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Volume 21 (1975)
- Issue 6 Pages 301-
- Issue 5 Pages 239-
- Issue 4 Pages 169-
- Issue 3 Pages 117-
- Issue 2 Pages 51-
- Issue 1 Pages 1-
Volume 21, Issue 5
Displaying 1-10 of 10 articles from this issue
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[in Japanese]1975 Volume 21 Issue 5 Pages 239-255
Published: October 31, 1975
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSA review has been presented on the basic principle and characteristics of radioimmunoassay, methods for preparation of immunogens and labeled haptens, immunization techniques, and general procedure and specificity of radioimmunoassay. Homogeneous immunoassay, spin and enzyme immunoassay, has also been stated briefly. The examples cited, morphine, lyergic acid diethylamid (LSD), 2, 5-dimethoxy-4-methylamphetamine (DOM), mescaline, barbiturates, tubocurarine, diazepam, nicotine, amphetamine, fentanyl, and diphenylhydantoin, are almost restricted to drugs relating to forensic chemistry, on which radioimmunoassay has developed to date. The sensitivity and specificity under the conditions used for radioimmunoassay are given for each sample. The drugs described above have been determined rapidly and specifically, in the order of nanogram to picogram in body fluids without pretreatment of samples. Finally, it is concluded that the sensitive, specific, and rapid radioimmunoassay will take the place of other analytical techniques for the determination of effective drugs in human body in future.View full abstractDownload PDF (1597K) -
HIROSHI OBA, TAKEO YAMAMOTO, YOKO YOSHIKAWA, KISAKO IMAMOTO, LEIKO YAM ...1975 Volume 21 Issue 5 Pages 256-260
Published: October 31, 1975
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSSulfur content in orange leaves was determined by microbiological assay method using Escherichia coli B (H), and concentration of sulfur oxides in air was discussed. Sulfur content in each orange leaf of one grown-up tree gave large standard deviation (11-29%), and no significant correlation was observed between this sulfur content and concentration of sulfur oxides in air. On the other hand, some significant correlation was observed between sulfur content in orange leaves of young trees grown in a pot (pot-test) and concentration of sulfur oxides in air. These result suggested that the sulfur content in orange leaves obtained by this pot-test method was useful as an index of concentration of sulfur oxides in air.View full abstractDownload PDF (569K) -
ISAMU NAGAI, HIDEO OKA, MIWAKO TASAKA, ARATA OKA1975 Volume 21 Issue 5 Pages 261-266
Published: October 31, 1975
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSA new methylation method for gas chromatographic determination of saccharin in foods was established. Saccharin was extracted from samples with ethyl acetate and converted to N-methylsaccharin by reaction with dimethyl sulfate in acetone at room temperature for 10 min. After decomposition of dimethyl sulfate with sodium hydroxide, N-methylsaccharin in the solution was determined by FID-gaschromatography using a column of SE-30 on Chromosorb W. Methyl palmitate was used as an internal standard. Rate of methylsaccharin formation from saccharin was 90.6-96.6%. This gas chromatographic method gave good results when applied to the determination of saccharin in several kinds of food and the recovery of saccharin added to food at the levels of 150 and 240 ppm was 77.3-94.3%.View full abstractDownload PDF (675K) -
AKIO KAMIYA1975 Volume 21 Issue 5 Pages 267-270
Published: October 31, 1975
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSA method for determination of vanadium in air by flameless atomic absorption spectrophotometry was studied. Atomospheric particulates were collected on a Gelman filter by a high-volume air sampler, and extracted with 5% hydrochloric acid. Cupferron, a chelating agent, reacts with vanadium to form a complex, which can be extracted with methyl isobutyl ketone. The solvent was dried, ashed, and atomized by passing through a high electric current of the atomizer. The concentration of vanadium was calculated directly from the calibration curve by measuring the absorbance of vanadium at 318.5 nm. Cations, except tin, did not interfere the determination of vanadium seriously. Analytical results were in good agreement with those obtained by a nitrous oxide-acetylene flame atomic absorption spectrophotometry. The coefficient of variation was from 4.4% to 9.4%, and the limit of detection was 0.03 ppm of vanadium, when the signal/noise ratio was 2.View full abstractDownload PDF (519K) -
AI NAKANISHI1975 Volume 21 Issue 5 Pages 271-274
Published: October 31, 1975
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSBased on the fact that the Schiff base, of p-dimethylaminobenzaldehyde and aromatic primary amines emit fluorescence in aqueous or ethanolic solution containing hydrochloric acid, quantitative determination of m-toluidine and sulfamine was made by using a longer wave length in fluorescence spectra of the Schiff base in ethanolic solution containing hydrochloric acid.View full abstractDownload PDF (498K) -
TSUYOSHI MURAKAMI, AKIKO KIDA, MIYOKO NAKAI, SHOJI MATSUNAGA1975 Volume 21 Issue 5 Pages 275-281
Published: October 31, 1975
