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Volume 6 (1973)
- Issue 4 Pages 233-
- Issue 3 Pages 151-
- Issue 2 Pages 89-
- Issue 1 Pages 1-
Volume 6, Issue 2
Displaying 1-7 of 7 articles from this issue
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II. HISTOCHEMICAL OBSERVATION ON THE SECRETIONS OF THE LUMINAL SURFACES AND THE GLANDULAR REGIONSYOSHITERU KATZUKURA, HIDEO TAMATE1973 Volume 6 Issue 2 Pages 89-97
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSUsing ten hens of white Leghorn 150 days after hatching, samplings were carried out from their oviducts at various stages of laying or non-laying hens, which were defined in the previous study (7). Histochemical observation of the mucosubstances in the luminal epithelia and gland cells in the lamina propria were performed.
The infundibulum has been known as the chalaziferous gland. However, the present study revealed that the secretion materials from the luminal epithelium were sulfomucins (chondroitin sulfate C) and sialo- or hyaluromucins which constituted the chalaza in the lumen together with those from the magnum. PAS-positive mucins in the chalaza were the secretives from the basal cells of the magnum epithelium at stage 2 and 3, where egg was coated with albumen.
PAS-positive granules of the luminal epithelium in the isthmus are thought to be a cementing substance in the formation of shell membrane. PAS-positive large granules from the isthmian glands appeared just prior to the egg transportation to the uterus (stage 5) and it served to construct the shell membrane. These large granules contained sulfomucin and sialo- or hyaluromucin, which may be necessary for calcium precipitating sites on the shell membrane.
The basal and the ciliated cells of the uterine epithelia secreted two types of granules. The one contained sialomucin and the other protein richly. They were surmized to be components of the organic matrix in the shell.View full abstractDownload PDF (8976K) -
NOBORU YAMAMOTO, KENJIRO YASUDA1973 Volume 6 Issue 2 Pages 98-106
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe distribution of galactose 1-phosphate uridyltransferase in the bovine liver was investigated by aid of the immunohistochemical method.
The enzyme used in this study was found to contain small amount of contamination by the Ouchterlony gel-diffusion test. The enzyme is present in the liver cells surrounding the central vein. The concentration of the enzyme decreases towards the middle and peripheral regions of a lobule. In the cytoplasm oaf each liver cell, the enzyme is distributed in the coarse granular as well as in the diffuse pattern. In the electron microscopic study the enzyme is recognized in the amorphous part of the cytoplasm, being not associated with any organelles. In the occasional cells, the enzyme is localized only the amorphous part of the cytoplasm. It is never found in the nucleus. Furthermore, the specific staining is not seen in the endothelial cells of the sinusoid, stellate cell of Kuppfer, the cells of the wall of the interlobular bile duct and in the endothelial cells of the interlobular blood vessels.View full abstractDownload PDF (14251K) -
NOBORU YAMAMOTO, KENJIRO YASUDA1973 Volume 6 Issue 2 Pages 107-116
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSAlkaline phosphatase of bovine kidney origin was purified in the homogenous purity and crystallized from the commercial source. Using the purified enzyme, the localization of alkaline phosphatase was examined by immunohistochemical technique and compared with that demonstrated by histochemical method. Only the cells composing the proximal convoluted tubule contained alkaline phosphatase.
