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Volume 15 (1982)
- Issue 6 Pages 717-
- Issue 5 Pages 639-
- Issue 4 Pages 489-
- Issue 3 Pages 313-
- Issue 1-2 Pages 5-
Volume 15, Issue 3
Displaying 1-12 of 12 articles from this issue
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HIROKO YOKOTA, KAZUO OGAWA1982 Volume 15 Issue 3 Pages 313-324
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSIn our previous reports we discussed the morphological changes in the cellular and acellular components of the renal glomerulus occuring in an experimental congestive kidney model using rabbits. In the present investigation, the second of a series, the changes in the glomerular basement membrane and mesangium at 6-7 months after the congestive procedure were observed by using the following cytochemical methods: ruthenium red (RR) staining, periodic acid-silver methenamine (PAM) staining, performic acid-phosphotungstic acid (PFP) reaction, and dextran tracer.
In both control and congestive groups, the basement membrane and the masangial matrix were intensely stained with RR and PAM, and slightly stained with PFP. RR-stained particles, which indicate anionic sites, were observed to have a quasiregular pattern in the basement membrane and the mesangial matrix in both the control and congestive groups. The distribution pattern of infused dextran in the mesangium of the congestive kidney was essentially similar to that of the control. In conclusion, the altered acellular components in the congestive kidney are cytochemically similar to those of the control acellular component proper.View full abstractDownload PDF (19581K) -
KAZUSHI FUJIMOTO, KAZUO OGAWA1982 Volume 15 Issue 3 Pages 325-337
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe cytochemical localization of adenylate cyclase and guanylate cyclase in rat cardiac muscle was demonstrated with the lead nitrate method using dimethyl sulfoxide (DMSO) (Fujimoto et al., 1981). Tissues were fixed in a mixture of 2% paraformaldehyde, 0.25% glutaraldehyde and 5% DMSO in 0.1M cacodylate buffer, pH7.4, for 30min, and then washed with 5% DMSO in 0.1M cacodylate buffer. Vibratome or Microslicer sections were incubated in the following medium for 30min at 37°C.
Medium for adenylate cyclase (ACLase): 80mM Tris-maleate buffer, pH 7.4; 4mM MgSO4; 10mM NaF; 2mM theophylline; 2mM lead nitrate; 0.25M sucrose; 5% DMSO; 2.5mM levamisole; 0.5mM AMP-PNP. Medium for guanylate cyclase (GCLase): 80mM Tris-maleate buffer, pH 7.4; 3mM MnCl2; 2mM theophylline; 2mM lead nitrate; 0.25M sucrose; 5% DMSO; 2.5mM levamisole; 0.5mM GMP-PNP. The most intense reaction of ACLase was localized in the sarcolemma, sarcoplasmic reticulum and gap junction. GCLase activity was also seen in the same regions. In the unfixed isolated gap junctions, the reaction product was seen to interrupt the interspace of the junction, forming bridges between the junctional membranes. ACLase was activated by NaF and isoproterenol. Alloxan was effective as an inhibitor of ACLase. GCLase was stimulated by the in vitro acetylcholine treatment.View full abstractDownload PDF (18119K) -
KAZUSHI FUJIMOTO, KAZUO OGAWA1982 Volume 15 Issue 3 Pages 338-354
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSMyocardial Ca++-activated adenosine triphosphatase (Ca++-ATPase) and ouabain-sensitive, K+-dependent p-nitrophenylphosphatase (K+-NPPase) were demonstrated by the method of Ando et al. (1981) and Mayahara et al. (1980). At pH 9.0, Ca++-ATPase activity was localized on the myofilaments, sarcoplasmic reticulum, gap junction, and the matrix of mitochondria. Sarcolemmal ATPase activity could be detected at neutral pH. K+-NPPase was seen on the cytoplasmic side of the sarcolemma and T-tubule. These findings support the role of these structures for intracellular Ca++-Na+ (probably K+ also related) interaction inducing the heart activity.View full abstractDownload PDF (31527K) -
MART NAKAHARA, HAJIME SUGIHARA, SHINICHI MIYABARA, KAZUHIRO OOTA, KANK ...1982 Volume 15 Issue 3 Pages 355-361
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe development and differentiation of the fetal adrenal cortex was studied in the rat by lipid and enzyme histochemistry.
