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Volume 40, Issue 1
Displaying 1-21 of 21 articles from this issue
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Yoshie KOHNO, Toshiko TODA, Kunio WAKITA, Masakuni TAKAHASHI, Takashi ...2001 Volume 40 Issue 1 Pages 1-3
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSObjective: We studied the significance of uterine cervical cytology in teenagers.
Study design: Subjects were 164 teenagers visiting the Matsue Seikyo Hospital between 1993 and 2000. Papanicolaou smears were obtained from all subjects.
Results: Of the 164 subjects, 12 (7.3%) showed abnormal cytology; 11 of the 12 were assessed as Class III a, and the remaining 1 Class III b. We studied the characteristics and clinical courses of these 12 patients.
Conclusions: Cytology of the cervix in teenagers is important in cancer screening, because abnormality rates are rather high. Abnormal smears from sexually active women may be altered by influences from inflammations, including those from sexually transmitted diseases (STDs).View full abstractDownload PDF (2544K) -
Motoi SASAGAWA, Nobumichi NISHIKAWA, Seiji TSUKADA, Shigeru HONMA, Tak ...2001 Volume 40 Issue 1 Pages 4-8
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSObjective: We conducted a retrospective study to clarify the significance of cervical cytology in the diagnosis of endometrial carcinoma.
Study design: A clinical file of 184 cases with endometrial carcinoma was reviewed. A positive rate in preoperative cervical cytology was calculated and factors correlated with the positive rate were analyzed. The significance of normal endometrial cells detected by preoperative cervical cytoloy was assessed.
Results: Cervical cytology showed suspicious or malignant results in 81 cases (44.0%). The positve rate was significantly associated with the clinical stage and preence of atypical genital bleeding but was not influenced by histological type, histological grade, parity, the state of menopause, or peritoneal cytology. Normal endometrial cells were detected in 12 of 50 cases in which cytological materials were reexamined. Smears were taken on days 1-13 of the cycle in 1 case, after day 14 of the cycle in 1, postmenopausally in 8, and on an unknown day in 2. Tumor diathesis was observed in 8 cases.
Conclusion: Although cervical cytology primarily represents a screening modality for cervical disease, it suggests the presence of endometrial carcinoma in nearly half of all cases. The presence of endometrial cells and tumor diathesis on cervical cytology may predict endometrial lesions, particularly endometrial carcinoma.View full abstractDownload PDF (666K) -
Mitsuhiro NAKAMURA, Yoshiaki IMAMURA, Tadanori YAMAGUCHI, Takako KAWAD ...2001 Volume 40 Issue 1 Pages 9-14
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: We report 2 cases of papillary squamous cell carcinoma (PSCC) of the uterine cervix, a rare variant of squamous cell carcinoma.
Cases: Case 1 was a 69-year-old woman with an exophytic tumor 5 cm in diameter in the uterine cervix. Case 2 was a 47-year-old woman with a papillary tumor 4 cm in diameter in the cervix. Cytologically, both tumors featured papillary clusters of atypical small round cells with fibrovascular cores. Numerous mitotic figures were also seen. The degree of nuclear atypism was mild in case 1 and moderate to severe in case 2, in whom we observed, large vacuolated dysplasia-like cells and transitional cell carcinoma-like cells. Histologically, both tumors consisted of papillary architectures with fibrovascular cores lined by multilayered atypical cells. Immunohistochemically, they were positive for cytokeratin 7 (CK 7) but negative for CK 20, indicating squamous cell carcinoma of the uterine cervix. Human papillomavirus (HPV) was negative in in situ hybridization (case 1) and PCR (case 2).
Conclusion: Because cytological findings of PSCC vary with the case, histological and immunohistochemical findings are needed in addition to clinical findings for precise diagnosis.View full abstractDownload PDF (8741K) -
Yusuke MATSUURA, Mayumi TANAKA, Masamichi KASHIMURA, Akira SHIRAHATA2001 Volume 40 Issue 1 Pages 15-19
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: The incidence of cervical cancer or cervical intraepithelial neoplasia (CIN) increases in patients infected by the human immunodeficiency virus (HIV), although few such cases have been reported in Japan.