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThis investigation was made to obtain the control values for estimation of the existing state of sea sediments in Hiroshima Prefectural area contaminated with heavy metals in the Inland Sea. 1. After determination of the concentration of heavy metal elements in 50 samples of sediments obtained from environments scarcely affected by direct industrial influence, correlations between sediment samples were examined by calculating the ratio of elements, and then samples more correlated with others were selected as a representative group. The mean values of heavy metal element concentration in the representative group, were used as control values. 2. It was interesting that the heavy metal elements in the representative group can be divided into two groups by the tendency of their correlations. One group includes Hg, Cd, Pb, and Cu, and the other, Fe, Ni, Zn, Mn, and Cr. 3. It seems that this method is applicable for wider range of samples such as water or biological meterials other than sediments. For these purposes inorganic and organic materials can be selected, as well as heavy metals.View full abstractDownload PDF (783K) -
MASAYOSHI YAMAGUCHI, TAKEO YAMAMOTO1975 Volume 21 Issue 5 Pages 282-285
Published: October 31, 1975
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThis experiment was made to examine whether the inhibitory effect of thyrocalcitonin (TCT) on calcium efflux is exhibited in liver slices from the lead-treated rats. Lead acetate solution of 20 mg Pb/100 g body weight was intraperitoneally administered. After 36 hr, animals were killed by decapitation. The liver slices were preincubated in 2 ml of krebs-Ringer bicarbonate buffer (pH 7.4) containing 2.5 μCi 45Ca with 95% O2-5% CO2 as the gas phase. After preincubation, the slices were transferred to unlabeled media with or without TCT (8.0 MRC mU/ml), and incubated for an additional 30 or 60 min. Radioactivity was counted on a scintillation spectrometer. In the liver slices from normal ral, the rate of calcium efflux is inhibited in the presence of TCT, and the decrease of calcium efflux rate after TCT treatment was about 25% of the control. On the other hand, in the liver slices from the lead-treated rat, the calcium efflux rate was inhibited by TCT treatment. Approximately 30 or 50% of control decreased during the incubation period of 30 or 60 min, respectively. The present results suggest that liver calcium accumulation induced by lead administration is caused by the inhibitory effect of TCT on the calcium efflux in liver cells.View full abstractDownload PDF (557K) -
TOMOHIRO YAMAGUCHI, HIROYASU KANESHIMA, TAKASHI YAMAGISHI1975 Volume 21 Issue 5 Pages 286-288
Published: October 31, 1975
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSAttempt was made to find a rapid and sensitive method for detecting some psychotropic drugs by thin-layer chromatography, using a new apparatus equipped with a flame ionization detector. By using this method, added drugs were successfully separated and identified from the urine. This method was considered to be useful for the detection of psychotropic drugs in urine.View full abstractDownload PDF (365K) -
TAKEO YAMAMOTO, MASAYOSHI YAMAGUCHI, HIDEJI SATO1975 Volume 21 Issue 5 Pages 289-293
Published: October 31, 1975
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThe activity of β-glucuronidase and β-galactosidase in the femur of rats was examined after single intraperitoneal administration of lead (10.0 mg/100 g) or cadmium (0.15 mg/100 g) acetate, and the following results were obtained. Bone β-glucuronidase and β-galactosidase activities increased significantly (p<0.01) 1 day after lead or cadmium administration compared with control rats, and these enzyme activities were significantly increased 7 days after lead or cadmium administration. Bone β-glucuronidase and β-galactosidase activities were slightly elevated by the administration of a mixture of lead and cadmium. These results suggest that lead or cadmium may cause metabolic disorder in the bone tissue of rats.View full abstractDownload PDF (610K) -
AKIRA OGAMO, NORIKO WAKAIZUMI, KINZO NAGASAWA1975 Volume 21 Issue 5 Pages 294-299
Published: October 31, 1975
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSA clean-up method suitable for the detection of carbamate insecticides in unpolished rice, green tea, cabbage, and spinach by thin-layer chromatography was developed. The clean-up method consists of the treatment of food extracts with a coagulating solution, followed by washing with an alkaline solution. Washing with sodium benzoate solution is effective for removing most of caffeine in the extract of green tea. Washing with an acidic solution is necessary for the removal of contaminants in the extracts of cabbage and spinach. By this method, carbaryl (0.1 ppm) added to the food was finally recovered in an ethanolic solution (100 μl), and the detection limit on thin-layer plates was 4-5 μl. The detection of Suncide, Meobal, and Osbac from the food fortified at 1 ppm each was successful by the application of 3-7 μl of the finally obtained solution to thin-layer chromatography.View full abstractDownload PDF (681K)
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