In order to avoid a wide variety of side effects associated with blood transfusion such as nonhemolytic febrile transfusion reactions (NHFTR), HLA-sensitization and Cytomegalovirus infection. Leukocyte filters have come into wide use in bedside blood transfusion in Japan. During the storage of blood components, however, residual leukocytes disintegrate and release various biologically active substances, which constitute one of the causes of transfusion-related adverse events. Therefore, removal of leukocytes immediately or at an early stage after blood collection is expected to decrease the incidence of transfusion-related side effects by controlling the deterioration of blood cells or preventing the accumulation of cytokines.
The WBF2 closed-bag system is a blood collection-component separation system designed for the removal of leukocytes from whole blood and the preparation of blood components by centrifugation. In the present study, we evaluated the usefulness of the system in the preparation of leukocyte-reduced RC-MAP and FFP using WBF2, and compared the quality of these preparations with those by conventional post-storage leukoreduction or non-leukoreduction. The WBF2 system yielded red cell components with superior red cell recovery and lower accumulation of some cytokines, demonstrating its suitability for use in a blood center program. It was also noted that there was almost no macroaggregation in red cell components prepared by prestorage leukoreduction.

抄録全体を表示

To prevent the post-transfusion reactions in transfusion therapy such as HLA alloimmunization, non-hemolytic febrile transfusion reaction (NHFTR) and Cytomegalovirus transmission, bedside leukocyte reduction has been performed. However, it has become clear that prestorage leukocyte reduction has greater efficiency than poststorage, and prestorage leukocyte reduction is now being considered for implementation. We developed a prestorage leukocyte reduction system that contains CPD anticoagulant solution (PLD-C system). In this study, the performance of the PLD-C system was examined with regard to the leukocyte filtration efficacy and the characteristics of blood during storage by comparison with a pre-storage leukocyte reduction system which uses ACD-A solution (PLD-A system) and blood components collected using CPD solution and prepared by the buffy-coat depleted method (BCPD).
It was confirmed that the leukocyte reduction ratio of the PLD-C system (more than-4log) was higher than that of the BCPD (less than-0.4log). Platelet depletion rates of the PLD-C group (average more than 99%) was also higher than in the BCPD group, but the red cell recovery ratio was the same between groups (average 88%). Prestorage filtration showed no adverse effects such as hemolysis, whereas various good effects were observed, such as inhibition of macroaggregate formation and hemolysis during storage. Collecting blood into CPD solution improved red cell quality during storage and increased plasma volume compared to the ACD-A solution.
It was confirmed that the PLD-C system has advantages in the production of blood components over the present system. Use of this system can be expected to prevent post transfusion reactions.

抄録全体を表示

We evaluated the practical education of 5^th grader medical students at a regional blood center concerning various aspects of blood transfusion.
Practical education consisted of observation of voluntary blood donation, assistance in publicity of blood donation center, learning how to collect and prepare various blood products free of contami nation with infectious agents, contamination, performing blood typing as well as cross-matching and viewing short video lectures to avoid blood transfusion mismatches. The program has begun from 1987. Fifth grader students were surveyed to determine the effectiveness of this program at the end of the school year. Most students responded that they had improved their understanding of blood transfusion; in particular, they realized that blood products are obtained through the combined big efforts of volunteer donors and blood center workers. They also recognized that, as future doctors, they must minimize the use of blood products to avoid possible adverse side effects and also to ensure self-sufficiency of blood products in Japan. The level of understanding achieved through this practical program in the 5^th grader was markedly better than that achieved by lectures to students in the first 3^rd grader of medical school.

抄録全体を表示