Case: A 28-year-old Japanese woman with HIV infection was referred to our department after becoming pregnant. Evacuation was conducted due to a low CD 4-positive lymphocyte count. Cervical cytology showed dyskaryotic cells with mild nuclear atypia. Colposcopic impression was dysplasia, and punch biopsy of the uterine cervix revealed mild dysplasia. Human papillomavirus (HPV) DNA was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). She received combination antiretroviral therapy, and CD 4 increased. She had an elective cesarean-section delivery at age 31. Cervical cytology and colposcopy showed no evidence of dysplasia and HPV DNA was negative after delivery.
Conclusion: Cervical cancer screening is recommended for all women with HIV infection. Sexually transmitted disease (STD) including HPV should also be checked for.View full abstractDownload PDF (3422K) -
Hiroko NAKAHARA, Junko KAYAO, Tadashi SAITO, Jo ISHII, Kazuhiro TOMARU ...2001 Volume 40 Issue 1 Pages 20-26
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Cytomorphological diagnosis of adenoma malignum, a subtype of endocervical adenocarcinoma, is often difficult because the tumor cells closely resemble benign endocervical cells. We describe 2 cases of adenoma malignum diagnosed by endocervical scraping cytology.
Case: Two Japanese women aged 48 and 41 reported abnormal vaginal discharge. Adenoma malignum or its related tumors were strongly suspected from pelvic examinations, endocervical scraping cytology, and conization of the uterine cervix. In both, tumor cells from endocervical scraping were mucin-producing cells with minor cellular or nuclear atypia and a few cell clusters resembling endocervical adenocarcinoma. Histological examination of the resected uterus revealed atypical hyperplastic mucous glands invading to the profound layer. Most tumor cells were positive for HIK 1083 antibody and PAS staining. These cells demonstrated a decreased reaction to alcian blue and high iron diamine-alcian blue.
Conclusion: For the cytological diagnosis of adenoma malignum, we found the staining property of mucin and its histochemical findings are sufficient for differentiation. The correct diagnosis is made with the aids of relevant clinical information.View full abstractDownload PDF (3854K) -
Takao MASUDA, Tamami KUDO, Jun KUWABARA, Yukio ISHIKAWA, Yoshikiyo AKA ...2001 Volume 40 Issue 1 Pages 27-31
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Ovarian strumal carcinoid is a germ-cell tumor characterized by an intimate mixture of thyroid tissue and carcinoid tumor. We describe a case of ovarian strumal carcinoid diagnosed by imprint cytology.
Case: A 64-year-old woman reporting abdominal distension was diagnosed with bilateral ovarian teratomas based on computed tomography (CT) of the pelvis. Imprint cytology material from the right ovarian tumor revealed numerous tumor cell clusters composed of small, round cells forming rosette-like or trabecular structures. Irregularly shaped eosinophilic substances scattered in the background were considered to be thyroid tissue colloids. These cytologic features suggested that the right ovarian tumor was ovarian strumal carcinoid. Histologically, the tumor was positive in Grimelius' staining and positively immunostained with 2 antibodies for thyrogloulin and chromogranin A.
Conclusion: Imprint cytology is useful in diagnosing ovarian strumal carcinoid, in addition to histological examination.View full abstractDownload PDF (5986K) -
Naruaki MATSUI, Shigeki MIYANAGA, Aki KAWAHARA, Takashi KITAMURA, Masa ...2001 Volume 40 Issue 1 Pages 32-37
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Carcinoid and small-cell carcinoma are known as neuroendocrine tumors in primary pulmonary carcinoma. The current World Health Organization (WHO) classification includes a new independent category of large-cell neuroendocrine carcinoma (LCNEC), that is morphologically and biologically an intermediate degree of malignancy between carcinoid and small-cell carcinoma. We detail cytological findings in 2 cases of LCNEC we observed.
