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Volume 53 (1986)
- Issue 6 Pages 543-
- Issue 5 Pages 433-
- Issue 4 Pages 321-
- Issue 3 Pages 225-
- Issue 2 Pages 125-
- Issue 1 Pages 3-
Volume 53, Issue 2
Displaying 1-14 of 14 articles from this issue
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1986 Volume 53 Issue 2 Pages 125-128
Published: April 15, 1986
Released on J-STAGE: July 10, 2009
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Masayasu Minami, Yoko Hirata1986 Volume 53 Issue 2 Pages 129-136
Published: April 15, 1986
Released on J-STAGE: July 10, 2009
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Makoto Yamashita1986 Volume 53 Issue 2 Pages 137-142_2
Published: April 15, 1986
Released on J-STAGE: December 04, 2009
JOURNAL FREE ACCESSMonoclonal antibody-coated cells form rosettes with ox erythrocytes coated with anti-mouse Ig. This rosetting technique for the enumeration of T lymphocyte subsets and the study of both phenotypic and morphological characteristics of leukemia and lymphoma cells was investigated.
With this method, the mean percentages of αLeu4 (pan T lymphocyte), αLeu2a (suppressor/cytotoxic T lymphocyte) and αLeu3a (helper/inducer T lymphocyte) positive cells of peripheral blood E+cells in healthy adults were 80.0±7.0%, 30.5±7.5% and 53.0±10.0%, respectively. The ratio of helper/suppressor T lymphocyte was 1.2-2.2. These values were similar to the published values determined by immunofluorescence assay and complement-mediated cytotoxicity assay.
In the malignant cell surface marker analysis, adult T-cell leukemia showed the phenotype of helper/inducer T lymphocytes (αLeu4+, 2a-, 3a+); T-cell lymphoma, that of common thymocyte (αLeu4+, 2a+, 3a+); acute lymphocytic leukemia, that of common ALL (αcALL+, HLA-DR+, αLeu4-, 2a-, 3a-, S-Ig-). In the case of AMMoL, the percentage of MY 4 positive cells was found to be 56%, that of MY 7, 72% and that of MY 9, 77%. In addition, the simultaneous analysis of cytological features and monoclonal antibody-defined surface markers of rosette-forming cells was achieved by preparing smears with cytochemical staining.
This method is technically simple, commonly available and as sensitive as other techniques. Therefore, we consider it to be of value in routine practice and well-suited for use in the characterization of malignant cells.View full abstractDownload PDF (2290K) -
Atsuo Narita1986 Volume 53 Issue 2 Pages 143-151
Published: April 15, 1986
Released on J-STAGE: December 04, 2009
JOURNAL FREE ACCESSThe effect of TRH on gastric antral motor activity was examined in human subjects. Antral motor activity was assessed by balloon-kymography and electromyography (EMG). A balloon and an electrode were introduced into the gastric antrum through 2 channel fiberscope. When TRH was administered iv bolus at a dose of 250 or 500μg, gastric peristalsis was completely inhibited for 5-10min. Time of occurrence of rhythmic contraction during recovery was not modulated by previous TRH injection. When TRH was infused iv at a rate of 4μg/min, gastric peristalsis was inhibited completely for as long as the subjects were receiving TRH. Reinfusion of TRH after a short period of cessation similarly inhibited gastric peristalsis. The inhibitory effect of TRH on gastric motor activity was also confirmed by endoscopy and polysogram of the stomach.
These results demonstrate that TRH administered iv exerts a potent inhibitory effect on gastric motor activity in human subjects and that the phenomenon of desentization and/or tachylaxis were not observed. Thus, TRH can be considered a useful adjunct to endoscopic and roentgenographic examinations of the stomach.View full abstractDownload PDF (12132K) -
Ryozo Kimura1986 Volume 53 Issue 2 Pages 152-158_7
Published: April 15, 1986
Released on J-STAGE: December 04, 2009
JOURNAL FREE ACCESSThe main purpose of this study was to investigate the chronological ultrastructural changes in the pancreas after experimental thermal injury.
