ACTA HISTOCHEMICA ET CYTOCHEMICA

THE USE OF VIBRATOME IN ULTRACYTOCHEMISTRY

YASUHIRO AGO, KAZUO OGAWA

1973 Volume 6 Issue 1 Pages 1-10
Published: 1973
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.6.1

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SCANNING ELECTRON MICROSCOPIC OBSERVATIONS OF BIOLOGICAL SECTIONS CUT ON A “VIBRATOME” AND INCUBATED IN MEDIA FOR ATP-ASE AND ALP-ASE ACTIVITY

SEM APPEARANCE OF THE VIBRATOME SECTIONS

TAKASHI MAKITA

1973 Volume 6 Issue 1 Pages 11-20
Published: 1973
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.6.11

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The suitability of non-frozen sections, cut with a Vibratome, for cytochemistry was examined with a scanning electron microscope.
Sections, 10μ to 50μ in thickness, were prepared from unfixed tissue or tissue fixed with perfusion of glutaraldehyde solution. They were incubated in Wachstein-Meisel medium for ATPase or in Mayahara-Ogawa medium for ALPase. The sections were dehydrated and dried under vacuum or with the critical point drying method.
The surface of sections prepared from fresh tissue which was fixed after sectioning often was coated with blood cells and extracellular fluid.
Judging from the SEM appearance, the Vibratome sections were comparatively uniform in thickness but had fine chatter marks on their surface while sections cut on a Tissue Sectioner were irregular in thickness but provided topographic areas where SEM examination was not imparied by knife marks.
Deposits of lead-phosphate were conspicuous on the surface of the incubated tissue from duodenum but not on the cut surface of the section. Irregularly distributed aggregates of precipitate were also a common finding on the surface of the tissue but not on the cut surface.

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ULTRACYTOCHEMISTRY OF MITOSIS

TAKUMA SAITO

1973 Volume 6 Issue 1 Pages 21-26
Published: 1973
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.6.21

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The changes of the organelles during mitosis were observed in hepatic parenchymal cells after partial hepatectomy. The endoplasmic reticulum plays a roll to form nuclear envelope of dauthter nuclei at early telophase. The Golgi apparatus shows a dynamic morphological changes during mitosis. The number of the peroxisomes decreased before the onset of first cell division. The marker enzymes of those organelles, glucose-6-phosphatase for endoplasmic reticulum, thiamine pyrophosphatase for Golgi apparatus, Peroxidase for peroxisome, helps great deal to identify the organelles especially which show dynamic changes of morphology.

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AN APPLICATION OF ELECTRON MICROSCOPIC CYTOCHEMISTRY TO HUMAN TUMORS

MASAO YOKOYAMA

1973 Volume 6 Issue 1 Pages 27-34
Published: 1973
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.6.27

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Prostatic carcinoma, renal cell carcinoma and melanoma were studied by electron microscopic enzyme cytochemistry. ACPase of prostatic cancer cells was found in lysosomes, secretory vacuoles and vesicles as well as some Golgi apparatuses. The enzyme localizations remained unchanged in tumor cells two weeks after castration when cells were viable. Renal cell carcinomas revealed to have positive ALPase activity at microvilli resembling brush border and lateral plasma membranes facing adjacent tumor cells. The enzyme was negative at basal plasma membrane of the tumor cells. Proximal tubule cells of human kidney showed ALPase activity only at apical membranes. This indicated functional dedifferentiation of plasma membrane in the tumor cells. These observations were verified and substantiated by TPPase, IDPase and G6Pase localizations in tumor cells. The tumor cells also showed positive G6Pase in endoplasmic reticulum. DOPA oxidase was successfully detected in smooth endoplasmic reticulum close to Golgi apparatus, innermost Golgi cisternae and premelanosomes both in cultured melanoma cells and melanoma tissues, which may have diagnostic value. Thus, EM enzyme cytochemistry was considered to be applicable and useful for human cancer study concerning histogenesis, diagnosis and to assess the treatment.

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RECENT ADVANCE IN THE FIELD OF ELECTRON MICROSCOPIC IMMUNOCYTOCHEMISTRY

KENJIRO YASUDA

1973 Volume 6 Issue 1 Pages 35-38
Published: 1973
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.6.35

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ATTEMPTS TO STAIN THE INTRACELLULAR ANTIGENS BY MEANS OF IMMUNO-FERRITIN METHOD

HIROSHI HIRANO

1973 Volume 6 Issue 1 Pages 39-43
Published: 1973
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.6.39

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DEMONSTRATION OF THE ELEMENTAL DISTRIBUTION IN BIOLOGICAL TISSUES BY MEANS OF THE ELECTRON MICROSCOPE AND ELECTRON PROBE X-RAY MICROANALYZER

VINCI MIZUHIRA

1973 Volume 6 Issue 1 Pages 44-52
Published: 1973
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.6.44

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CONCLUDING REMARKS; THE FUTURE OF ULTRACYTOCHEMISTRY

KAZUO OGAWA

1973 Volume 6 Issue 1 Pages 53-54
Published: 1973
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.6.53

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Structure of Macromolecular Glycogen Particles and Intracellular Synthesis of Macromolecular Polyglucoses

Tadao TAKEUCHI

1973 Volume 6 Issue 1 Pages 55-56
Published: 1973
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.6.55

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GENERAL SESSION

1973 Volume 6 Issue 1 Pages 56-84
Published: 1973
Released on J-STAGE: October 28, 2009

DOIhttps://doi.org/10.1267/ahc.6.56

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