On the electron microscopic level, alkaline phosphatase was concentrated in the brush border. The findings were consistent with the distribution pattern of the proper enzyme visualized by the histochemical method.View full abstractDownload PDF (5431K) -
SHUNTA HIROSE, MASAYUKI YASUTOMI, NOBUHIRO MURAI, ZENJI IWASA, MASAAKI ...1973 Volume 6 Issue 2 Pages 117-123
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSWe had previously established a new application method of Bial's reaction to histological sections. In the present study, this new staining method is applied to the human stomach. The results showed a considerably difference from those after PAS reaction, alcian blue staining or colloidal iron reaction. In the normal gastric tissue, Bial's reaction occured more or less definitely in any part of mucosa, muscularis, serosa, connective tissue etc. The strongest reaction was noticed in the chief cells of fundic gland whereas the reaction of the accessory cells and parietal cells was extremely weak. The immature regenerative glands at the peripheral part of ulcer indicated a fairly strong reaction. As for the intestinal metaplasia, the part showing remarkable proliferation also indicated a farily strong reaction, but the reaction of goblet cell mucus remained negative. The reaction of gastric cancerous tissue was rather weak than that of muscularis mucosae or fundic gland irrespective of the type of cancer, e.g., simple cancer, adenocarcinoma or signet cell cancer. The vacuoles of signet cell did not show any positive reaction whereas a farily strong reaction was observed in the exsudate or necrotic substance found in the lumen of well-differentiated papillary adenocarcinoma or tubular adenocarcinoma. The cancer cells infiltrating into the depth of the gastric wall or into the blood vessel, showed a fairly strong reaction.View full abstractDownload PDF (8132K) -
TORU KAMEYA, KEIICHI WATANABE, TAKAYOSHI KOBAYASHI, TATSU MUKOJIMA1973 Volume 6 Issue 2 Pages 124-136
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe localization of human placental alkaline phosphatase (HPALP) was demonstrated by both conventional enzyme-histochemistry and immuno-histochemistry of the indirect peroxidase-labeled antibody method at light and electron microscopic levels. The location of the HPALP was exclusively confined to the microvilli of syncytiotrophoblastic cells by both methods. In early gestational ages, the distribution of the enzyme reaction positive villi was not uniform. At full term, all the syncytiotrophoblastic cells of chorionic villi uniformly showed the most intense staining for the alkaline phosphatase. On tissue sections of the chorionic villi, the HPALP was distinguished from the isoenzymes of small intestine and kidney by the bio-, physico- and immunochemical properties, e.g.: the sensitivity to the stereospecific inhibitor, the L-phenylalanine (0.05M), heat stability (65°C), and the specific reactivity to the anti-HPALP. The specificity and validity of the immunohistochemical staining at the surfaces of the chorionic villi, especially at an ultrastructural level, are throughly discussed. It is suggestive of the usefulness of this im-munohistochemical staining method for the investigation of Megan type isoenzyme produced in certain malignant tumors.View full abstractDownload PDF (15425K) -
M. V. MOJAMDAR, K. S. SHARMA, N. J. CHINOY, V. C. SHAH1973 Volume 6 Issue 2 Pages 137-143
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSBiochemical and histochemical studies were conducted on three different skin regions-black, brown and white-of the guinea pig, with special reference to melanogenesis. Ascorbic acid, ascorbic acid free radical forming special peroxidase and net bound ascorbic acid were found to be more in the brown skin followed by the black and white skins respectively. The brown skin region was also rich in succinic dehydrogenase, ascorbigen and tyrosine than the white and black skin regions. The capacity to utilize ascorbic acid was high in the black skin region, whereas, the white skin showed the highest concentration of proteins.
The histochemical study revealed a greater activity of succinic dehydrogenase, ascorbic acid free radical forming special peroxidase and ascorbic acid in the actively growing melanin synthesizing hair follicles, whereas, the developing hair follicles in the white region stained more intensely for proteins.View full abstractDownload PDF (7299K) -
K. S. SHARMA, M. V. MOJAMDAR, N. J. CHINOY, V. C. SHAH1973 Volume 6 Issue 2 Pages 144-147
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSSulfhydryl groups (-SH) are one of the most potent inhibitors of melanogenesis (Flesch, 1949; Flesch and Goldstone, 1950), and are believed to be one of the causes leading to leucoderma in human beings (Lerner and Fitzpatrick, 1950). Histochemical and biochemical studies of -SH groups were therefore carried out in black, brown and white skin regions of the guinea pig and the skins of the albino and black rats. In general, the albino rat skin showed the highest concentration of -SH groups followed by the white skin region of the guinea pig. The black skin region of the guinea pig and the black rat skin had moderate amounts of -SH groups, whereas the brown skin region of the guinea pig showed the lowest concentrations.
Histochemical localization of -SH revealed intense staining in the hair bulb of the white rat and the white skin region of the guinea pig. The black rat skin as well as the other skin areas of the guinea pig showed lesser staining of -SH groups. The importance of -SH groups in melanogenesis and keratinization are discussed.View full abstractDownload PDF (3180K)
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