巻号一覧
36 巻, 5 号
選択された号の論文の14件中1～14を表示しています
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分離能に及ぼす保存日数の影響宮本 正樹, 柴 雅之, 村 徹, 笹川 滋1990 年 36 巻 5 号 p. 567-573
発行日: 1990/10/30
公開日: 2010/03/12
ジャーナル フリーWe examined the effect of storage periods of CRC on leukocyte removal by Sepacell-R500 filter using fluorescence staining method to determine small numbers of leukocytes in filtrated CRC. The CRCs prepared from 400ml of whole blood and stored for 0, 2 and 10 days were passed through Sepacell-R500 by gravity or at a flow rate for blood transfusion (4ml/min) without priming the filter and suspending CRC with saline. Sepacell-R500 removed more than 99.9% of the leukocytes (the residual leukocyte numbers: less than 8×105) while 89-98% of red cells were recovered from one unit of CRC. There were no significant differences between the pre- and post-filtered red cells in cell volume, osmotic fragility, free hemoglobin contents in supernates, the levels of ATP and 2, 3-DPG and morphological change. The removal rates of leukocytes by the filter were unchanged between the CRC stored for 2 and 10 days. In the case of fresh CRC filtrated through by gravity, the leukocyte removal rate was 95-96% and the remaining leukocytes in the CRC were mostly granulocytes. The phenomenon was due to the higher flow rate and lower granulocyte adhesion than those of CRC stored for 2 and 10 days. On the other hand, the leukocyte removal rate was about 99.9% when fresh CRC was filtrated at the rate of 4ml/min. The filtration procedure at the patient's bedside was excellent for the prepration of leukocyte-poor CRC regardless of storage periods.抄録全体を表示PDF形式でダウンロード (885K) -
福井 弘, 西田 幸世, 藤村 吉博, 金田 美喜夫, 嶋 裕子, 吉岡 章, 吉原 なみ子, 中永 和枝1990 年 36 巻 5 号 p. 574-579
発行日: 1990/10/30
公開日: 2010/03/12
ジャーナル フリーThe antibodies against HIV-2, the alternative etiologic agent for acquired immunodeficiency syndrome (AIDS), were evaluated on a total of 121 Japanese hemophiliacs (106 hemophilia A, and 15 hemophilia B), using a combination of enzyme-immunoassay (ELAVIA-2), western blotting (LABBLOT 2), and peptide immunoassay (PEPTILAB1-2) provided from Pasteur-Sanofi, France. The twenty eight patients of hemophilia A (26%) and the three of hemophilia B (20%) showed positive results by ELAVIA-2. PEPTILAB 1-2 test specified that only one patient (case X) with hemophilia A has a double reaction to HIV-1 and HIV-2 specific peptides. LABBLOT 2 also demonstrated the immunoreactivity of case X to HIV-2 proteins; p26, gp36, gp105, and gp140. Case X was further examined using polymerase chain reaction (PCR) method to detect proviral DNA sequences of HIV-1 and HIV-2, and finally the possibility of HIV-2 infection was ruled out confirming true HIV-1 infection.抄録全体を表示PDF形式でダウンロード (2658K) -
福森 泰雄, 田中 成憲, 杉本 昭子, 大軒 子郎, 山口 英夫1990 年 36 巻 5 号 p. 580-586
発行日: 1990/10/30
公開日: 2010/03/12
ジャーナル フリーIrradiation with X-ray to blood products is a standard practice recommended for prevention of post-transfusion graft versus host disease in patients. In order to seek the optimal condition of irradiation to minimize the lesion of red blood cell (RBC) with complete inactivation of lymphocytes, we studied the effects of irradiation with X-ray on the quality of RBC in whole blood (WB) and concentrated red blood cells (CRC) in the range of 5 to 50 Gy.
X-ray irradiation did not alter ATP and 2, 3-DPG content, hemolysis ending point and morphology of RBC, while it caused a slight increase of osmotic pressure at hemolysis starting point of RBC and potassium concentration in plasma. Considerable increase of hemolysis was observed with higher dose of irradiation in both WB and CRC. However, it was so small as below 2-3×10-2 percent that is acceptable level in blood products.