Cases: Subjects were 2 men-one 68 years old and the other 71 years-old. Cytological findings showed large and small cell groups including rosette-like structures and filamentous groups. Many tumor cells were oval or spindle-shaped. Tumor cells showed a high N/C ratio, and had slight lightgreen staining or granular cytoplasms. Nuclei were oval, and each nucleus was located in the center of the cell. A thin nuclear membrane and fine chromatin were observed. Nuclear lines were rare or absent.
Conclusion: Cytological findings for our 2 subjects were similar to those in conventional reports in the size of tumor cell, nuclei, and cell groups. Cytological findings of oval nuclei, a thin nuclear membrane, fine chromatin, and the rarity or absence of nuclears lines in the background are considered diagnostically important.View full abstractDownload PDF (8318K) -
Shin-ichi SHIMIZU, Masahiro ASANO, Tetsuji YAMADA, Yurika SAWAKI, Mako ...2001 Volume 40 Issue 1 Pages 38-41
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Oncocytic tumors of the salivary gland are rare and few cytological reports are available. We describe characteristic cytological, histological, and electron microscopic features in a case of oxyphilic adenoma arising in the right parotid gland.
Case: A 70-year-old woman reported a gradually growing mass in the right parotid region. Imprint cytology preparation contained tumor cells arranged in single and cohesive clusters of different sizes. Tumor cells had ample eosinophilic granular cytoplasm. Nuclei were round and eccentric in some tumor cells and occasionally had prominent nucleoli. Apparent nuclear atypia, overlapping nuclei, and mitosis were not be identified. Histologically, the tumor showed a proliferation of oncocytic cells, mainly in nests partly with glandular formation. Tumor invasion of the capsule or surrounding tissue was not seen. Electron microscopic examination also confirmed tumor cells had numerous mitochondria occupying the entire cytoplasm. Based on these findings, we diagnosed the tumor as an oxyphilic adenoma.
Conclusion: The cytological features detailed above are highly suggestive of oncocytic tumors. Even though no evident nuclear atypia or brisk mitosis was seen in cytological specimens, further histological examination is required to confirm differentiation between oxyphilic adenoma and carcinoma.View full abstractDownload PDF (6408K) -
Akihiko KAWAHARA, Toshiro YOKOYAMA, Hideyuki ABE, Tomohiko YAMAGUCHI, ...2001 Volume 40 Issue 1 Pages 42-47
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Salivary duct carcinoma (SDC) is a rare salivary gland tumor mainly arising from the parotid gland. Reports on the cytological features of SDC are few. We report a case of SDC arising in the parotid gland and describe the cytological features using imprinted cytologic specimens.
Case: A 71-year-old Japanese man. He underwent resection after computed tomography (CT) and magnetic resonance imaging (MRI) revealed an irregularly bordered 5.0×2.5 cm tumor. Imprint cytology showed large sheet clusters with slight overlapping and scattered polygonal cells with abundant cytoplasm in the necrotic background. Tumor cells varied in form and cytoplasm staining, which is often observed in intranuclear inclusion and cytoplasmic valuoles. Histologically, tumor cells showed various invasive growth in cystic, solid, papillary, and cribriform patterns. Some cells also had apocrine-like features.
Conclusion: The cytological features of SDC require careful examination to differentiate them from mucoepidermoid carcinoma, oncocytic neoplasms, and metastatic adenocarcinoma. In determining SDC, it is vital to observe cell size and variations in cellular morphology.View full abstractDownload PDF (9444K) -
Seiji TSURUTA, Yutaka NOMOTO, Chiharu SHINBO, Harumi NEGISHI, Misa IIJ ...2001 Volume 40 Issue 1 Pages 48-52
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Malignant lymphoma, Enteropathy type intestinal T-cell lymphoma (ETCL) is a rare primary intestinal disorder, paticularly in Japan. We report a case of ETCL arising in the duodenum.
