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Volume 23 (1990)
- Issue 6 Pages 753-
- Issue 5 Pages 601-
- Issue 4 Pages 427-
- Issue 3 Pages 261-
- Issue 2 Pages 139-
- Issue 1 Pages 1-
Volume 23, Issue 2
Displaying 1-12 of 12 articles from this issue
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KOZO YONEDA, TOYOSHI FUJIMOTO, SADAO IMAMURA, KAZUO OGAWA1990 Volume 23 Issue 2 Pages 139-148
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDistribution of fodrin in a mouse keratinocyte cell line, Pam 212, was studied by two novel techniques of immunogold electron microscopy. Firstly, by utilizing ultra-small (1 nm) gold-conjugated antibody and silver enhancement in the preembedding procedure, the difference of fodrin distribution in three culture conditions was shown in a semiquantitative manner. Fodrin was observed throughout the cytoplasm in cells cultured in low calcium (LC: 0.06 mM) medium, but it was concentrated beneath the plasma membrane in cells in standard calcium (SC: 1.87 mM); when cells in LC medium were treated with 10ng/ml 12-o-tetradecanoylphorbol-13-acetate (TPA) for two hr, the protein became dense near the plasma membrane. Secondly, by treating the immunogold-labeled sample by the dry-blowing method (Araki, N. and Ogawa, K.: Acta histochem. cytochem. 19; 31-37, 1986), fodrin was observed along actin-like microfilaments in Pam 212 cells in LC medium. After treatment with 30 μM cytochalasin B for one hr, the labeling became confined to an amorphous substance which is likely to be composed of depolymerized actin.
The present study showed the distributional change of fodrin at the ultrastructural level: in LC cells fodrin was mostly in the cytoplasm and at least some were associated with actin filaments; ‘calcium switch’ produced its redistribution to the plasma membrane. In addition, since TPA was revealed to cause a similar result as ‘calcium switch’, phosphorylation of fodrin molecule might be causally related to the rearrangement.View full abstractDownload PDF (5124K) -
JIAN-ZHAO NIU, SHUJI YAMASHITA, KENJIRO YASUDA1990 Volume 23 Issue 2 Pages 149-163
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSGamma-glutamyl transpeptidase (γ-GTP) was localized in prenatal and neonatal rat kidneys using an immunohistochemical method with monoclonal antibodies to rat kidney γ-GTP. γ-GTP was demonstrated on the brush border and basolateral membrane of proximal convoluted tubules from the 18th fetal day. The basolateral membrane of proximal tubules revealed strong reaction at 1 and 3 days after birth. The reaction of basolateral membrane decreased with the maturation of nephrons, however, moderate reaction was still observed at 30 days of age in the S3 segment. Throughout the postnatal development of the kidney, the S3 exhibited the strongest immunostainingand S1 showed the weakest reaction, and proximal tubules in the deep layer of the cortex always provided a stronger reaction than those in the superficial layer of the cortex. Maturating cuboidal and squamous epithelial cells of descending Henle's loop showed a positive reaction on the luminal and lateral cell membranes. On immunoelectron microscopy, γ-GTP was found on the brush border, basolateral membrane, membranes of pinocytotic vesicles and apical invaginations in proximal tubular cells at 1-7 days after birth. However, tight junctions were devoid of the reaction. The development of basal infolding and localization of γ-GTP there seemed to relate closely to the development of surrounding capillaries. γ-GTP was localized on the cell membrane and occasionally in the cytoplasm of interstitial cells of renal interstitium.View full abstractDownload PDF (12244K) -
YOSHIFUMI HIRABAYASHI, TAKANORI SAKAGAMI, KAZUYORI YAMADA1990 Volume 23 Issue 2 Pages 165-173