The adrenal cortex began to have small amounts of lipid droplets at 171/2 days by oil red O stain and the amounts increased gradually. Cholesterol by Schultz reaction became clear at 201/2 days. At 151/2 days weak activity of glucose 6-phosphate dehydrogenase (G-6-PDH) was already present in the inner cortex, which was followed a day later by weakly positive reaction of 3β-hydroxysteroid dehydrogenase (3β-HSD). At 181/2 days positive activity of secondary alcohol dehydrogenase (SAD) was obvious in the inner cortex. Activity of alkaline phosphatase (Al-Pase) was observed in the subcapsular zone from day 181/2 on. Enzymatic activities of the cortex increased gradually after their appearance as development proceeded. These findings show that histochemical activities of such principal enzymes as G-6-PDH and 3β-HSD precede appearance of lipid droplets.
As a conclusion, zona reticularis is not distinguishable from zona fasciculata during the fetal stage, zona fasciculo-reticularis differentiates histochemically in a 171/2 day old fetus, and finally differentiation of zona glomerulosa occurs at 201/2 days in gestation.View full abstractDownload PDF (3363K) -
ATSUSHI SUZUKI, TAKESHI TSUCHIYA, HIDEO TAMATE1982 Volume 15 Issue 3 Pages 362-371
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe presence of subtypes of dark and light myofibers distinguished by myosin adenosine triphosphatase (ATPase) reaction after alkaline preincubation was studied in thigh muscles of chickens. The dark myofibers were subclassified into type II and SS, and the light myofibers into type I, SM, and MM myofibers by the pH sensitivity of myosin ATPase. Type I myofibers reacted strongly for acid-stable ATPase and weakly for alkali-stable ATPase. Type II myofibers reacted negatively for acid-stable ATPase and strongly for alkali-stable ATPase. Type SM myofibers reacted strongly for acid-stable ATPase and modeiately for alkali-stable ATPase. Type SS myofibers reacted strongly and type MM myofibers reacted moderately for both acid-stable and alkali-stable ATPase. Type II myofibers were subdivided into type IIA with strong activity for NADH: Nitro BT oxidoreductase (NADHOX) and type IIB with weak NADHOX activity. Type IIB myofibers were strong in activity for glycerol-3-phosphate: menadione oxidoreductase (GPOX) whereas many type IIA myofibers were weak. Type IIA myofibers were stained weakly to moderately with 3-hydroxybutyrate: NAD+ oxidoreductase (HBOX) and type IIB myofibers were stained very weakly. Type I, SM, SS, and MM myofibers were stained strongly with NADHOX, weakly to moderately with HBOX, and weakly with GPOX.View full abstractDownload PDF (9425K) -
YOSHIMARO ISHIKAWA, KEI-ICHI HIRAI, KAZUO OGAWA1982 Volume 15 Issue 3 Pages 372-378
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA glutaraldehyde-insensitive, NAD(P)H-dependent H2O2-generating enzyme activity in the rat small intestine and its regional differences in the small intestine of the adult rat have been described previously by us (5, 6). In the present investigation the postnatal changes of this enzyme activity in the small intestine were studied using 1-day to 6-months old male Kyo-Wistar rats.
It was found in this study that the NAD(P)H-dependent H2O2-generating enzyme activity showed a marked postnatal change. The enzyme activity was significantly higher in the apical membrane of absorptive cells of both duodenojejunum and ileum in newborn rats, however the activity in the apical membrane of the ileum was decreased from the 7th postnatal day on, reaching eventually to an extremely weak activity in the adult. The activity in the apical membrane of the duodeno-jejunum remained the same throughout the course of growth. These findings are discussed in connection with the postnatal changes in absorptive function.View full abstractDownload PDF (11884K) -
YOSHIMARO ISHIKAWA, KEI-ICHI HIRAI, KAZUO OGAWA1982 Volume 15 Issue 3 Pages 379-385
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSRegional differences of an NAD(P)H-dependent H2O2-generating enzyme activity in the rat small intestinal mucosa were electron-cytochemically investigated. The H2O2-generating activity was localized on the apical membrane covering microvilli of intestinal absorptive cells in the duodeno-jejunum. The activity was weak in absorptive cells at the mitotic area of villus, becoming higher toward its apex. The spherical membrane covering absorptive cells which are in the process of being extruded at the apical extrusion zone exhibited the activity. Activity was also noted in the apical cell membrane of goblet cells, although the activity was weaker than that in the absorptive cells.
The ileal activity was apparently weaker than that of the duodeno-jejunum. These results seem to indicate that there are local variations in the NAD(P)H-depending H2O2-generating activity among intestinal epithelial cells.View full abstractDownload PDF (12351K) -
SPECIAL REFERENCE TO NON-FUNCTIONING TUMORS (ADENOMA AND ADENOMATOUS NODULE) AND FUNCTIONING ADENOMAKAZUTO SHIGEMATSU1982 Volume 15 Issue 3 Pages 386-400
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe hormone content in Cushing-type non-functioning tumors of the adrenal glands was measured to be compared with those of functioning adenomas. Furthermore, the relationship between the hormone contents and the proportion of clear-type cells and compact-type cells in the tissues were also studied.
