-
[in Japanese]
1964 Volume 10 Issue 1 Pages
1-5
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
-
Tetsukichi Niwaguchi
1964 Volume 10 Issue 1 Pages
6-8
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
Separation and identification of several kinds of barbituric acid derivatives by thin-layer chromatography were investigated. The thin-layer was made of a mixture of silicic acid, potato starch and sodium carbonate. The most suitable solvent system among those tested was ethylene dichloride-acetone (85 : 15). Spectroscopic characterization of the compounds submitted to the chromatography was performed by eluting the spots with 0.5 N ethanolic sodium hydroxide.
View full abstract
-
Seiichi Okui, Yasuo Suzuki, Hisako Takeda
1964 Volume 10 Issue 1 Pages
9-13
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
o-Nitrophenyl butyrate was used as a substrate for measuring blood esterase activity. Human serum esterase was inhibited almost completely by 10
-6 M of TEPP, 10
-7 M of Phosdrin and 10
-4 M of Eserin. Mouse serum esterase was inhibited to the extent of 80 and 98 % in the same concentration of Eserin and TEPP respectively. On the other hand, inhibition of rat and rabbit esterases by above inhibitors are markedly slight, particulary that of rabbit is inhibited only less than 5 % by Eserin treatment. This is probably caused by high content of A-esterase in the serum. It was recognized that human and pig serum are most suitable as the enzyme sources for the analysis of organophosphorous insecticides remaining in plants by the choline esterase inhibition method.
View full abstract
-
Akira Hamada, Otomatsu Hoshino, Keiko Tanzawa, Tyunosin Ukita
1964 Volume 10 Issue 1 Pages
14-17
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
The gas chromatographic analyses of 11 local anesthetics, meprylcaine, hexylcaine, anesthesin, procaine, tutocaine, cornecaine, tetracaine, lidocaine, carbocaine, dibucaine, and cocaine, in the form of free bases, are described. Microgram samples, in ethanol solvent, are subjected to separation in the argon gas chromatography with a column containing 1 % SE-30, 1 % Fs-1265 or Thermol-Q as the stationary phase. The best separation was generally obtained by using the column of Thermol-Q.
View full abstract
-
Tatsuo Ozawa, Satoshi Nagaoka, Katsuaki Sumino
1964 Volume 10 Issue 1 Pages
17-21
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
Micro-diffusion analysis coupled with colorimetry has been used as a convenient method for determination of ethanol. In the present experiment the optical density of the green color of Cr
3+ formed by oxidation of ethanol with K
2Cr
2O
7-H
2SO
4 was measured at 610 mμ. Half milliliter of ethanol (0.1∼0.5% (v/v)) and 1 ml. of K
2Cr
2O
7 solution (1.0 g. of K
2Cr
2O
7 was dissolved in18.5 N H
2SO
4) were used.
View full abstract
-
Saburo Kanno, Shogo Ochiai, Takashi Tanabe
1964 Volume 10 Issue 1 Pages
22-26
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
A sample of well water in Yokohama city was found to contain both the residual chlorine and nitrite nitrogen. Ammonia nitrogen (7.5 ppm) too was found in this water. According to the conventional concepts, it seemed impossible that the residual chlorine and nitrite nitrogen could exist in the water at the same time. We found the nitrite nitrogen could be produced under certain conditions from ammonia nitrogen in the presence of residual chlorine. Thus, when water, the pH of which was above 8.0 and which contained several ppm of ammonia nitrogen, was disinfected with chlorine, the production of nitrite nitrogen was detected. However, when the pH was lower than 7.0 or the amount of ammonia nitrogen was less than 1 ppm, the nitrite nitrogen could not be detected.
View full abstract
-
Tetsuo Inoue, Taro Kawamura, Masaya Kamijo, Michiko Asakura
1964 Volume 10 Issue 1 Pages
26-31
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
The distillation method for determination of sorbic acid in some food especially in boiled beans is disturbed by other U. V. absorbing substances probably derived from sugar by heating. Although the distillate must be collected as much as possible in order to obtain sorbic acid in good yield, the contaminating substances also increase in this procedure. These contaminants were found removable by treating with ether and 0.002 N NaOH solution.