Adult Wistar rats were used. The severe thermal burns of 3rd degree, about 30% of body surface, was installed on the back of the rats. After installationn of the thermal burns, the rats were sacrificed periodically at 5 minutes, 1 hour, 3 hours, 12 hours, 1, 2, 3, 7, 14 and 40 days. Each experimental group consisted of three rats and a total of thirty rats, was used.
In the acinar cells, degeneration of the ergastoplasm, swelling of the mitochondria, aggregate of the zymogen granules and appearance of the autophagic vesicle being digested by lysosomal enzyme were observed. The above-mentioned changes became obvious at 3 to 12 hours and then after they gradually disappeared. Concerning about the n-cells of Langerhans islets, vesiculation of granules, degeneration of the mitochondria and ergastoplasm were prominent at 12 hours after installation of burn. On the other hand, the a-cells were not involved.
Morphological evidences of circulatory insufficiency such as intracapillary stasis with interstitial edema due to burn shock both in acinar cell region and Langerhans islets were also obtained.View full abstractDownload PDF (17700K) -
Ryoichi Sato1986 Volume 53 Issue 2 Pages 159-173
Published: April 15, 1986
Released on J-STAGE: July 10, 2009
JOURNAL FREE ACCESSThe effects of intracellular acidification on the electrical activity of single ventricular cell were examined by internal dialysis technique through a patch clamp pipette.
Mild intracellular acidification (pHpip6.0)evoked a time-independent outward current and stronger acidification(pHpip5.0) caused reduction of all current systems of the membrane including the evoked outward current.The pHpip6.0-induced current was specific tointracellular acidification and was separated into twocomponents by thedifference of the effects of Cd2+ and Ba2+.Both components of the current could be diminished with the replacement of intracellular K+toCs+ and may be also carried byK+. A block of current systems by mild intracellular acidification (pHpip6.0) was confirmed in the case of the inward-rectifier K+ channel using a single channel recording in the inside-out patch.The ineffectiveness of low pHi(pHpip6.0)in the whole cell experiment could be explained by the existence of Na-H exchange system at the cell membrane, since even pHpip6.8could suppress the membrane current under Na-free Tyrodesolution.View full abstractDownload PDF (902K) -
Tetsuta Ihara1986 Volume 53 Issue 2 Pages 174-181
Published: April 15, 1986
Released on J-STAGE: July 10, 2009
JOURNAL FREE ACCESSCorticotropin-releasing factor (CRF)-like immunoreactivity was measured by radioimmunoassay in human organs and a small cell carcinoma of the lung associated with typical ectopic adrenooorticotropic hormone (ACTH) syndrome. It was found to be distributed in the stomach, pancreas, adrenal gland and a tumor producing ACTH ectopically in a concentration less than one twentieth of that of the hypothalamus. CRF-like immunoreactivity was not detected in the lung and thyroid tissue under the conditions tested. A dilution curve of CRF-like immunoreactivity in tissue extracts paralleled that of synthetic human CRF. When tissue extract was subjected to gel chromatography on a Sephadex G-50 column, a major immunoreactive CRF emmerged in elution volumes consistent with synthetic human CRF.
To examine whether CRF plays a role in the control of gut function possibly through modulation of other gut peptides, synthetic ovine CRF (100 μg iv bolus, followed by 200 μg infusion over 100 min) was administered to six normal subjects following ingestion of a 450 kcal meal. There was no change in plasma insulin, glucagon, gastrin, glucose and triglycerides compared to the control infusion.
These results suggest that a material (s) closely related to CRF is present in the stomach, pancreas, adrenal gland and a tumor producing ACTH ectopically. However, the role of CRF in these tissues remains to be studied.View full abstractDownload PDF (443K) -
Sumio Shin1986 Volume 53 Issue 2 Pages 182-190
Published: April 15, 1986
Released on J-STAGE: December 04, 2009
JOURNAL FREE ACCESSMeasurement of total blood volume (TBV) and red cell volume (RCV) is indispensable to understand the physiological background of hemodynamic change in newborn infants. A technique for measuring the blood volume of newborn infants was established by using non-radioactive 50Cr. Red blood cells (RBC) labeled with enriched stable isotope 50Cr were injected into the newborn infants. Blood samples were subsequently taken every few days. After the RBC portion was separated and dried, the dilution rate of the RBC was quantitated by neutron activation analysis.