Therefore, we concluded that the X-ray irradiation of WB and CRC with up to 50 Gy has no significant effects on in vitro characteristics of RBC.抄録全体を表示PDF形式でダウンロード (671K) -
光畑 裕正, 松元 茂, 大高 公成, 島田 友幸, 松岡 富男, 矢部 雅哉1990 年 36 巻 5 号 p. 587-592
発行日: 1990/10/30
公開日: 2010/03/12
ジャーナル フリーTo evaluate present status of surgical transfusion during operations in our operating room, we analyzed the amount of preoperative preparation of blood for an operation, and frequencies for transfusion in cases of various departments and kinds of operation using cross-match to transfusion ratio (C/T ratio). Also we evaluated the possibility to establish the national standard guideline of maximum surgical blood order schedule (MSBOS), and compared our trial of MSBOS with other MSBOSs reported based on both local and national levels.
Analysis with C/T ratio was very useful to recognize the present status of preparations of blood for surgery preoperatively. We noticed too many preparations of blood preoperatively in our operating room. We tried a trial of MSBOS based on our statistical data in our operating room. When we applied these data to 32 operations, which were analyzed in this study, we could save 80% of blood preoperatively compared with the present preparations of blood for those operations.
We considered that realistic C/T ratio is 2, 5, and it is better than 1.5 which is recommended by The Welfare Mimistry in Japan.
Guidelines of MSBOS in our hospital were similar to those of other institution reported at both local and national levels. Also we were not able to find differences between our MSBOS and reported those in Japan. We thought that national standard guidelines of MSBOS were valuable to apply to operations by various surgeons and in various hospitals. We therefore recommend that the Japan Society of Blood Tanasfusion make the national guidelines of MSBOS based on national-wide data.抄録全体を表示PDF形式でダウンロード (741K) -
大久保 進, 岡山 桂子, 石田 萠子, 安永 幸二郎1990 年 36 巻 5 号 p. 593-597
発行日: 1990/10/30
公開日: 2010/03/12
ジャーナル フリーThirteen patients having anti-P1 and nine having anti-Leb antibodies were followed up on their antibodies after transfusions of blood cell products bearing the corresponding antigens.
Before the transfusions, their antibodies mostly reacted only at the room temperature (RT), their titers being less than ×2. After the transfusions, three of the 13 anti-P1 and 4 of the 9 anti-Leb became reactive for the first time at 37°C, the titers elevating only up to twice in 4/13, and 4/9, respectively. However, one each anti-P1 and anti-Leb, which reacted at 37°C even before the transfusion, elevated up to ×32. In this anti-Leb case, conversion of the immunoglobulin class, i. e. from IgM to IgG, was observed, which was attributable to repeated platelet transfusions, seeming to warn us the possible side effects of transfusion. On the other hand, we found that 7/10 anti-Leb reacted at 37°C only after preincubation at RT, but not without it, indicating the false positive results of the ordinary pretransfusion tests at 37°C. Actually, no clinical side effects were detected in all of those 22 recipients. In addition, 6/13 of anti-P1 and 4/9 of anti-Leb disappeared after transfusions.
These findings obtained suggest that, for transfusions to the patients having such cold-reactive unexpected antibodies as anti-P1 or anti-Leb, selection of blood cell products compatible in this respect can be omitted.抄録全体を表示PDF形式でダウンロード (592K) -
村上 肇, 坪倉 兌雄, 天木 一太1990 年 36 巻 5 号 p. 598-602
発行日: 1990/10/30
公開日: 2010/03/12
ジャーナル フリーFour hundred milliliter of blood was collected from healthy donor in a quadrupl-bag system containing 56ml of citrate-phosphate-dextrose. Whithin 6 hours of collection, the blood was centrifuged at 1, 300×g for 6min in KUBOTA-9810 centrifuge. After centrifugation, the blood was separated using automated separator into platelet rich plasma, buffy coat fraction (approximately 40ml), and concentrated red cells.