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSPostembedding staining methods using ruthenium red (RR) incombination with tungstate (TT) (phospho- or silicotungstic acid) (RR-TT) were tested for the effective visualization of acidic glycoconjugates in electron microscopy. Tissue blocks of trachea and colon from adult male Wistar rats were fixed in phosphatebuffered glutaraldehyde-paraformaldehyde solution and embedded in LR-White. Ultrathin sections were cut, stained first with RR (pH 2.5) and then incubated in TT. In the tissues examined, all the ultrastructures containing acidic glycoconjugates exhibited apparent positive reaction for RR-TT. If stained singly with RR or TT under the same staining conditions, most of these ultrastructures failed to give any distinct electron densities. In all the acidic glycoconjugate-containing ultrastructures tested, digestion with testicular hyaluronidase greatly diminished or abolished their RR-TT reactions. In view of these results, the present RR-TT methods can be regarded as a useful technique for the efficient visualization of acidic glycoconjugates in electron microscopy.View full abstractDownload PDF (5659K) -
OBSERVATION BY LIGHT AND ELECTRON MICROSCOPE AND BY ENZYME HISTOCYTOCHEMISTRYYUXIU SHI, LI LIU, WEI HE, YAN WANG, DONGJIAN LIU, WAN QI, ZHANYOU WAN ...1990 Volume 23 Issue 2 Pages 175-187
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSIn the present study, we have done an acute toxicological experiment on the rat liver by administration of SVATE-3 in clinical doses (high and low dose, respectively) for 3 weeks. By using morphological methods, light and electron microscopy, enzyme histocytochemistry in light and electron microscope and biochemistry, we have observed the histological structure and ultrastructure of rat livers as well as the activity and distribution of the index enzymes for the liver cell membrane such as alkaline phosphatase, 5′-nucleotidase, Mg-adenosine triphosphatase and glucose-6-phosphatase for liver cell organelle: endoplasmic reticulum. Furthermore, the biochemical quantitation has been given. The results show that there is no toxic damage to the histological structure of rat liver by administration of SVATE-3 in the acute toxicological experiments. There is no obvious change of histocytochemical enzyme activity and distribution of these 4 types of index enzymes. All these suggest that the application of SVATE-3 within clinical doses shows no toxic damage to rat liver.View full abstractDownload PDF (5302K) -
ARSENIA SCOTTI DE CAROLIS, MARIA GRAZIA CAPORALI, TARCISIO NIGLIO, ALB ...1990 Volume 23 Issue 2 Pages 189-194
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSBiochemical and behavioral studies demonstrated lateralized differences in several areas of the rat limbic system. However, no significant right-left differences in several morphological parameters of the hippocampus were reported in some anatomical studies. To evaluate further possible asymmetries in microanatomy of the rat hippocampus the intensity of Timmstaining in different hippocampal fields and the area occupied by mossy fibres were assessed using combined microdensitometric and quantitative image analysis techniques. Timmstaining is a sensitive marker for the histochemical detection of zinc and other heavy transition metals and it is considered to be related to the distribution of intrahippocampal association pathways. No right-left differences were seen in the density of Timm-staining at the level of the dentate gyrus and in the dendritic layer of CA1 and Ca2 fields, in the mossy fibre area or in the subiculum. These findings support the view of lack of morphologic asymmetry in the rat hippocampus.View full abstractDownload PDF (1991K) -
MASANORI MURAKOSHI, RIE INADA, R. MINORU SUZUKI, KEI-ICHI WATANABE1990 Volume 23 Issue 2 Pages 195-202
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSImmunocytochemical localization of glutathione-peroxidase (GSH-PO) in the stomach was studied in rats under the following experimental conditions; 1) normal untreated 2) histamine-administration 3) atropine-administration in order to clarify the role of GSH-PO. In normal untreated group, GSH-PO was mainly observed in the central part of the gland. At higher magnification, GSH-PO was diffusely distributed in the pyramidal or oval-shaped cytoplasm. Intracellular localization of GSH-PO was predominantly observed in the cytoplasm of the parietal cells. In histamine-administered animal, GSH-PO was intensely stained in cytoplasm of the parietal cells with well-developed secretory canaliculi. In atropine-administered animal, the intensity of the GSH-PO staining in the parietal cells was remarkably decreased. By immunoelectron microscopic investigations, GSH-PO was noted in cytoplasm as small granules or lysosome-like structures.