The contents of aldosterone and corticosterone were significantly higher in primary aldosteronism than in other groups. The cortisol content was significantly higher in Cushing's syndrome than in primary aldosteronism, but it was not statistically different from non-functioning adenomatous nodules. The patterns of hormone contents in non-functioning adenomatous nodules were similar to those in Cushing's syndrome.
In primary aldosteronism, there was a positive correlation between the aldosterone content and the increase of compact-type cells. Compact-type cells revealed high activities of 3β-hydroxysteroid dehydrogenase and glucose 6-phosphate dehydrogenase. These results suggest that compact-type cells play an important role in synthesis and secretion of aldosterone in primary aldosteronism.
In Cushing's syndrome, there was no significant correlation between the cortisol content and the proportion of constituting cells. The activities of 3βHSD and G6PD in both types of cells were not so different from each other. Therefore, there may be a possibility that morphological differences in the two types of constituting cells show different phases of tumor cells in Cushing's syndrome.
In the non-functioning tumors, the scattered tumor cells revealed marked activities of 3βHSD and G6PD. These results suggest that the number of constituting cells of “non-functioning” tumors have some degree of endocrinologic activity.View full abstractDownload PDF (3872K) -
OSAMU MORI1982 Volume 15 Issue 3 Pages 401-410
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA cytofluorometric evaluation of the DNA content of the bone and soft part tumor cell nuclei was undertaken in 14 cases of benign tumors and 28 cases of malignant tumors. In benign tumors, the DNA values were in the diploidtetraploid range and there were no tumor cells which had more DNA value than the octaploid. On the contrary, malignant tumors were characterized by the prevalence of polyploid cells.
The study suggests that DNA cytofluorometry is useful for differentiating between malignant and benign tumors in the bone and soft part tissues.View full abstractDownload PDF (894K) -
TOSHITAKA AKISAKA, ICHIJI IMANISHI, HIROSHI INOUE1982 Volume 15 Issue 3 Pages 411-420
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSEmbryonic Meckel's cartilage cells, following prolonged osmication, exhibited complete impregnation of the Golgi apparatus, endoplasmic reticulum (ER), nuclear envelopes, mitochondria, multivesicular bodies and some matrix vesicles. Most of the Golgi apparatus showed a specific localization of the reaction product. However, no deposition occured on the plasma membrane, in the cellular matrix and cartilaginous matrix. Osmium deposits were restricted within some type of matrix vesicles at the extracellular space. As to the stainability for the matrix vesicles, two different types were identified: one contained the reaction product, and the other was free of it. These results suggested that the intracytoplasmic organelles showing osmium-positive reaction might be involved in some way in the formation of matrix vesicles.View full abstractDownload PDF (22585K) -
HIROSHI MAYAHARA, TAKAO ANDO, YOSHIMARO ISHIKAWA, Kazuo OGAWA1982 Volume 15 Issue 3 Pages 421-438
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe ultracytochemical localization of ouabain-sensitive, K-dependent p-nitrophenylphosphatase (K-NPPase) activity of Na-K-ATPase was investigated in the fetal and neonatal rat kidneys with the one-step lead citrate method (17) in order to study the developmental changes in the site of the Na-pump.
In fetal rat kidneys, the K-NPPase positive renal tubules first appeared in the inner cortex at 18 gestation days and increased with the maturation of the fetus. Electron microscopically, the reaction products were found on the basolateral plasma membranes of distal convoluted tubule cells at various levels of differentiation. In the kidney of 19 and more gestation days, K-NPPase positive tubules were found also in the ascending thick tubules of the loop of Henle in the medulla. In newborn rats, some of the ascending thick tubules with intense K-NPPase activity reached to the tip of the papilla. These tubules in the papilla gradually lost K-NPPase activity and redifferentiated into ascending thin tubules during the period of lactation. The clear separation of K-NPPase positive outer medulla and K-NPPase negative inner medulla was completed around 3 weeks after birth. The above results indicate that suckling rats have Na-pumps even in the inner medulla and thus have different urine concentration mechanisms from those in the adults.View full abstractDownload PDF (35214K) -
1982 Volume 15 Issue 3 Pages 439-484
Published: 1982
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSDownload PDF (7067K)
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