Buffy coat fractions contained 72.8±8.4 percent of the leukocytes and 6.1±1.1 percent of the red cells of the original value. Thus, the number of leukocytes remainging in the buffy-coat-depleted red cells were 60±27×107 per unit, in paticular, lymphocytes decreased to 1.9±1.0×107 per unit with removal of 97.1±1.3 percent of the original value. Therefore, this method seems to be useful for preparation of leukocyte poor red cells component.抄録全体を表示PDF形式でダウンロード (1603K) -
中野 忠男, 棚町 啓之, 菅原 弘一, 伊東 盛夫, 佐分利 能生, 御手洗 義信, 友成 洋子, 田口 俊夫, 瀬尾 たい子, 大久保 ...1990 年 36 巻 5 号 p. 603-607
発行日: 1990/10/30
公開日: 2010/03/12
ジャーナル フリーA case of Tn-polyagglutination was reported. The patient, 60-year-old man, had pancytopenia and chronic liver function disturbances. His blood cells were polyagglutinable due to Tn-activation. The true blood group of the patient was B, but his cells had an acquired A-like specificity. In addition to the reactions of his red cells with various lectins, the nature of Tn antigen was analysed, using SDSPAGE, flow cytometry and Western blotting. Western blot study revealed that monoclonal anti-Tn reacted with α2, αδ, and β sialoglycoproteins.抄録全体を表示PDF形式でダウンロード (1469K) -
林 悟, 永峰 啓丞, 青地 寛, 押田 眞知子, 山本 一恵, 倉田 義之, 高原 史郎, 石橋 道男, 園田 孝夫1990 年 36 巻 5 号 p. 608-613
発行日: 1990/10/30
公開日: 2010/03/12
ジャーナル フリーA case of severe auto-immune hemolytic anemia after a living, ABO-incompatible renal transplantation is reported.
The recipient, a 34 years old man, blood group A1, Rh0 (D) positive receieved a renal transplant from father whose blood group O, Rh0(D) positive donor. Cyclosporine, prednisolone and mizoribine were used for immunosuppresion.
The recipient devloped severe immune hemolytic anemia within 2 weeks of renal transplantation. The recipient's serum contained anti-A1 IgG autoantibody and anti-B IgG antibody on day 18 post-transplantation. The IgG subclass of these antibodies was IgG3.抄録全体を表示PDF形式でダウンロード (660K) -
安田 広康, 八巻 淳子, 橋本 長吉, 遠山 ゆり子, 田崎 哲典, 大戸 斉, 阿部 力哉, 一瀬 裕子, 橋本 重厚1990 年 36 巻 5 号 p. 614-618
発行日: 1990/10/30
公開日: 2010/03/12
ジャーナル フリーThe blood of two patients were investigated for immune hemolytic anemia after administration of the following antibiotic drugs: Aspoxicillin (Asp. Doyle®) in the first case, Sodium Sulbactam/Sodium Cefoperazone (SBT/CPZ, Sulperazon®) in the second case.
Anti-Asp antibodies of the IgG1 subclass were found in the patient's sera and in the eluates of direct antiglobulin test (DAT) positive red blood cells in the first case, therefore we suggested that hemolysis was caused by anti-Asp antibodies reacting by a well-known ‘hapten-type’ drug absorption mechanism.
The other, the autologous red blood cells in the second case was positive by DAT, but only with anti-C3d reagents, and when the patient's serum was incubated (2 hrs at 37°C) with enzyme-treated red blood cells in the presence of the drugs (SBT-CPZ) and complement, hemagglutination was obserbed by indirect antiglobulin test (IAT). It was interesting that, at the time of these findings, the patient's serum reacted weakly with SBT-coated red blood cells but not with CPZ-coated red blood cells by the flow cytometric analysis. These would suggest that so-call ‘immune complex’ mechanism and another mechanism (perhaps drug absorption mechanism) were involved. It is probable that one or both of these mechanisms were responsible for the patient's immune hemolytic anemia in the second case.抄録全体を表示PDF形式でダウンロード (534K) -
Irena Sniecinski1990 年 36 巻 5 号 p. 619-627
発行日: 1990/10/30
公開日: 2010/03/12
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二之宮 景光1990 年 36 巻 5 号 p. 628-631
発行日: 1990/10/30
公開日: 2010/03/12
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1990 年 36 巻 5 号 p. 632-651
発行日: 1990/10/30
公開日: 2010/03/12
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1990 年 36 巻 5 号 p. 652-666
発行日: 1990/10/30
公開日: 2010/03/12
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1990 年 36 巻 5 号 p. 667-670
発行日: 1990/10/30
公開日: 2010/03/12
ジャーナル フリーPDF形式でダウンロード (537K)
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