The purpose of this investigation was to observe the change of TBV and RCV in full term neonates delivered in a sitting position. The effect of early and delayed cord clamping of the neonates upon TBV was also studied within the first 120 hours after birth. Studies were performed on 23 neonates. 10 were delivered in a sitting position, 7 in a supine position and 6 by cesarean section.
Serial measurements of TBV (RCV) in the neonates delivered in a sitting position whose cords were clamped early have shown an average volume of 63.4±4.8 (35.5±4.9)ml/kg at 1.5 hour, 94.4±11.8 (42.2±2.3)ml/kg at 24 hours and 86.0±10.0 (41.5±5.8)ml/kg at 72 hours after birth. The neonates whose cords were clamped late had about 20-30% higher TBV and RCV than the early-clamped neonates within 120 hours after birth. Under early cord clamping conditions, there was no significant difference in TBV and RCV just after birth between neonates delivered in a sitting position and those in a supine position.View full abstractDownload PDF (598K) -
Hisaaki Hatano1986 Volume 53 Issue 2 Pages 191-197
Published: April 15, 1986
Released on J-STAGE: December 04, 2009
JOURNAL FREE ACCESSPlasma GH response to human GH-releasing factor (GRF) was examined in adult female rats that had been treated with monosodium glutamate (MSG ; 4 mg/g BW, sc) for 5 days during the neonatal period. Rats given MSG had reduced nasal-anal length and revealed obesity compared to the control rats. Five months after MSG treatment, when the animals were killed by decapitation, it was found that their immunoreactive GRF content in the pituitary stalk-median eminence tissue was less than 20% of that of the control group and their anterior pituitary weight had decreased. However, the concentration of GH had not altered.
Under pentobarbital anesthesia, basal plasma GH levels were significantly reduced in MSG-treated rats as compared to the control rats. A significant dose-dependent plasma GH response was observed after the administration of two doses of human GRF (0.25 and 1 μg/kg BW, iv) in the control group and MSG-treated rats. The responses among MSG-treated rats did not differ from those in the control rats.
These results show that the pituitary's responsiveness to exogenous GRF is well preserved in MSG-treated rats despite prolonged and severe depletion of endogenous GRF.View full abstractDownload PDF (440K) -
Saburo Kameyama1986 Volume 53 Issue 2 Pages 198-205
Published: April 15, 1986
Released on J-STAGE: July 10, 2009
JOURNAL FREE ACCESSThe distribution of mechanical strength in the medial tibial condyle of frozen rheumatoid arthritis (RA) knees was investigated. Specimens were taken from 17 medial tibial condyle sites and the compressive strength was measured. The compressive strength distribution for the normal human knee was investigated using the same method. The results for the normal and RA knees were compared.
The compressive strength of the RA knee varied with the medial tibial condyle site. The greatest compressive strength occurred slightly anterior to the center. The compressive strength distribution of the RA knee was very similar to that of the normal knee, but the maximum value was only about 73% of the normal knee's. Involvement of RA activity, reduced activity in daily living (ADL) and especially disuse atrophy were suspected. Correlations of the patients' ages, RA duration, ADL, steroid use and load bearing with the compressive strength were further studied.View full abstractDownload PDF (401K) -
Kazuo Hirano, Kaisuke Ishihara, Saburo Kikuchi1986 Volume 53 Issue 2 Pages 206-208_1
Published: April 15, 1986
Released on J-STAGE: July 10, 2009
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Yoshiya Shinagawa, Miyako Kikuchi1986 Volume 53 Issue 2 Pages 209-211
Published: April 15, 1986
Released on J-STAGE: July 10, 2009
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Yasunori Hiraoka, Tsawtung Lin, Narumi Tsuboi, Yoshizo Nakagami1986 Volume 53 Issue 2 Pages 212-215
Published: April 15, 1986
Released on J-STAGE: July 10, 2009
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1986 Volume 53 Issue 2 Pages 216-219
Published: April 15, 1986
Released on J-STAGE: July 10, 2009
JOURNAL FREE ACCESSDownload PDF (281K)
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