cells were already present in both male and female mice. With advancement of age, PRL cells increased in number. The shape of PRL cells at birth was oval, but on day 7 irregular-shaped PRL cells appeared. The shape of immunoreactive secretory granules in PRL cells were spherical at birth and also at day 7. However, on day 14 polymorphic PRL granules began to appear in type III PRL cells in both male and female mice. The ratios among type I, II, and III PRL cells and also the total number of all types of PRL cells were not different between male and female mice until 4 weeks of age. Meanwhile, type II PRL cells were predominant in both sexes. From 5 weeks of age, type III PRL cells began to increase in number in female mice only, thus sexual dimorphism in PRL cell types became noticeable during pubertal changes. The present results suggest that postnatal morphological, particularly sexual dimorphic, changes observed in secretory granules as well as cell shapes of PRL cells are mainly due to the reflection of endocrine functions in pubertal female mice.View full abstractDownload PDF (11624K) -
YOSHIAKI TAKAI, YOHKO NODA, SHINICHIRO SUMITOMO, KENICHI KAWAMURA, MAS ...1985 Volume 18 Issue 3 Pages 353-361
Published: 1985
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSImmunohistochemical identification of keratin proteins in ductal segments was reported in salivary glands of the hamster, guinea pig, dog, horse, pig, cow, goat, monkey and human. The keratin found in salivary gland ducts was usually located in the luminal cytoplasms of the striated duct (SD) epithelium in comparatively high concentrations. The luminal cytoplasm of intercalated duct (ICD) cells also displayed a high level of keratin staining. The excretory duct (ED) cells were positive for keratin in the inner side cytoplasm of the epithelium. The SD epithelium of the guinea pig submandibular gland was characterized by the presence of negative or positive keratin staining. The cells in the outer layer of large EDs in mammals salivary glands disclosed the highest degree of keratin staining.View full abstractDownload PDF (10856K)
- |<
- <
- 1
- >
- >|