Two dimensional echocardiography (2-DE) and ²⁰¹TI single photon emission computed tomography (SPECT) were performed in 51 patients with acute myocardial infarction (AMI) and the results were compared with those obtained by left ventriculography(LVG). After Loh et al., the image of the left ventricular (LV) wall was divided into 9 segments. LV wall motion and Ti up take in each segment were evaluated according to the irseverity and assigneds coresfrom 1 to 5. The degree of global abnormality in each patient as determined by each method was obtained by the sum of the scores assigned to the 9 segments, and expressed as ΣLVG, Σ2-DE or ΣSPECT, Σ′LVG, Σ′2-DE and Σ'SPECT were also measured for 5 LV segments which weree quivalent to the right anterior oblique view in LVG. Ejection fraction (EF) was measured by LVG using Kennedy's method.
The results may be summarized as follows:
1) The meanv alueo f Σ2-DE (Σ2-DE) w as almostt he samea s that of ΣLVG (ΣLVG), but that of ΣSPECT (ΣSPECT) was significantlyh igher than ΣLVG (p<0.05).
2) The correlation coefficient of ILVG with 12-DE was 0.85 (p<0.001) and with ISPECT, 0.72 (p<0.001).
3) ZING, 1'2-DE and Z′SPECT correlated significantly with EF (LVGr: = 0.82; 2 -DE: r =0.74; S PECT: r=0.63).
4) Using multiple regression analysis, ΣLVG and EF were estimated from the sum of the 2-DE and SPECT scores in each segment and expressed as ΣLVG and EF.
Inconclusion, LV wall motion and ejection fraction can be estimated precisely by noninvasive diagnostic methods such as 2-DE and SPECT.

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The effects of common bile duct ligation on hepatocytes in rats were investigated by light and electron microscopicatle chniques. C ytoskeletael lementss uch as actin and cytokeratinw ere immunohistochemically increasedi n liverc ellsa fter biled uct ligation. E lectronm icroscopicaolb servationr evealedt hat bile canaliculi were rich in microvilliin the controll iver, whilei n the experimentalr ats, the bile canaliculiw ere dilated. A decreased number of microvilli and bleb formations were also noted. In the cytoplasm, pericanalicular microfilamentsa nd intermediatef ilamentsw ere abundant. These findings suggest that increased biliary pressure may affect not only the hepatocellularp lasma membranec onstituents, b ut also the filamentous structure in the hepatocyteF. urthermoret he congestedb ile acida ffectm ayb e worsea fter a commonb ile duct obstruction.

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The VPA total level (T value), the free level (F value), and the free fraction (FF) were measured in 74 epileptic children 'under valproic acid (VPA) monotherapy. The relationship between the T vlue, blood collection time, therapy duration, serum free fatty acid, clinical features, F value and FF were then studied. Blood was taken either before the morning (pre-breakfast) administration of medicine (Cmin.), 2-3hours after the post-breakfast administration (Cmax.), or both. The subjects were divided into one Cmin. and one Cmax. group. The results of serum VPA measurement revealed hat T values in the Cmin. group ranged from 23.0 μg/ml to 113.0 μg/ml (average: 50.0±16.2 μg/ml), F values from 2.0 μg/ml to 16.0 μg/m_l (6.0±2.7 μg/ml) and FF from 5.9% to 21.7% (11.8±3.8%). In the Cmax. group, T values ranged from 41.0 μg/ml to 163.0 μg/ml (80.5±24.3 μg/ml), F values from 3.7 μg/ml to 22.8 μg/ml (10.0±4.2 μg/ml) and FF from 7.1% to 22.2% (12.2±3.3%). There was no difference in FF between the two groups. In both groups, T and F values significantly and positively correlated and FF was not affected by age or VPA therapy duration. However, FF varied in the early stage of medication. In individual cases with no seizures and receiving constant doses, the longer the period of medication, the greater the decrease in FF. Although free fatty acid was concurrently measured in some cases, it did not correlate with the F value or the FF. In 45 cases, changes in the FF were followed in both groups on the same day, but no general tendencies were noted. Diurnal fluctuation was studied in 4 cases. Significance of the clinical features was evaluated. The subjects were then classified by seizure type for group comparison and no differences in the FF among the different types were observed. During the follow-up period, 5 cases had seizures, but when their serum levels were compared with those of members of both groups, the T values did not differ. The F values and the FF in the 5 cases were below the mean values of the two groups. These findings suggest that when factors affecting VPA protein binding are expected to be present or when seizures cannot be controlled despite a sufficient T value in the blood, the F value measurement is of particular importance.

