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Volume 15 (1969)
- Issue 6 Pages 331-
- Issue 5 Pages 269-
- Issue 4 Pages 213-
- Issue 3 Pages 105-
- Issue 2 Pages 41-
- Issue 1 Pages 1-
Volume 15, Issue 4
Displaying 1-10 of 10 articles from this issue
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[in Japanese], [in Japanese]1969 Volume 15 Issue 4 Pages 213-218
Published: August 30, 1969
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSDownload PDF (788K) -
TAKEHIKO MATSUSHITA1969 Volume 15 Issue 4 Pages 219-224
Published: August 30, 1969
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSRecent investigations had been made only on the biological studies of iron bacteria, one of organisms attributable to many evils caused by them with larger use of underground-water. This paper was described the studies on basal media for growth of iron bacteria, and variations of soluble irons (amounts of total iron and ferrous ion) in the media and organic matters in the precipitates. The bacteria (Gallionella ferruginea) was grown at 20±0.5° in the modified media of ones described by Lieske, Wolfe and Mudge, respectively, for which the varied concentration of nutrient salts, iron powder as its source and ventilation of CO2 gas was employed. The bacteria was reproducibly cultivated and its growth was terminated after 7 to 10 days incubation in the modified media.View full abstractDownload PDF (1169K) -
SADAO NIIMI, HIDEKO ISHIHARA, HIROH IKEZAWA, OTOHARU ISHIZAKA1969 Volume 15 Issue 4 Pages 225-231
Published: August 30, 1969
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSIn the process of photosynthesis, carbohydrase was externally secreted when the cells of Chlorella vulgaris were cultivated on Arnon medium. Especially when starch was added to the culture medium, induced enzyme was secreted during the course of cultivation. By its secreted carbohydrase, the multiplication of chlorella was promoted. The culture supernatant of chlorella was lyophilized and used as the source of raw carbohydrase. The carbohydrase activity was measured by the method of blue value and the properties of the enzyme were investigated. The optimal pH was 7.0 and the optimal temperature was 35°. Significant amount of enzyme activity was lost at more than 50° and the action of the substrate-protection was observed. As the effect of metal salts, the activity was increased in the presence of 3.3×10-4M of Ca2+, Mg2+, Fe3+, Mn2+ and Fe2+, and inhibited in 3.3×10-4M of Ag+ and Hg2+. Michaelis constant, Km, was 1.2 mg/ml to amylose as substrate. The effect of carbohydrase on chlorella cultivation was investigated. When cultivation of chlorella was performed in the presence of starch and purified amylase, its yield in dry weight was 3.5 times as much as that of standard. When coexistent bacilli with chlorella was counted on agar plates, its numbers ; bacilli : chlorella cells=1.79 : 108 and those bacilli did not secreted carbohydrase.View full abstractDownload PDF (779K) -
MASAYOSHI NAKAKUMA, SHOZABURO OGIYA1969 Volume 15 Issue 4 Pages 232-237
Published: August 30, 1969
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSTha micro quantitative determination of Ca in serum with glyoxal bis (2-hydroxyanil) (GBHA) was investigeted. Of many metal ions which are present with Ca in serum, Na, Mg and Pb did not form complex with GBHA. Zinc and Cu are masked by adding 10% NaCN solution to the reaction mixture, Fe and Mn are also masked with 10% triethanolamine. n-Butanol was the most preferable solvent for the extraction of the colored chelate component of Ca with GBHA, because of high sensitivity and no interference with yellow colored serum bilirubin. Methanol and sodium hydroxide-sodium borate buffer were used for the solvent for the preparation of the GBHA reagent solution and for color development, respectively. The stability of the colored chelate in the solution was good. The colorimetric determination of Ca in serum gave good agreements in the recovery of added Ca to serum and in comparison with earlier Clark-Collip's method.View full abstractDownload PDF (647K) -
YASUHIRO YAMANE, MOTOICHI MIYAZAKI, TSUYOSHI IMAHASHI, SHOJI BANDO, YU ...1969 Volume 15 Issue 4 Pages 238-243
Published: August 30, 1969
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSMeasurements by radioactivation analysis were carried out for aluminium and manganese in deposits which were collected from various sampling points in Chiba areas. From the analytical data, the seasonal and geometrical fluctuations of the amount of aluminium or manganese in the deposits were also discussed. The deposits were collected monthly for a year, and each 10 mg ash of the deposit was irradiated for 1 min at neutron flux, 3×1010n/cm2·sec, (5 kw) for aluminium, and 8×1013n/cm2·sec, (10 Mw) for manganese, respectively, and their γ ray spectra were analyzed. (aluminium : 1.78 MeV, manganese : 0.83 MeV) The values of aluminium obtained by this method were coincided well to the ones of aluminium reported previously which were determined by the ordinary chemical analysis. Seasonal and geometrical fluctuations were not observed clearly for aluminium contents, however, they were quite definite in the case of manganese, and large amounts of manganese were found in deposits in the industrial divisions of Chiba, especially near to iron works.View full abstractDownload PDF (681K) -
TAKIO NAITO, HIDEO NAGANO, TERUYO YASUI, TOMOKO ISHIHARA1969 Volume 15 Issue 4 Pages 244-247
Published: August 30, 1969
