The authors reported in the previous paper the confirmation of existence of 7 ferulates..... 1. campesteryl-, 2. stigmasteryl-, 3. β-sitosteryl-, 4. cycloartanyl-, 5, cycloartenyl-, 6. 24-methylenecycloartanyl- and 7. an unknown triterpenyl-ferulates..., ..in rice-bran oil by means of TLC and GLC.
The present report relates to the further confirmation of these ferulates (exclusive of the 2nd, the 4th and the 7th.) and their unsaponifiable matters by means of NMR and Mass Spectrography. Comparing to data of the chemical shifts of C-18 and C-19 protons in androstanes listed in the report of Bhacca et al., with those of sterines and triterpenes obtained by us, the conformations of the free triterpenes and the triterpenyl parts in their ferulates are not analogous, though the free sterins and the steryl parts in their ferulates are coincident.

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In the quantitative internal standardization method of fatty acids by gas liquid chromatography, calibration coefficients of response of each fatty acid (FA) must be obtained in preliminary. The authors found that this coefficient 'k' has some relative relation to carbon number and to relative retention time (RRT), when FID and TCD are used as detector, and DEGS as packing liquid. Namely, the straight lines were obserbed on the relation of k vs carbon number for capric to linoleic acid, while a curved lines were obtained on the relation of k vs RRT. Therefore, as the practical method, when k of some standard fatty acids were previously obtained from each calibration curve with the relation indicated in equation (1), followed by drawing of the standard curve of k vs carbon number or RRT, k of the other unknown fatty acids may be possible to obtained from these relations.
S/S₀=k · a/a₀ ……
S₀ : peak area of internal standard material.
S : peak area of each fatty acid.
a₀ : weight of internal standard material.
a : weight of each fatty acid.

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Fats from ruminants contain unsaturated trans-fatty acids but not for those of nonruminants. This characteristic feature was utilized for judgment of the purity of lard. A total of 20 samples including 11 kinds of lard, 5 of beef tallow, 3 of horse fat and 1 of mutton tallow were submitted to the test, and a comparative examination was made on the relationship between the amount of traps-acid and Bömer number with the mixture of lard and beef tallow.
1) Beef tallow contained 5.15.7 (x5.43%) and mutton tallow contained 10.8% of elaidic acid, and also an acid assumed to be vaccenic acid was found at an average of 1.02% in beef tallow and 1.4% in mutton tallow. This acid was not detected in ; lard or horse fat.
2) Beef tallow and mutton tallow exhibited numerous unidentified peaks in the pattern of the Apiezon L capillary column, and these peaks can be utilized for their detection, together with traps-acids.
3) In the mixture of lard and beef tallow, traps-acids can be detected as much as 5% of beef tallow is mixed, and when beef tallow is included in amount of 520%, peaks of unidentified components appear. These are more precise factors than the Bömer number. However, if horse fat is mixed in this case, it cannot be detected by this method, as well as with the Bömer number.

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The saturated, monoenic and hydroxy C₁₈-phosphono fatty acid esters in which a phosphono group was directly connected to the center of their long alkyl chains were synthesized by the radical addition reaction of diethyl phosphite with methyl oleate, linoleate and acetylated ricinoleate respectively. Then, all ester linkages of the phosphono fatty acid esters were hydrolyzed with hydrochloric acid. Properties and utilization of these phosphono fatty acids and their derivatives were discussed.
Trisodium salts of these phosphono fatty acids were more surface active than trisodium salt of 2-phosphonostearic acid, because the hydrophilic group was present at the center of hydrophobic alkyl chain.
Among these phosphono fatty acids, 9 (10) -phosphostearic acid from methyl oleate had good thermal stability and showed only a little change on thermogravimetric and a differential thermal analyses up to 250°C.
These phosphono fatty acidesters obtained were effective additives for improvement of cleanness of polyvinyl chloride sheet containing metal soap stabilizer. Moreover, it was found that methyl 9 (10) -diethylphosphonostearate was an excellent flame resisting additives for polyvinyl chloride.
The phosphono fatty acid esters thus produced were also effective as extreme-pressure additives of lubricating oil, and methyl diethylphosphono acetoxystearate from acetylated methyl ricinoleate was superior most, among these products.

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The addition reaction of propylene oxide to higher alcohol was investigated. Polyoxyethylene derivatives of polyoxypropylated lauryl ethers were prepared and the change in their surface activities, such as surface tension, foaming property, wetting power, dispersing power and emulsifying power, by the difference in the number of moles of propylene oxide, was discussed.
1) KOH and NaOH are excellent catalysts, more than 3 wt% of which must be added to alcohol, and the recomended reaction temperature is above 150 °C.
2) As compared with polyoxyethylene lauryl ethers, the surface tension and the cmc value of lauryl alcohol·propylene oxide·ethylene oxide adducts are lower and the dispersing power is superior, but the foaming property, the wetting power and the emulsifying power are inferior.

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Amounts of two kinds of nitrogen containing soils removed from naturally soiled cloth were estimated from peak height in elution curve of gel filtration using Sephadex G-100. The relation between amount of the removed soil and detergency efficiency evaluated by reflectance measurement was discussed. It was found that the amount of nitrogen containing soil can be equivocally related to the detergency efficiency independent of the kinds of surfactant and builder aqueous solutions used as washing solution. In conclusion it seems unnecessary to asume the specific interaction between proteins and anionic surfactants such as sodium alkyl benzene sulf onate with respect to the mechanism of removal of nitrogen containing soils.

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