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Volume 6 (1973)
- Issue 4 Pages 233-
- Issue 3 Pages 151-
- Issue 2 Pages 89-
- Issue 1 Pages 1-
Volume 6, Issue 3
Displaying 1-7 of 7 articles from this issue
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SAMUEL H. HORI, SATOSHI YONEZAWA1973 Volume 6 Issue 3 Pages 151-162
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe specificity of the lead method for phosphorylase was investigated in rat liver by gel electrophoresis and biochemical and histochemical assays in comparison with the iodine method.
It was found that in the case of the iodine method, inhibition of amylase by ethylenediamine tetraacetate was necessary for a full demonstration of the phosphorylase activity, while in the lead method such interference of the phosphorylase staining by amylase was not a serious problem.
Phosphorylase was markedly inhibited by lead ions and glutaraldehyde, but quantitative and histochemical data indicated that glycogen synthesis could occur even in the presence of lead ions in frozen-substituted sections before and after fixation in glutaraldehyde.View full abstractDownload PDF (5063K) -
HIDEAKI YAMASHITA, HIROHIKO MIKI, ISAMU TSUKAHARA, KAZUO OGAWA1973 Volume 6 Issue 3 Pages 163-170
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe existence and location of the blood-optic barrier of the albino mice eyes was studied by tracing the horseradish peroxidase diffusion which was given into the vein or lateral ventricle of the brain. Following intravenous injection the tracer did not penetrate the capillary wall of the postlaminar portion of the optic nerve while the tracer appeared in the prelaminar portion of the optic nerve (optic nerve head) by diffusion from the surrounding tissue of the posterior scleral foramen. Kuhnt's glial tissue was responsible for the barrier among the prelaminar optic nerve and adjacent retina and the retina was not stained by the horseradish peroxidase reactive products. The tracer which was given into the lateral ventricle appeared in the subarachnoidal space around the optic nerve and peroxidase diffusion from the subarachnoidal space into the adjacent optic nerve was observed.View full abstractDownload PDF (10528K) -
SATORU YOSHIDA1973 Volume 6 Issue 3 Pages 171-182
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe two-wave-length-scanning method in Feulgen cytophotometry was used to study gliogenesis in the human embryos and human fetuses to which 3H-thymidine autoradiography cannot be applied. Various amount of nonspecific light loss was contained in the tissue section of the nervous system. Elimination of non-specific light loss by the two-wave-length-scanning method enabled us to perform accurate measurement of DNA content in an individual nucleus. By this method, proliferating glioblasts could be recognized as cells containing more than diploid DNA amount. As a result, it was concluded that gliogenesis started in the spinal cord between 7th and 8th week in gestational age, and in the cerebral hemispheres between 12th and 20th week in gestational age.View full abstractDownload PDF (7210K) -
MOTOHIKO ITO, TAKAYUKI HARADA, MITSUO BABA, SHIGERU MORIKAWA1973 Volume 6 Issue 3 Pages 183-192
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe distribution of lactate dehydrogenase (LDH) subunits was investigated in normal bovine tissues such as heart, kidney, liver, and skeletal muscle using fluorescent antibody technique. Fluorescence specific for LDH-H was located in the heart muscle fibers, in the cytoplasm and membrane of hepatic cells, and in the cytoplasm of epithelia of distal tubuli. Specific fluorescence of LDH-M was observed in the skeletal muscle fibers and in the cytoplasm of hepatic cells. Immunofluorescence was in accord with the results of histochemical localization by nitro blue-tetrazolium stain. Cold acetone was the best fixative among those examined. But it failed to locate LDH-M in renal tissue, and this observation was discussed.View full abstractDownload PDF (9523K) -
KEI-ICHI HIRAI, KAZUO OGAWA1973 Volume 6 Issue 3 Pages 193-204
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSAn enlargement as well as a remarkable increase in number of peroxisomes was electron microscopically encountered in mouse hepatocytes treated for 7 days with 1, 3-propyl-bis (2-p-chlorophenoxy-2-methylpropanoate) (simfibrate, BCPMP), a hypolipidemic drug, that lowers serum chloesterol and triglycerids. Increase in number of peroxisomes was approximately 3 times of that in control cells. The general size of enlarged peroxisomes was 1.33×1.07μ, and approximately 2.5 times as compared with normal peroxisomes, 0.54×0.46μ, in control hepatocytes.
The use of the term “giant peroxisomes (megaloperoxisomes)” is proposed in the present investigation.
Giant peroxisomes had protein-riched matrix surrounded by a single membrane, and many of them contained a single nucleoid. Continuities or close associations between giant peroxisomes and the smooth or rough endoplasmic reticulum were frequently observed. Further detailed investigations on the origin of giant peroxisomes and relationship to pharmacological effect of BCPMP will be achieved.View full abstractDownload PDF (16801K) -
SETSUYA FUJITA, TSUKASA ASHIHARA, MASARU FUKUDA, TAKANORI HATTORI, SAT ...1973 Volume 6 Issue 3 Pages 205-211
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA new method is described to measure DNA contents of labeled and unlabeled cells in autoradiographs without removing silver grains. Smears of regenerating rat liver, flash-labeled with 3H-thymidine, were made on cover glasses, stained by Feulgen method and processed for autroadiography. The cover glass was mounted on a slide glass, with the emulsion side facing downward, so that Feulgen cytofluorometry with incident light excitation can be carried out without interference by silver grains in the emulsion. Transmitted light illumination which can be turned on at any time allowed observation of cell image and silver grains under the same cell. Cytofluorometric data obtained by this method proved that this is a simple, rapid and reliable method of determining DNA contents on labeled and unlabeled cells in autoradiographs. Possibility is discussed to apply this method generally for determining amount of fluorescent substances in combination with autoradiography.View full abstractDownload PDF (2611K) -
TAKUMA SAITO, KAZUKO IWATA, KAZUO OGAWA1973 Volume 6 Issue 3 Pages 212-229
Published: 1973
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSChanges of peroxisomes in the rat hepatic parenchymal cells during the regeneration following partial hepatectomy were investigated ultracyto-chemically.
For the demonstration of peroxidatic activity of catalase frozen and nonfrozen sections from tissues fixed in 2% glutaraldehyde for 2 hours at 0°C or 20°C were incubated in Novikoff-Goldfischer medium (1969) mainly at pH 9.0 for 1 to 2 hours at 37°C. After incubation, they were post-osmificated and processed for electron microscopic observation.
The peroxidase activity was demonstrated in peroxisomes (0.2-0.7μ) and occasionally in outer and intracristal spaces of mitochondria in the normal and regenerating hepatic parenchymal cells. The smaller peroxisomes (0.1-0.2μ) having weaker activity may be the developing peroxisomes. In Kupffer cells, cisternae of the endoplasmic reticulum showed the positive enzymatic activity.
The number of peroxisomes per cell calculated on the toluidine blue-stained epon sections (1μ thick) showed decrease till 24 hours following hepatectomy. However, the number of peroxisomes revealed gradual recovery at 28-30 hours. Marked decrease of the peroxisome number in hepatic parenchymal cells was noticed in the normal rat, which received a single intraperitoneal injection of Actinomycin D (25μg/100g body weight).View full abstractDownload PDF (28039K)
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