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThe reaction of 3-methyl-2-benzothiazolinone hydrazone (MBTH) with sugars resulted in the development of green to blue coloration, which has an absorption maximum at about 635 mμ. Therefore, the colorimetric determination of sugars using MBTH was examined. To 1 ml of sample, 1 ml of 0.4% MBTH solution and 2 ml of 0.05 N NaOH are added, and the mixture is heated in a boiling water bath for 10 minutes, then cooled under cold tap water to room temperature. Five ml of 1% ferric chloride solution are added. Following about 10 minute waiting period, the mixture is extracted with n-butyl alcohol, then the absorbance at 635 mμ is mesured. The concentration range of glucose determinable by this method is 5-70 μg/ml, and the determined value agree with Somogyi-Nelson's method within the largest deviation of ± 3%.View full abstractDownload PDF (470K) -
NOBORU YAMATE1969 Volume 15 Issue 4 Pages 248-252
Published: August 30, 1969
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThe auto exhaust was irradiated with xenon light or solar light under static conditions, and the concentration changes of hydrocarbons, nitrogen oxides, formaldehyde and irradiation products were analysed. The concentrations of each component were determined by hydrogen flame ionization gas chromatography for hydrocarbons, and colormetry for nitrogen oxides and formaldehyde, and electron capture gas chromatography for irradiation products, respectively. From these experiments, it was noticed that the concentrations of hydrocarbons and nitrogen oxides decreased, whereas that of formaldehyde increased. The irradiation products were also found to be methyl nitrite, ethyl nitrite, methyl nitrate, ethyl nitrate, n-propyl nitrate, isobutyl nitrate, biacetyl and PAN. Therefore these photochemical reactions of auto exhaust were assumed to occur in the atomosphere.View full abstractDownload PDF (587K) -
TOSHIYUKI ITO, HIROH IKEZAWA, OTOHARU ISHIZAKA1969 Volume 15 Issue 4 Pages 253-257
Published: August 30, 1969
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThe effects of food dyes on tryptic hydrolysis of casein were studied in vitro. The assay methods used were as follows : xanthene derivatives (erythrosine, eosine, phloxine, rose bengal) by Kunitz's method, tartrazine by direct Anson's method, and azo derivatives (amaranth, new coccine, ponceau 3R, ponceau SX, sunset yellow FCF) naphthol yellow S, and indigo carmine by indirect Anson's method using gel filtration. From the experimental results, it was found that all dyes tested inhibit in some extent tryptic activity at the concentration of 10-3M, and the inhibition by xanthene dyes is especially strong. Consequently, the dosage of these food dyes is preferable to be limited for use as food additives. As one of the causes of inhibition, the formation of inactive complex compound of enzyme-dye was assumed. This assumption was proved to be valid from the spectrophotometric data of trypsin-rose bengal complex and its precipitation at high concentration.View full abstractDownload PDF (565K) -
HIDEO SAWADA1969 Volume 15 Issue 4 Pages 258-263
Published: August 30, 1969
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThe blood, internal organ and stomach-intestine content of bromvalerylurea ingested rabbit were extracted with acetone after lyophilization, whereas the urine was extracted with ether at pH 7.0 by Kutscher-Steudel Instrument. Each extract was concentrated under reduced pressure, and chromatographed on thin-layer plate using chloroform-acetone (9 : 1) and chloroform-methanol (9 : 1), and detected by KI-benzidine reagent. The Rf values and color reactions observed were as follows, bromvalerylurea (0.38, 0.73, dark violet), 3-methylbutyrylurea (0.20, 0.66, dark violet), and urea (0, 0.03, orange). Bromvalerylurea and its metabolite in rabbit were analysed at intervals by the procedure described above. Both bromvalerylurea and 3-methylbutyrylurea were detected after 1-22 hours from stomachintestine content, internal organ (brain, little brain, spinal cord, liver, kidney, spleen, lung, bile), blood and after 48 hours, only 3-methylbutyrylurea from small intestine content, liver, kidney, spleen, lung, brain and urine. After 122 hours, no metabolite was found in almost all organs. Thus, it was recognized the bromvalerylurea was metabolized rapidly.View full abstractDownload PDF (977K) -
KOKU MATUMOTO, MASATOSHI INOUE, TADASHI FUJITA, HIROMI TERADA, TOMOKO ...1969 Volume 15 Issue 4 Pages 264-267
Published: August 30, 1969
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThe present investigation was undertaken to apply theoretical methods to the ventilation of polluted air in room where carbondioxide was not homogeneously diffused. C is the concentration of carbondioxide at time nδT (=pδt), and subscripts m-1 (=q-2), m-1/2 (=q-1), m (=q), m+1/2 (=q+1), and m+1 (=q+2) denote spaces on one dimention in room (m-1) δX [=(q-2) δx], (m-1/2) δX [=(q-1) δx], mδX (=qδx), (m+1/2) δX [=(q+1) δx], and (m+1) δX [=(q+2) δx], respectively, and superscripts +, 1/2, -1/2, and - denote time (n+1) δT [=(p+2) δt], (n+1/2) δT [=(p+1)δt], (n-1/2) δT [=(p-1) δt], and (n-1) δT [=(p-2) δt], respectively, where δX/2=δx, δT/2=δt, Assuming that the concentration of carbondioxide depends on time and space as described in the previous paper, a following equation can be obtained : 〓C/〓T=D (〓2C/〓X2) where X is a distance from a source of evolution of carbondioxide, and D is a constant (not equal to 0). According to a implicit difference analogue for the above equation, Taylor's expansion theorem, Crank-Nicolson's method, Schmidt's method, Dusinberre's method and D δt/(δx)2=1/2, following equations can be obtained : [numerical formula] [numerical formula] [numerical formula] [numerical formula] It was found that these methods were available for the theoretical study of natural ventilation of polluted air diffused heterogeneously in room.View full abstractDownload PDF (471K)
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