View full abstract
-
Hidemi Hayashi
1964 Volume 10 Issue 1 Pages
32-36
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
Ammonia and albuminoid-N as contaminants of public bath water, could be separately estimated by the following simple procedures. Sample to be analyzed was diluted with equal volume of distilled water. Two ml of the diluted sample solution was added with 0.1 N Na
2HPO
4 to make the solution slightly alkaline (pH 8.5) and mixed with 1 ml of 0.1 per cent solution of trinitrobenzene sulfonic acid (TNBS). The mixture was kept in a dark place at 40° for 2 hours, and the resulting orange-colored solution was extracted with ethyl acetate three times. The volume of combined ethyl acetate-extractes was adjusted to 5 ml and the optical density at 320 mμ was measured against ethyl acetate-extract of the blank solution obtained by treating 20 ml of distilled water in the same procedure. Ammonia-N could be estimated from the reading and standard curve shown in Fig. 3. The remaining aqueous solution was acidified by adding 1.0 ml of N-HCl and the volume was adjusted to be 5.0 ml (or more if necessary). From the optical density against blank solution, albuminoid-N could be estimated with the use of a standard curve obtained on glycine (Fig. 2). The contamination of public bath water proceeded as shown in Fig. 6 and the amounts of albuminoid-N were always about twice as much as ammonia-N.
View full abstract
-
Isawo Saito, Yasuyoshi Sayato, Michiko Nakata, Haruyo Tomobe
1964 Volume 10 Issue 1 Pages
37-41
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
Nash reported that HCHO reacted with acetylacetone in the presence of excess ammonia to produce 3, 5-diacetyl-1, 4-dihydrolutidine (DDL). The joint commitee of Association of British Chemical Manufactures and Society for Analytical Chemistry proposed DDL-colorimetry as a standard for detection of HCHO in effluent water. The authors investigated further on DDL-colorimetry and obtained the following results. After 20 min. at 60° and pH 6.5 the coloration of DDL formed was maximum. The color of DDL-solution was stable for 72 hr. in a sealed container and able to be determined spectrophotometrically at 425 mμ when 1∼8 ppm. of HCHO was present. Coexistence of H
2S with HCHO in effluent water inhibited the formation of DDL, but the addition of zinc acetate solution at the time of distillation could remove this disturbance. By distillation repeated three times with addition of water 100 % of HCHO in effluent water was recoverable.
View full abstract
-
Sadao Iguchi, Magobei Yamamoto, Toshinobu Aoyama
1964 Volume 10 Issue 1 Pages
42-46
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
Analysis of food preservatives by gas chromatography was studied. A mixture of sorbic acid, benzoic acid, salicylic acid, dehydroacetic acid, 2-methyl-1, 4-naphthoquinone, and methyl, ethyl, propyl and butyl p-hydroxybenzoate was completely separated without any prior treatment under the conditions of DC 550 silicone on Celite 545, at 190°, with carrier gas of hydrogen. Dehydroacetic acid in butter, and sorbic acid and alkyl p-hydroxybenzoate in sauce "SHOYU" were extracted with ether from the aqueous acidic solution and readily identified. Using 3, 4-dimethylphenol for sorbic acid and dehydroacetic acid, 2-methyl-1, 4-naphthoquinone for alkyl p-hydroxybenzoate as an internal standard, calibration curves for the assay were obtained. Dehydroacetic acid in butter was determined with the mean recovery of 99 % and with the standard deviation of 2.9 %. Procedures for preparation of sample are presented in detail.
View full abstract
-
Tetsuo Inoue, Taro Kawamura, Masaya Kamijo, Michiko Asakura
1964 Volume 10 Issue 1 Pages
47-51
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
The experimental conditions applied to the separation of sorbic acid in foods by dialysis, that is, the effects of membrane, solvent, temperature and some salts added were studied. Moreover, the methods to remove the substances disturbing the measurement of U. V. spectrum and to increase the recovery of sorbic acid were investigated. Ultraviolet absorption spectra of dialyzed solution was measured without further treatment as a preliminary test and after removal of inhibitors as a plenary test. Thus, the detection and the determination of sorbic acid were found to be possible. This method was applied to the 336 kinds of market foods and the results obtained were reported.
