All issues
Successor
Volume 13 (1967)
- Issue 6 Pages 303-
- Issue 5 Pages 239-
- Issue 4 Pages 183-
- Issue 3 Pages 121-
- Issue 2 Pages 65-
- Issue 1 Pages 1-
Volume 13, Issue 6
Displaying 1-9 of 9 articles from this issue
- |<
- <
- 1
- >
- >|
-
[in Japanese]1967 Volume 13 Issue 6 Pages 303-310
Published: December 30, 1967
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSDownload PDF (938K) -
Mamoru Sugiura, Manzo Ito, Kazutada Okumura, Ryoichi Hatano1967 Volume 13 Issue 6 Pages 311-318
Published: December 30, 1967
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSA new method for the separative determination of filamentous fungi amylase was described. The procedure was as follows : 5ml of 1.0% soluble starch solution, 3 ml of M/10-acetate buffer solution (pH 5.0) and 1 ml of enzyme solution in 50 ml Elrenmyer flask, was incubated at 37°C for 30 minutes. After addition of 1 ml of 1 N-NaOH solution to stop the reaction, dextrose formed in the reaction mixture was determined by the Tauber-Kleiner's method (glucoamylase activity). By using the Fehling-Lehmann-Schoolr's method in stead of the Tauber-Kleiner's method, free reducing sugar in the reaction mixture was determined (total amylase activity). Glucoamylase activity subtracted from total amylase activity was defined α-amylase activity. One unit of amylase activity was defined as the amount of enzyme which produces 100 μM of glucose from starch per one hour at 37°C.View full abstractDownload PDF (854K) -
Mamoru Sugiura, Manzo Ito, Hidero Tanaka1967 Volume 13 Issue 6 Pages 319-322
Published: December 30, 1967
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSA new method for the separative determination of filamentous fungi amylase was described. The method is based on the measurement of the difference of calibration curve of amylase. The assay procedure was as follows : 1 ml of a enzyme solution diluted properly was incubated for 30 minutes at 37°C with 9 ml of the substrate solution (5 ml of 1% soluble starch solution and 4 ml of M/10-acetate buffer solution, pH 5.0). The enzyme reaction was interrupted by the addition of 1 ml of 1N-sodium hydroxide solution. After addition of 10 ml of Fehling's reagent, this reaction mixture was boiled for 2 minutes on direct fire, and cooled then to the reaction mixture 5 ml each of potassium iodide solution (30%) and diluted sulfuric acid (25%) was added. Iodine liberated hereby was titrated with N/20 sodium thiosulfate. (A) By using 6 times diluted enzyme solution of the previous enzyme solution, free reducing sugar was determined by the same manner (B). Unknown samples are then determined from coefficient table, which is prepared from experiments with the standard sample.View full abstractDownload PDF (552K) -
Kikuhiko Okamoto, Tomoo Ito, Akira Hasegawa, Goro Urakubo1967 Volume 13 Issue 6 Pages 323-329
Published: December 30, 1967
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSDistribution after peroral injection, percutaneous absorption and residual amount on skin surface of zinc bis (2-pyridylthio)-1, 1'-dioxide (Zinc Omadine) labelled with 35S and 65Zn were observed. Following the peroral injection, this compound was decomposed in stomach. 65Zn-compound was excreted mainly in feces and 35S compound in urine. Percutaneous absorption of Zinc Omadine following the application of shampoo, ointment and water suspension containing the compound to skin was very slow and little. Residual amount on skin surface after application of the preparations was less than 1% of the amount of Zinc Omadine applied.View full abstractDownload PDF (674K) -
Youki Ose, Hirokatsu Akagi, Shouzo Morishita, Hayaki Niwa, Yoshimi Fur ...1967 Volume 13 Issue 6 Pages 330-334
Published: December 30, 1967
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSSanitation of swimming pool by using tablets of neutral Ca (CIO)2 salt was studied. This tablet has 20 g weight, 30 mm diameter and about 16 mm height. The dissolving speed of tablet in pool water will be shown by next formula. [numerical formula] It was found that the theoretical values from the formula (3) and (3') were accorded experimental values. Residual-chlorine concentration will be calculated by next formula. The experimental value was accorded with the value obtained from the formula. [numerical formula] This tablets dissolved throughly in 2.5 hours, but some of them were disappeared in short time by dissolving of getting out of its shape. Some tablets were moved to other place at the bed of pool, but the concentration of residual-chlorine was held uniformly.View full abstractDownload PDF (566K) -
Senji Suenaga, Suzuko Aoki1967 Volume 13 Issue 6 Pages 335-341
Published: December 30, 1967
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSIn the examination of water, the standard of turbidity is the kaolin suspension of 1 g/L in Japan officially. In this method, the coefficient of variation is about 28% in intensity of turbidity of standard suspension prepared from various kaolin articles of commerce, and is about 8%, prepared from purified kaolin obtained through the sedimentation method (reference to report I). In the method that kaolin suspension is standardized by transmissible light path length in the standard turbidimeter based on"the standard methods for the examination of water and waste water of APHA" the coefficient of variation is about 3.9% in intensity of turbidity of standard suspension standardized. This standard turbidimeter is more convenient for routine works than the candle turbidimeter of"the standard methods of APHA" In stead of the standard turbidimeter, the transmittance grademeter with double cross mark is available as simple method, but in this case the coefficient of variation is about 8%. In the examination of transmittance grade of water by this transmittance grademeter with double cross mark, precision is higher than by the Japan water works association method with No. 5 type character mark.View full abstractDownload PDF (817K) -
Teruo Fukuda, Kimie Miyakawa, Tamiko Ueki1967 Volume 13 Issue 6 Pages 342-345
Published: December 30, 1967
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSBy the combination of the method for isolation and concentration by using NH4SH and the calculation RMo at the paper electrophoresis, following injurious nine metals were separated and identified : Hg, Pb, As, Sb, Cu, Zu, Ag, Cd and Bi. Three test solutions for the paper electrophoresis were prepared by the NH4SH method. Cu, Zn, Ag, Cd and Pb, belong to the 1st section, were separated and identified according to RMo by using 1N citric acid, 5N acetic acid and 5N formic acid as the electrolyte for the paper electrophoresis. Hg and Bi, the 2nd section, were separated and identified as stated above by using 0.1M lactic acid or 5N formic acid as the electrolyte. As and Sb, the 3rd section, were separated and identified as stated above by using 0.1M lactic acid as the electrolyte.View full abstractDownload PDF (571K) -
[in Japanese]1967 Volume 13 Issue 6 Pages 348-351
Published: December 30, 1967
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSDownload PDF (585K) -
[in Japanese]1967 Volume 13 Issue 6 Pages 351-352
Published: December 30, 1967
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSDownload PDF (411K)
- |<
- <
- 1
- >
- >|