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A cell line (NMSG10) was established form malignant fibrous histiocytoma in human tibial bone. The cells revealed polymorphism at the primary culture stage, but they gradually became monotonous fibroblastic cells during transfer. On light microscopic examination, the features of these transformed cells were shown to be positive for alcian blue, oil red 0 and acid phosphatase stain.
In an immunohistochemical test, the cells were shown to be positive for antiproteoglycans, S-100, α₁- antitrypsin, vimentin and actin. An electron microscopic examination, revealed multiple irregular long microvilli extending from the cell surface. Also noted were microvesicles and lipid vacuoles in the cytoplasm. There was also a collagenous microfilament in the extracellular matrix. Furthermore, we found a lot of mucoidal substance which was able to be stained with alcian blue and to be digested by hyaluronidase in the cultured medium. This mucoidal substance was identifiedthough two dimensional electrophoresis as being mainly hyaluronic acid with a macromolecular weight, a small amount of heparan sulfate and chondroitin sulfate. Additionally, chromosomal analysis was performed at the 7th passage, revealing aneuploidy witha modal number of 46. The primary cells and NMSG10 cells were not transferable to nude mice.

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To establish the combination of chemotherapy with adoptive immunotherapy (AIT), using lymphokine activated killer cells (LAICa) nd/or interleukin-2 (IL-2) a, u thor examined the following:
1) Pretreatment with anticancer agents for peripheral blood mononuclear cells (PBMC) and its effect on LAIC cell cytotoxicity and cell yield.
2) The addition of anticancer agents in the induction phase to LAIC cell and its effect on LAIC cell cytotoxicity and cell yield.
3) Pretreatment with anticancer agents to induced LAICc ell and its effect on LAK cell cytotoxicity and cell yield.
4) The cytotoxicity of LAIC cell against tumor cells treated with anticancer agents.
Our experiment has shown that when we take peripheral blood mononuclear cells (PBMC) to induce LAK cells, there is no influence on its LAICc ell yield or its cytotoxicity after being harvested as LAICc ells, even if there is a maximum concentration of anticancer agents, but in the case of the LAICc ell induction phase VDS, CDDP, ADM and MMC have a significant effect on LAIC cell yield and on cytotoxicity of LAK cell after being arvested as LAIC cells.
And it has also been shown that, if there is a maximum concentration of anticancer agents, it has an effect on the induced LAK cell cytotoxicity and on the LAICc ell yield after being recultured with IL-2.
On the other hand, LAIC cell cytotoxicity makes no difference to tumor cells whether they are treated or not with anticancer agents.
These results suggest that we can take peripheral blood mononuclear cells to induce LAIC cells unrelated to the administration of anticancer agents, and that if we use a combination of chemotherapy with adoptive immunotherapy (IL-2 administration and/or LAK cell adoptation), we should start with the administration of anticancer agents and then administer IL-2 and/or transfer LAK cells after the concentration of anticancer agents decreased under 1/10 of maximum concentration in the blood level of our conventionally clinical use.

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The immunohistochemicloacl alizatioonf Na⁺, K⁺-ATPase and calmodulin was investigated in rat heart muscle using antiserum against the catalytic subunit of the human kidney Na⁺, K⁺-ATPase and bovine testis calmodulinat the electronm icroscopilce ve. l Immunostaininfgo r both Na⁺, K⁺-ATPase and calmodulin was noted on the plasmam embranea nd plasmalemmal vesicleos f the vasculare ndotheliuman dc ardiacm usclecellsC. almoduliwna sa lsod etectedin t he cytoplasmT. hesei mmunohistochemicloacl alizationws ereevaluatedb y immunobloatn alyse. s Such localizatioonf Na⁺, K⁺-ATPase and calmodulinsu ggeststh at they mayp laya n important rolei n the regulation of Na⁺-Ca⁺⁺ exchange across the cell membrane.

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Clinical significance of endoscopic examination in acute corrosive gastritis were evaluated. Serial endoscopic observations were made in men and rabbits with acute corrosive gastritis. The endoscopic findings were matched against the histological features. The most characteristic endoscopic finding was a brown coloration of the mucosa. Histologically, this corresponded to gangrenous mucosa which was accompaniedby necrosis of the gastric wall throughout its entire thickness. When the brown coloration of the mucosa became extensive, chemical peritonitis was induced by the leakage of caustic solution through the wall of the stomach. The recognition of this brown coloration of the mucosa was often hampered by blood coagula adhering to the mucosa or brown coloration of the mucosa caused by hematin formation. The latter disappeared 3-6 days after the ingestion of corrosives.
It was concluded that endoscopic examination in patients with acute corrosive gastritis should be performed to permit accurate recognition of brown coloration of the mucosa 4-7 days after the ingestion of corrosives.

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