View full abstract
-
Noboru Suzuki
1964 Volume 10 Issue 1 Pages
52-57
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
The author calculated the mean and standard deviation of 35 estimated values of water gathered from many rivers, and compared them with those of well water. The correlation coefficient among 22 estimated values obtained from river water was calculated by using 178 sheets of correlation charts. The equation of regression line were presented for the strongly correlated groups, by use of which one can estimate a value from another strongly correlated one. The picture of regression lines were also given. About BOD, multiple correlation and regression plane were analysed.
View full abstract
-
Shigeharu Ichikawa, Minoru Nanjo, Shizuo Kano
1964 Volume 10 Issue 1 Pages
58-60
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
Sodium lauryl sulfate (SDS) or sodium N-lauryl sarcosinate (SLS) is contained in toothpaste as forming agent and surfactant. Besides these agents sodium alkyl benzensulfonate (ABS) is suspected to be used for similar purpose. In order to ascertain this suspicion some commercial tooth-pastes (including foreign articles) were tested. In the case of the sample which contained SDS and ABS in the ratio of seven to three ABS was detectable by infrared absorption spectrum as a method for detection. (With this method the presence of SLS had no effect on detection of ABS) Five micrograms of ABS in the mixture composed of 200 mg of SDS and 5 mg of ABS was detectable by paper chromatography of the hydrolysate using pinakryptol yellow as a coloring agent. The surfactants were extructed from the commercial tooth-pastes and tested in this way, but ABS was not detected.
View full abstract
-
Kazuo Sato
1964 Volume 10 Issue 1 Pages
61-64
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
In Spite of the recent development in making less poisonous pesticides, there have been still a large number of accidents caused by the pesticides. We performed the determination of activity of acetyl choline esterase in blood of ca. 10, 000 persons who were handling organophosphorous pesticides. The Ishido-(Keio University)-method was employed for the determination. The results could be summerized as follow. (1) Poisonous : 3.62%, Critical, in spite of lacking symptoms : 17.82%, Normal : 78.56%. (2) The activity of the esterase was apt to be lowered more easily in female than in male. (3) The lowering was more evident in older persons. (4) The lowering was intense in wet season during which there could be many chances to handle pesticides. (5) Some relationships were found between the number of poisoning cases and of yeas of their handling-experiences.
View full abstract
-
Kazuo Morishita, Kiichiro Takahashi
1964 Volume 10 Issue 1 Pages
65-67
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
Influences of radiation of stove on pupils in school room were investigated. The inquiries to the pupils indicated that the distance of pupils nearest to the stove was 1.0∼1.6 meters and the pupils felt uncomfortable and feverish. The mean value of differences (ΔT) between the temperature indicated by Grove thermometer (TG) and the air temperature (TA) was 9.8°C around the stove. By setting a shading board the ΔT could be decreased to 2.4°C and pupils feverish feeling disappeared simultaneously.
View full abstract
-
Tsuguo Kobayashi
1964 Volume 10 Issue 1 Pages
68-71
Published: March 30, 1964
Released on J-STAGE: May 30, 2008
JOURNAL
FREE ACCESS
Decreasing tendency of the activity of palmar disinfectants is important in their practical use. The decrease of disinfecting activity of some antiseptic solutions by standing at room temperature (23∼32°) was examined by the phenol coefficient test using Serratia marcescens. It took 10 days for the phenol coefficients of liquefied phenol and saponated cresol solution. and took more than 20 days for those of benzalkonium chloride and mercuric chloride solutions to decrease to the half of the initial coefficient. Among the activity of these four antiseptic solutions, that of benzalkonium chloride solution was most markedly decreased by hand-washing.
View full abstract