Case: A 51-year-old Japanese man presented with malabsorption syndrome, including hypoproteinemia. An ulcerating mass in the duodenum was resected. Intraoperative cytological specimens of the duodenal tumor contained numerous atypical medium to large lymphoid cells associated with a background of histiocytes. On Papanicolaou (Pap) stain, tumor cells had moderate amounts of amphophilic cytoplasm. Their nuclei were round or slightly indented, with a thick nuclear membrane, containing multiple small but conspicuous nucleoli situated at the nuclear membrane and finely dispersed chromatin. Scattered mitoses were also observed. Giemsa-stained specimens revealed numerous intracytoplasmic azurophilic granules. Based on histological examination of surgical materials, including immunohistochemical analysis, we confirmed ETCL.
Conclusion: Giemsa-stained cytology specimens are informative when dealing with ETCL because azurophilic granules are easily identified in the cytoplasm of variable proportions of tumor cells. This feature, however, could not be determined in Pap-stained specimens.View full abstractDownload PDF (6390K) -
Yoshinari TANAKA, Akiyoshi KAMIHARA, Kouji AZUMA, Isao SHIMOKAWA, Masa ...2001 Volume 40 Issue 1 Pages 53-57
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: We present a case of intraductal carcinoma showing cytologic features of apocrine carcinoma. Cytologic findings are reported comparing apocrine carcinoma cells and benign apocrine-metaplastic cells.
Case: A 74-year-old woman reported pain in the right breast. Clinical examination revealed a nodule in the right breast C-area. Needle-aspiration specimens showed scatteredisolated cells and loosely packed clumps of cells against a necrotic background. Enlarged, pleomorphic nuclei overlapped and crowded in cell aggregates. The cell border was indistinct, and finely granular or foamy cytoplasm exhibited eosinophilia. These cytologic features suggested apocrine carcinoma. Histologic examination confirmed these findings, although the diagnosis was noninvasive ductal carcinoma.
Conclusion: Nuclear atypism, loose packing, cell clumping, and an indistinct cell boundary indicate malignant apocrine-metaplastic cells. Cytologic findings are important in differentiating apocrine-metaplastic cells, since apocrine metaplasia is thought to be benign.View full abstractDownload PDF (3316K) -
Yuji MORI, Toshihito SHINAGAWA, Fumikazu KIMURA, Ayako SUZUKI, Megumi ...2001 Volume 40 Issue 1 Pages 58-62
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Neuroendocrine carcinoma (NEC) of the prostate is rate, with the few cases known found by urinary cytology.
Case: A 78-year-old man with pollakiuria was found to have well differentiated adenocarcinoma of the prostate by needle biopsy. Urinary cytology at that time suggested probable adenocarcinoma cells. After 3 years and 5 months, the patient underwent (TUR) due to dysuria. Histologically, tumor cells revealed NEC features with an adenocarcinoma component. Postoperative urinary cytology exhibited exclusively small cells with naked nuclei, scattered and occasionally forming small clusters arranged in single file. Tumor cells featured a high N/C ratio, hyperchromatic nuclei, and scanty cytoplasm. Immunohistochemically, NEC tumor cells were positive for neuron-specific enolase, synaptophysin, and chromogranin A. Adenocarcinoma cells were positive for prostatic-specific antigen and prostatic acid phosphatase.
Conclusion: In the daily screening of urine cytology in prostatic adenocarcinoma follow-up, it is important to remember that even typical adenocarcinoma may undergo NEC differention.View full abstractDownload PDF (8984K) -
Rieko MINAMI, Yuko NISHIKAWA, Mikako OHYA, Ryuko TSUKAMOTO, Mayumi TAC ...2001 Volume 40 Issue 1 Pages 63-70
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Neuroendocrine carcinoma (NEC) is a rare neoplasm of the urinary bladder accounting for less than 0.5% of all bladder malignancies. We clarified the characteristics of neuroendocrine carcinoma cells in voided urine.
