We investigated the expression of
c-jun and
c-fos proto-oncogenes and their distribution in the brain of newborn to adult rats to determine the relationship between protooncogene expression and brain development, by the reverse transcription-polymerase chain rection (RT-PCR) method,
in situ hybridization and immunohistochemistry. In the RT-PCR analysis, the cDNA fragments of
c-jun and
c-fos were amplified with expected-sizes, and the expression levels were fixed through all ages examined. The
in situ hybridization showed that the expression of
c-jun and
c-fos mRNA was located at the cytoplasm of most neurons and some glial cells of the entire portions of the cerebrum, brainstem and cerebellum until 300 days after birth. c-jun protein immunoreactivity was detected in the nucleus of neurons which demonstrated a positive signal by
in situ hybridization. Our results suggest that even after neuroblasts differentiate to mature neurons,
c-jun and
c-fos genes may have a role in the preservation of the neuronal function of the rat central nervous system.
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