巻号一覧
44 巻, 6 号
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別所 正美1998 年 44 巻 6 号 p. 675-686
発行日: 1998/12/01
公開日: 2010/03/12
ジャーナル フリーPDF形式でダウンロード (1524K) -
佐藤 典宏, 吉川 義洋, 茂木 祐子, 上田 恵里奈, 池淵 研二, 花谷 馨, 山田 秀人, 藤本 征一郎, 関口 定美1998 年 44 巻 6 号 p. 687-692
発行日: 1998/12/01
公開日: 2010/03/12
ジャーナル フリーWe investigated the effect of the removal of cryoprotectant after thawing of cryopreserved cord blood cells on their recovery rates. Cord blood was obtained at normal delivery.
Removal of red blood cells and the concentration of white blood cells in cord blood were performed by use of hydroxyethyl starch. As cryoprotectants, dimethyl sulfoxide and dextran were added at the final concentration of 10% and 1%, respectively. After 3-6 months preservation in liquid nitrogen, cord blood units were thawed, and an equal volume of dextran/albumin solution was added as washing medium. After centrifugation, the cell pellet was resuspended in washing medium. Recovery rates of nucleated cells (NC) and colony-forming cells (CFC) after the washing procedure were 97.0±9.7% and 103.8±20.1%, respectively.
Potassium and free hemoglobin were removed at 90.3% and 81.3%. Residual numbers of NC and CFC after the suspensions at room temperature for 2hr remained at 90.6±12.5% and 90.6±12.3%, respectively. These results indicate that the removal of cryoprotectant is effective in cord blood transplantation.抄録全体を表示PDF形式でダウンロード (725K) -
藤原 俊文, 二神 信夫, 砂川 浩, 讃井 将満, 大塚 祐史, 村山 隆紀, 谷口 良久, 瀬尾 憲正, 岩田 明子, 加藤 尚美, 飯 ...1998 年 44 巻 6 号 p. 693-696
発行日: 1998/12/01
公開日: 2010/03/12
ジャーナル フリーA new hepatitis-associated virus, hepatitis G virus (HGV), has been reported. Although its epidemiology, mode of transmission, and infectiousness are not yet well understood, it may be transmitted by transfusion. We investigated the transmission of HGV in 146 patients who underwent surgery, during which an average of 6.0±6.0 (range 1 to 35) units of blood was transfused. Transmission was determined through postoperative detection of HGV-RNA. Ten patients (6.8%) demonstrated HGV-RNA in the serum within 1 to 4 weeks after operation. Among these, HGV-RNA was detected in at least one of the blood units received by seven patients, all of whose units were available for testing. HGV-RNA was detected transiently for 2 to 3 weeks after surgery in two, and persisted after transfusion in the other eight. HGV viremia did not resolve in most of these patients and persisted longer than 16 months. None of them developed posttransfusion hepatitis. These results indicate that HGV can be transmitted by transfusion and that infection is permanent, but does not induce posttransfusion hepatitis.抄録全体を表示PDF形式でダウンロード (1498K) -
1998 年 44 巻 6 号 p. 735-736
発行日: 1998/12/01
公開日: 2010/03/12
ジャーナル フリーPDF形式でダウンロード (176K)
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