Case: Urinary cytology was reviewed in 4 patients-1 man and 3 women (mean age: 64)-with NEC in urinary bladder. All had hematuria, and underwent surgery at Kobe University Hospital and affiliated institution. In cases 1-3, urinary specimens harbored numerous small tumor cells with a high nuclear/cytoplasmic ratio. The nuclear chromatin was fi ne and granular and nucleoli inconspicuous. Tumor cells were arranged in singly or in clusters, and nuclear molding, single-file, or rosette-like arrangements were identified. Both transitional cell carcinoma and adenocarcinoma were observed in case 3. In case 4, relatively uniform small, round tumor cells were singly dispersed, loosely attached, or in small aggregates. Chromatin was coarse and granular and nucleoli small but conspicuous. Histopathologically, in cases 1-3, tumors consisted of small undifferentiated cells with oval hyperchromatic nuclei and scant cytoplasm. In case 4, monotonous small round tumor cells with eosinophilic and fine granular cytoplasm infiltrated solidly, penetrating the bladder wall. Histochemical, immunohistochemical and ultrastructural analysis confirmed the diagnosis of NEC in the urinary bladder.
Conclusion: The accurate diagnosis of NEC in the urinary bladder is made based on the urine cytologic features described above, making the role of urine cytology very important in early detection of such highly malignant tumors.View full abstractDownload PDF (9925K) -
Tomoko YAMAMOTO, Kiyotaka WAKUI, Toshiko KANAMURO, Yuji NONAMI, Noriyu ...2001 Volume 40 Issue 1 Pages 71-75
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Malignant pheochromocytoma cytology is reported in a very rare case showing morphologic alteration and increased proliferation following onset.
Case: A 59-year-old man diagnosed with pheochromocytoma eventually died of repeated tumor recurrence over a period of 34 years. Histologically, tumor cells became small and uniform with decreasing granular cytoplasm and higher indices of cell proliferation and death one year prior to the patient's death. Stump specimens from a tumor at autopsy showed small tumor cells with prominent nucleoli and faintly granular cytoplasm. Some cells exhibited nuclear inclusion. Anisonucleosis was not conspicuous and occasional mitotic figures were seen.
Conclusion: Cytological findings in our case are representative of malignant pheochromocytoma with high growth. Occasional mitoses and the small, uniform appearance of tumor cells may help in cytologically diagnosing malignancy in pheochromocytoma.View full abstractDownload PDF (5738K) -
Reiji HABA, Shoji KOBAYASHI, Hiroshi MIKI, Yoshio KUSHIDA, Kousuke SAO ...2001 Volume 40 Issue 1 Pages 76-80
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Malignant gastrointestinal tumor (GIST) arising in the omentum is very rare, and its cytological findings have not been reported.
Case: A 73-year-old man reported right upper abdominal pain. Ultrasonography, compurted tomography (CT), and magnetic resonance imaging (MRI) showed a huge mass in the left upper abdomen and 2 cystic tumors in the liver. Chemotherapy was conducted with 5-FU and CDDP, and the patient died 9 months later. At autopsy, the tumor, 22.3×16.0×7.0cm in size, was found to arisen in the omentum. Cytology revealed solitary spindle and polygonal cells with rich cytoplasm. The nucleus was round and oval, with nuclear anisokaryosis and irregularity marked, and chromatin pattern finely granular. Multinucleated cells with a giant nucleus and intracytoplasmic inclusions were also observed. Histologically, tumor cells had rich cytoplasm, proliferating in sheet or fascicular patterns with hypervascular stroma. Immunohistochemically, tumor cells were positive for CD 34, CD 117, and vimentin.
Conclusion: Diagnosis of GIST by cytology alone is difficult, but it is possible to diagnose malignancy from cytological findings such as cytological atypia, cellularity, necrotic background, and tumor size.View full abstractDownload PDF (6812K) -
Akiko ADACHI, Kou KANEKO, Mitsuhiro NOKUBI, Kenji MITA, Kinuyo ISAYAWA ...2001 Volume 40 Issue 1 Pages 81-84
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: We report a rare case of ectopic orbital meningioma, in which intraoperative scraping cytology was very useful in diagnosis.