Based on our data, it is suggested that GSH-PO in the gastric parietal cells may play an important role in the prevention of damage to the microsomes with lipid peroxides induced during the process of HCl secretion. We further speculated that GSH-PO within the lysosome-like structures after atrophic-administration might be a result of the degradation process of the peroxidized materials.View full abstractDownload PDF (5036K) -
HANS-GERT BERNSTEIN, HEIDRUN KIRSCHKE, THOMAS ROSKODEN, BERND WIEDERAN ...1990 Volume 23 Issue 2 Pages 203-207
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSImmunohistochemically detectable cathepsin L was demonstrated in rat brain by means of a monospecific, polyclonal antiserum. Sternberger's unlabeled immunoenzyme technique was employed. Cathepsin L immunoreactive material was found in multiple hypothalamic and brain stem neurocytes as well as in some hippocampal and neocortical nerve cells. Cathepsin L was also detected in ependymal cells of the plexus choroideus.View full abstractDownload PDF (2717K) -
AKIHIDE KAMEGAI, YASUNORI MURAMATSU, TOSHI-ICHIRO TANABE, HIROMITU MOR ...1990 Volume 23 Issue 2 Pages 209-218
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSChondroidal tissue induced by electric stimulation in rat tibial periosteum or by bone morphogenetic protein (BMP) implanted in mouse thigh muscle was examined immunohistochemically for S-100 protein with the use of polyclonal antiserum (S-100) and monoclonal antibodies to S-100 protein subunits α and β (S-100α and S-100β). Chondrocytes were occasionally found in the electric callus (EC), and ossification induced by BMP began from chondroidal tissue in the muscle. All the types of cartilage cells displayed strong staining for S-100 and S-100α subunit, and slight staining for S-100β. Bone cells such as osteocytes and osteoclasts appearing after chondrogenesis were generally devoid of S-100 protein. During bone formation, the immunoreaction for S-100 protein was characteristically restricted to cartilage cells. The presence of S-100 protein throughout chondrogenesis suggests that this protein might participate in calciumprotein interactions occurring during cartilage formation.View full abstractDownload PDF (5381K) -
PHILIPPE ANGLADE, SHIGERU TSUJI1990 Volume 23 Issue 2 Pages 219-226
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSA method was devised for the simultaneous localization of neuropeptides and acetylcholine (ACh) -like cation at ultrastructural level in a paraformaldehyde fixed nervous tissue (myenteric plexus). In order to minimize the loss of tissual ACh, no membrane permeabilization was made before the immunocytochemical reaction. Conventional pre-embedding immunoperoxidase reaction for neuropeptides detection [substance P and vasoactive intestinal polypeptide (VIP)] was followed by cytochemical ionic fixation of ACh-like cation. All the substance P- or VIP-immunoreactive nerve endings possessed punctiform precipitates of ACh-like cations situated in the small clear synaptic vesicles (30-50 nm). In the vicinity of substance P- and VIP-immunoreactive nerve terminals, some other nerve terminals containing ACh-like cations did not contain any peptide immunoreactivity.View full abstractDownload PDF (3770K) -
SHINJI MATSUTANI, NOBORU YAMAMOTO1990 Volume 23 Issue 2 Pages 227-236
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSFor immuno-electron-microscopic observations of cultured cells, we used a novel substrate, a commonly used domestic wrapping film, for plating cells, and irradiated microwave (MW) during fixation or during post-embedding immunoreaction. Isolated hepatocytes cultured on wrapping film could be observed under a phase-contrast microscope, appeared healthy, and were sectioned in various orientations for electron microscopy. Post-embedding immunogold staining was carried out on these sections, and the density of gold particles was used to estimate the effects of MW irradiation. Although MW irradiation (60 sec) during fixation brought about a comparable density to that of conventional fixation (15 min immersion), the preservation of fine structures was poorer in irradiated specimens. The time required for the reaction with primary antibody was reduced with MW irradiation. Sixty-second irradiation was shown to give an equivalent result to overnight reaction without irradiation.View full abstractDownload PDF (6786K) -
YOUNG-CHI KIM, YOSHIO WAKATSUKI, KATSUHIKO OGAWA, YASUAKI NAKASHIMA, K ...1990 Volume 23 Issue 2 Pages 237-244
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSR-binder is one of the binding proteins for vitamin B12. It appears to inhibit bacterial growth by competing for the vitamin with bacteria. We investigated the localization of R-binder in human fallopian tube immunohistochemically. Positive staining for R-binder was seen in epithelium of their isthmus (8 of 19 cases, 42%) and ampulla (18 of 27 cases, 67%). Staining in the nonciliated secretory cells was seen in the apical membranes with frequent and intense cytoplasmic immunoreactivity. The ciliated cells showed the staining mainly in the cilia. Tubal fluid was also positive for R-binder. The data suggest that R-binder plays a role in local defense mechanisms in the fallopian tube as in other organs.View full abstractDownload PDF (3128K) -
Setsuya Fujita, Tsukasa Ashihara, TIBOR BARKA1990 Volume 23 Issue 2 Pages 245-255
Published: 1990
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (1807K)
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