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Volume 26 (1980)
- Issue 6 Pages 275-
- Issue 5 Pages 205-
- Issue 4 Pages 145-
- Issue 3 Pages 107-
- Issue 2 Pages 55-
- Issue 1 Pages 1-
Volume 26, Issue 3
Displaying 1-7 of 7 articles from this issue
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KENICHIRO MORI, SHIGEKAZU NISHIDA, HIROFUMI HARADA1980 Volume 26 Issue 3 Pages 107-111
Published: June 30, 1980
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSMicroanalysis dealing with microgram level of ethyleneoxide (EO) is described. In this method, a florisil column prepared with hydrobromic acid was used as a reaction column to convert EO into ethylenebromohydrine (EBH). Samples, medical devices such as disposable syringe and foods such as pepper, dissolved in ethanol were heated in a water bath at 80°. EO expelled from the ethanol solution with N2 gas by N2-bubbling was introduced into the reaction column. EO in an air was collected in a sampling bag and directly introduced into the column by suction from the exit of the column. After EO was introduced, EBH formed in the column was eluted with ether, and the eluate was concentrated. EBH was determined by gas chromatography equipped with an electron capture detector. By this procedure, 0.1 ppm of EO in medical devices and foods, and 0.5 ppm in atmospheric air can be determined at about 3% of coefficients of variation without interference of co-existing organic substances.View full abstractDownload PDF (613K) -
HIDEO ISONO, HIROSHI KOZUKA1980 Volume 26 Issue 3 Pages 112-117
Published: June 30, 1980
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSA compound having a molecular formula C6H10O2N2 (MW 142, mp 218°) has been known to be formed by warming α-bromoisovalerylurea (BV) with 28% aqueous ammonia. In order to determine the chemical structure of this compound, the analytical data of the compound obtained by treatment of BV with ammonia were compared with those of synthetic 5-isopropylhydantoin (C6H10O2N2, MW 142, mp 145-146°) and 3-methylcrotonylurea (C6H10O2N2, MW 142, mp 213°) formed by heating BV with pyridine. From the above results it was clarified that the compound was different from synthetic and pyridine-treated compounds in infrared (IR) spectrum, nuclear magnetic resonance (NMR) spectrum, and thin-layer chromatogram. By more detailed analyses, this compound was determined to be 2-imino-5-isopropyl-4-oxazolidinone (OZ).View full abstractDownload PDF (634K) -
TATSURO MIYAHARA, TATSUO SAKAI1980 Volume 26 Issue 3 Pages 118-122
Published: June 30, 1980
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSWhen washed cells of Kloeckera apiculata were suspended in n-butyl p-hydroxybenzoate solution, release of α-amino nitrogen from the cells into the medium was observed. There was also a release of thiamine into the medium on incubation of thiamine-accumulated cells with n-butyl p-hydroxybenzoate. A close correlation between the extent of leakage and the percentage of dead cells was found. The cell uptake of thiamine was inhibited at a concentration of n-butyl p-hydroxybenzoate that caused no release of α-amino nitrogen. Formation of protoplasts on incubation of the benzoate-treated cells with lytic enzyme was suppressed in comparison with intact cells. Protoplasts were lysed by n-butyl p-hydroxybenzoate on incubation of the protoplasts with the benzoate in 0.8M NaCl solution. In view of these results, it seems possible that n-butyl p-hydroxybenzoate attacks the cytoplasmic membrane of yeast.View full abstractDownload PDF (557K) -
KAZUO SAITO, RYUZO TAKESHITA1980 Volume 26 Issue 3 Pages 123-128
Published: June 30, 1980
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSA method to collect non-polar organic air pollutants and concentrate them by using an adsorbent, dodecylsulfonic acid binding polyamide, was investigated. Air samples containing PCBs, phthalates and chloronaphthalene were passed through a column packed with the adsorbent and the compounds adsorbed were eluted with ethanol. The compounds eluted were extracted with hexane after dilution of the eluate with water and measured by gas-liquid chromatography with an electron capture detector. These compounds at low level were quantitatively collected by the adsorbent and eluted from the column. This method was applied to the determination of air pollutants.View full abstractDownload PDF (709K) -
RYOJI SAWAMURA, MARIKO TACHIKAWA1980 Volume 26 Issue 3 Pages 129-135
Published: June 30, 1980
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSNitrogen trichloride (NCl3) is considered as a material with unpleasant odor and causing eye irritation in swimming pool. The formation of NCl3 depends on pH and molecular ratio of chlorine to ammonia. When NCl3 was measured by Palin's neutral ortho-tolidine method with oxalic acid, false NCl3 not extracted with CCl4 was observed in the determination of chlorine solution containing a certain organic nitrogen compound such as isocyanuric acid (ICA), hypoxanthine, and dimethyl urea. The false NCl3 disappeared by lengthening the contact time with oxalic acid. In order to measure NCl3 without influence of coexistence of these compounds, NCl3 was extracted with CCl4, and the chlorine of NCl3 in CCl4 layer was mesured by iodometric method. By use of this CCl4-extraction method, the effect of ICA on the formation of NCl3 from ammonia and chlorine was studied. The quantity of NCl3 formed decreased by the addition of ICA. Isocyanuric acid had the same effect even when ammonia in solution was in the chemical form of monochloramine (NH2Cl), and dichloramine (NHCl2). When the dose of chlorine was less than that of breakpoint, ammonia in solution was decomposed without producing NCl3. Thus the separate addition of chlorine gave a practical effect on the decrease of NCl3 in the procedure of breakpoint chlorination.View full abstractDownload PDF (758K) -
HISAYA TERADA, TOSHIKATSU ISHIHARA, YOSHIO SAKABE1980 Volume 26 Issue 3 Pages 136-139
Published: June 30, 1980
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSA simple and rapid method for the determination of nitrate in vegetables by high-performance liquid chromatography was investigated. Nitrate was extracted with distilled water from vegetable samples. The sample solution was injected on a "Zipax"SAX column, a pellicular type anion exchange column, and eluted with 0.05M potassium dihydrogen phosphate solution. The eluent was monitored with a ultraviolet detector at a wavelength of 210 nm. The time required for the analysis was 10 min. The recovery of nitrate from ten kinds of vegetables was 86.0-102.0%.View full abstractDownload PDF (478K) -
TADASHI KOZU, YOICHI HOSOI1980 Volume 26 Issue 3 Pages 140-143
Published: June 30, 1980
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSMethamphetamine in cosmetic cream was determined by high-performance liquid chromatography with a UV detector by use of stainless column (4.0 mm×0.15 m) packed with Nucleosil 5C18. Mobile phase was 0.06 M NaH2PO4·methanol (85 : 15), adjusted to pH 2.2 with H3PO4. Methamphetamine in the sample was mixed with n-hexane and extracted with water. Determination was carried out successfully by using ephedrine as an internal standard and by maintaining the temperature at 20°. The recovery of methamphetamine added to cosmetic cream was 97.5%. The content of methamphetamine detected in a sample of illegal case was 5.9% by weight.View full abstractDownload PDF (461K)
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