Case: A 54-year-old man was admitted to our hospital after suffering from progressive left-sided ptosis 3 years. A mass lesion was palpated on left upper eyelid and computed tomography (CT) and magnetic resonance imaging (MRI) showed a 1.5cm mass lesion on the antero-superior aspect of the left levator palpebrae. The mass was resected when a frozen section showed an epithelial structure suspicious as invasion of a malignant epithelial tumor. Scraping cytology clearly showed clusters of benign cells with a whorl arrangement and intranuclear cytoplasmic intrusion (pseudoinclusion) suggestive of meningioma. Permanent histological specimens and immunohistochemical studies confirmed the diagnosis of meningotheliomatous and transitional meningioma.
Conclusion: We treated a rare case of intraorbital ectopic meningioma in diagnosis by frozen section alone was not possible. Scraping cytology examination led us to correct the diagnosis and underscored the usefulness of intraoperative cytological examination.View full abstractDownload PDF (5595K) -
Yoshihito YOKOYAMA, Tomomi SAKAMOTO, Hidetoshi MARUYAMA, Shigemi SATO, ...2001 Volume 40 Issue 1 Pages 85-86
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe report a patient with primary non-Hodgkin's lymphoma of the uterine cervix diagnosed by a uterine cervical smear that demonstrated abnormal lymphocytes. Tissue from the uterine cervix showed malignant diffuse largecell lymphoma. She experienced complete clinical remission following combined chemotherapy and irradiation therapy.View full abstractDownload PDF (4881K) -
Keiji SATO, Junji NAGAHAMA, Kenji KASHIMA, Tsutomu DAA, Shigeo YOKOYAM ...2001 Volume 40 Issue 1 Pages 87-88
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe report cytologic findings on a steroid lipid cell tumor of the ovary in a 45-year-old woman suffering from masculinization, including amenorrhea since the age of 41, hoarseness and hirsutism. Computed tomography revealed a right ovarian tumor. Serum androgenic hormones elevated before surgery returned to the normal range 10 days after the tumor was resected. An implint cytology specimen was composed of 2 types of tumor cell-those with abundant pale cytoplasm with occasional vacuoles and those with less cytoplasm stained well with light green reflecting the histological pattern of the tumor.View full abstractDownload PDF (2005K) -
Noriko HOSAKA, Akiko KOBAYASHI, Hirofumi SAKURAI2001 Volume 40 Issue 1 Pages 89-90
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSImprinting cytology using fresh brain materials obtained surgically from a 65-year male with a sparsely granulated pituitary adenoma revealed relatively large cells with inclusion bodies in the nuclei and severe pleomorphism. Other cells were small and round with granular cytoplasm and small granular nuclei. Immunohistochemically, growth hormone stained in cytoplasm and CAM 5.2 at the nuclear periphfery with a granular pattern, but findings for ACTH, PRH, CK, and EMA were negative. These findings on the brain species revealed a sparsely granulated adenoma of the pituitary gland, confirming the utility of cytologoly, in addition to histopathology, in diagnosing brain tumors.View full abstractDownload PDF (2413K) -
Rika KUSUMI, Yoshitaka TORII, Tadashi YAMAMOTO, Yasuo NAKATA, Hitoshi ...2001 Volume 40 Issue 1 Pages 91-92
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSCell transfer is applicable to broken slides and the preparation of multiple slides from a single smear on limited cytological material. We clarified the effectiveness of cell transfer by different mounting media and used resulting preparations to conduct immunocytochemical staining. Our results indicate that temperature and time in softening were affected by the properties (viscosity and xylene components) of mounting media. Cellular preservation and reactivity of most antigens are maintained following transfer. Cytological materials prepared by cell transfer are therefore suitable for immunocytochemical study.View full abstractDownload PDF (2370K) -
2001 Volume 40 Issue 1 Pages 93-98
Published: January 22, 2001
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (10652K)
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